Abstract
The aims of this study were to compare the clinical features of patients with extensively drug-resistant tuberculosis (XDRTB) and multidrug-resistant tuberculosis (MDRTB) and the genotypic characteristics of these Mycobacterium tuberculosis isolates. A total of 90 non-HIV-infected patients having MDRTB (n = 80, not including XDRTB, 88.9%) and XDRTB (n = 10, 11.1%) were identified from 2000 to 2007. Genotypes of the 39 available isolates were evaluated by spoligotyping and the 24-locus mycobacterial interspersed repetitive units–variable number of tandem repeats (MIRU-VNTR) scheme. Patients with XDRTB were more likely to have previous history of TB and cavitary lung lesions than patients with MDRTB (P < 0.05). Among the 39 isolates for spoligotyping analysis, the Beijing genotype was the most common (n = 21, 53.8%). Four (44.4%) isolates of XDRTB and 17 (56.7%) isolates of MDRTB belonged to Beijing family genotypes. There was no significant difference in the anti-tuberculosis drug resistance rates between Beijing and non-Beijing genotype isolates or in the clinical features of infected patients. In conclusion, significant differences in clinical manifestations existed among patients with XDRTB and MDRTB. The clinical features of patients infected with the Beijing genotype and the drug resistance profile of the Beijing genotype isolates were similar to those for the non-Beijing family genotype.
This is a preview of subscription content, log in via an institution to check access.
References
Lai CC, Tan CK, Huang YT et al (2008) Extensively-drug resistant Mycobacterium tuberculosis during a trend of decreasing drug resistance from 2000 through 2006 at a medical center in Taiwan. Clin Infect Dis 47:e57–e63
Jou R, Chiang CY, Huang WL (2005) Distribution of the Beijing family genotypes of Mycobacterium tuberculosis in Taiwan. J Clin Microbiol 43:95–100
Kamerbeek J, Schouls L, Kolk A et al (1997) Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol 35:907–914
Supply P (2005) Multilocus Variable Number Tandem Repeat Genotyping of Mycobacterium tuberculosis. Technical Guide. Institut de Biologie/Institut Pasteur de Lille, May 2005. Available online at: http://www.miru-vntrplus.org/MIRU/files/MIRU-VNTRtypingmanualv6.pdf
Kim HR, Hwang SS, Kim HJ et al (2007) Impact of extensive drug resistance on treatment outcomes in non-HIV-infected patients with multidrug-resistant tuberculosis. Clin Infect Dis 45:1290–1295
Jeon CY, Hwang SH, Min JH et al (2008) Extensively drug-resistant tuberculosis in South Korea: risk factors and treatment outcomes among patients at a tertiary referral hospital. Clin Infect Dis 46:42–49
Mitnick CD, Shin SS, Seung KJ et al (2008) Comprehensive treatment of extensively drug-resistant tuberculosis. N Engl J Med 359:563–574
Masjedi MR, Farnia P, Sorooch S et al (2006) Extensively drug-resistant tuberculosis: 2 years of surveillance in Iran. Clin Infect Dis 43:841–847
Mlambo CK, Warren RM, Poswa X et al (2008) Genotypic diversity of extensively drug-resistant tuberculosis (XDR-TB) in South Africa. Int J Tuberc Lung Dis 12:99–104
van Soolingen D, Qian L, de Haas PE et al (1995) Predominance of a single genotype of Mycobacterium tuberculosis in countries of East Asia. J Clin Microbiol 33:3234–3238
Acknowledgements
This study was partly supported by the Institute for Biotechnology and Medicine Industry, Taiwan (DOH97-DC-1501).
Transparency declarations
None to declare.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lai, C.C., Tan, C.K., Lin, S.H. et al. Clinical and genotypic characteristics of extensively drug-resistant and multidrug-resistant tuberculosis. Eur J Clin Microbiol Infect Dis 29, 597–600 (2010). https://doi.org/10.1007/s10096-010-0874-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10096-010-0874-6