Abstract
Rapid and reliable diagnostic methods are needed to control methicillin-resistant Staphylococcus aureus (MRSA) transmission. We studied the BD GeneOhm™ MRSA Assay which is based on one specific amplification product at the junction of the right extremity sequence of the staphylococcal cassette chromosome mec (SCCmec) and the chromosomal sequence of orfX of S. aureus. The test was applied on 95 clinical isolates in Finland: 83% were positive. The isolates giving negative results represented several pulsed-field gel electrophoresis (PFGE) types and harboured SCCmec types IV, V, VI or were new types with different combinations of ccr genes.
References
Cooper BS, Stone SP, Kibbler CC et al (2004) Isolation measures in the hospital management of methicillin resistant Staphylococcus aureus (MRSA): systematic review of the literature. BMJ 329:533–540. doi:10.1136/bmj.329.7465.533
Jonas D, Speck M, Daschner FD et al (2002) Rapid PCR-based identification of methicillin-resistant Staphylococcus aureus from screening swabs. J Clin Microbiol 40:1821–1823. doi:10.1128/JCM.40.5.1821-1823.2002
Fang H, Hedin G (2006) Use of cefoxitin-based selective broth for improved detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 44:592–594. doi:10.1128/JCM.44.2.592-594.2006
Huletsky A, Giroux R, Rossbach V et al (2004) New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J Clin Microbiol 42:1875–1884. doi:10.1128/JCM.42.5.1875-1884.2004
Drews SJ, Willey BM, Kreiswirth N et al (2006) Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core clinical laboratory setting. J Clin Microbiol 44:3794–3796. doi:10.1128/JCM.01509-06
Kerttula AM, Lyytikäinen O, Kardén-Lilja M et al (2007) Nationwide trends in molecular epidemiology of methicillin-resistant Staphylococcus aureus, Finland, 1997–2004. BMC Infect Dis 7:94–101. doi:10.1186/1471-2334-7-94
Murchan S, Kaufmann ME, Deplano A et al (2003) Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 41:1574–1585. doi:10.1128/JCM.41.4.1574-1585.2003
Chongtrakool P, Ito T, Ma XX et al (2006) Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements. Antimicrob Agents Chemother 50:1001–1012. doi:10.1128/AAC.50.3.1001-1012.2006
Thomas L, van Hal S, O’Sullivan M et al (2008) Failure of the BD GeneOhm StaphS/R Assay for identification of Australian methicillin-resistant Staphylococcus aureus strains: duplex assays as the ”gold standard” in settings of unknown SCCmec epidemiology. J Clin Microbiol 46:4116–4117. doi:10.1128/JCM.01146-08
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Sissonen, S., Pasanen, T., Salmenlinna, S. et al. Evaluation of a commercial MRSA assay when multiple MRSA strains are causing epidemics. Eur J Clin Microbiol Infect Dis 28, 1271–1273 (2009). https://doi.org/10.1007/s10096-009-0771-z
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DOI: https://doi.org/10.1007/s10096-009-0771-z