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Clinical relevance of cagPAI intactness in Helicobacter pylori isolates from Vietnam

European Journal of Clinical Microbiology & Infectious Diseases volume 29, pages 651–660 (2010)Cite this article

Abstract

The purpose of this paper is to investigate the relationship between clinical outcome and the intactness of cagPAI in Helicobacter pylori strains from Vietnam. The presence or absence of 30 cagPAI genes was investigated by polymerase chain reaction (PCR) and dot-blotting. H. pylori-induced interleukin-8 secretion and hummingbird phenotype, and H. pylori adhesion to gastric epithelial cells were examined. The serum concentration of pepsinogen 1, pepsinogen 2, and gastrin was also measured in all patients. cagPAI was present in all 103 Vietnamese H. pylori isolates, of which 91 had intact cagPAI and 12 contained only a part of cagPAI. Infection with the partial cagPAI strains was less likely to be associated with peptic ulcer and chronic gastric mucosal inflammation than infection with strains possessing intact cagPAI. The partial cagPAI strains lacked almost all ability to induce interleukin-8 secretion and the hummingbird phenotype in gastric cells. Their adhesion to epithelial cells was significantly decreased in comparison with intact cagPAI strains. Moreover, for the first time, we found an association between cagPAI status and the serum concentration of pepsinogens 1 and 2 in infected patients. H. pylori strains with internal deletion within cagPAI are less virulent and, thus, less likely to be associated with severe clinical outcomes.

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Acknowledgment

We thank Mrs. Yoko Kudou for her technical assistance.

Author information

Affiliations

  1. Department of Molecular Pathology, Faculty of Medicine, Oita University, Yufu City, Oita, 879-5593, Japan

    L. T. Nguyen, T. Uchida, Y. Tsukamoto & M. Moriyama

  2. Department of Gastroenterology, Faculty of Medicine, Oita University, Oita, Japan

    L. T. Nguyen, T. Okimoto, M. Kodama, K. Murakami & T. Fujioka

  3. Department of Human Environmental and Social Medicine, Faculty of Medicine, Oita University, Oita, Japan

    T. Uchida

  4. 108 Hospital, Hanoi, Vietnam

    T. D. Trinh, L. Ta, H. B. Mai & H. S. Le

  5. Cho Ray Hospital, Ho Chi Minh, Vietnam

    D. Q. D. Ho & H. H. Hoang

  6. Department of Gastrointestinal Endoscopy, Tama-Nagayama Hospital, Nippon Medical School, Tokyo, Japan

    T. Matsuhisa

  7. Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Oita, Japan

    Y. Yamaoka

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  1. L. T. Nguyen
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  2. T. Uchida
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Correspondence to M. Moriyama.

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Nguyen, L.T., Uchida, T., Tsukamoto, Y. et al. Clinical relevance of cagPAI intactness in Helicobacter pylori isolates from Vietnam. Eur J Clin Microbiol Infect Dis 29, 651–660 (2010). https://doi.org/10.1007/s10096-010-0909-z

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  • DOI: https://doi.org/10.1007/s10096-010-0909-z

Keywords

  • cagA
  • Gastric Epithelial Cell
  • Pylorus Strain
  • Pylorus Isolate
  • Gastric Mucosal Atrophy