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Evaluation of Legionella V-TesT for the detection of Legionella pneumophila antigen in urine samples

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Abstract

We evaluated a new immunochromatographic assay (Legionella V-TesT, Coris BioConcept, Gembloux, Belgium) for its ability to detect Legionella pneumophila serogroup 1 antigen in urine. Test devices were read at various time points to determine the optimum incubation time regarding performance. The results were compared with those obtained with the BinaxNOW urinary antigen test. The sensitivity and specificity were 82.2% and 98.6%, respectively, for the Legionella V-TesT and 83.9% and 100%, respectively, for the BinaxNOW urinary antigen test after 15 min of incubation. When tests were examined after 60 min, the sensitivity for both tests increased to 91.5%.

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Acknowledgements

We thank Coris BioConcept (Gembloux, Belgium) for supplying the assays evaluated in this study.

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Authors and Affiliations

  1. Regional Laboratory of Public Health, Haarlem, The Netherlands

    J. P. Bruin, E. P. F. IJzerman & B. M. W. Diederen

  2. Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands

    M. F. Peeters

Authors
  1. J. P. Bruin
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  2. M. F. Peeters
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  3. E. P. F. IJzerman
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  4. B. M. W. Diederen
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Corresponding author

Correspondence to B. M. W. Diederen.

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Bruin, J.P., Peeters, M.F., IJzerman, E.P.F. et al. Evaluation of Legionella V-TesT for the detection of Legionella pneumophila antigen in urine samples. Eur J Clin Microbiol Infect Dis 29, 899–900 (2010). https://doi.org/10.1007/s10096-010-0932-0

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  • DOI: https://doi.org/10.1007/s10096-010-0932-0

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