Abstract
The Phenotype MicroArray™ (PM) technology was used to study the metabolic characteristics of 29 Salmonella strains belonging to seven serotypes of S. enterica spp. enterica. Strains of serotypes Typhimurium (six strains among definite phage types DTs 1, 40 and 104) and Agona (two strains) were tested for 949 substrates, Enteritidis (six strains of phage type PT1), Give, Hvittingfoss, Infantis and Newport strains (two of each) were tested for 190 substrates and seven other Agona strains for 95 substrates. The strains represented 18 genotypes in pulsed-field gel electrophoresis (PFGE). Among 949 substrates, 18 were identified that could be used to differentiate between the strains of those seven serotypes or within a single serotype. Unique metabolic differences between the Finnish endemic Typhimurium DT1 and Agona strains were detected, for example, in the metabolism of d-tagatose, d-galactonic acid γ-lactone and l-proline as a carbon source. Thus, the PM technique is a useful tool for identifying potential differential markers on a metabolic basis that could be used for epidemiological surveillance.
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Acknowledgements
Funding for this research was provided by The Academy of Finland, ELVIRA project (project number 117897).
The staff of the Department for Food and Environmental Safety at the Veterinary Laboratories Agency (VLA), Weybridge, Surrey, UK, is gratefully acknowledged for their teaching and giving of valuable information on the Phenotype MicroArray™ technology and Gareth Jones for his help in the statistical methods. The staff at the Bacteriology Unit, National Institute for Health and Welfare (THL), Helsinki, Finland, is gratefully acknowledged for their skilful technical assistance in conventional phenotyping and genotyping isolates of human origin. Susanna Lukinmaa is acknowledged for her help in finding the international names for the PFGE profiles.
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Kauko, T., Haukka, K., AbuOun, M. et al. Phenotype MicroArray™ in the metabolic characterisation of Salmonella serotypes Agona, Enteritidis, Give, Hvittingfoss, Infantis, Newport and Typhimurium. Eur J Clin Microbiol Infect Dis 29, 311–317 (2010). https://doi.org/10.1007/s10096-009-0859-5
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DOI: https://doi.org/10.1007/s10096-009-0859-5