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Host species-specific translocation of Escherichia coli

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Abstract

The purpose of this paper is to investigate the rate of translocation of Escherichia coli strains in different experimental/animal models. Four proficient translocating E. coli strains isolated from mesenteric lymph nodes (MLNs) and/or the blood of rats (strains KIC-1 and KIC-2), from a fatal case of pancreatitis (HMLN-1) and from pigs (PC-1 isolated in this study) were tested for their ability to translocate across two host species and the Caco-2 cell line as a model of the human gut epithelium. HMLN-1 was found in the MLNs of all 15 pigs tested. This strain, however, did not translocate in any rats and only colonised the caecum of four rats in small numbers. HMLN-1 and PC-1 were the dominant translocating strains in Caco-2 cells compared to KIC-1 and KIC-2, which were found to translocate at a lower rate in pigs and in Caco-2 cells. The rate of translocation of PC-1 in rats was also very low compared to KIC-1 and KIC-2. We suggest that, in studies aiming to investigate the mechanism of translocation of E. coli strains isolated from humans, rats may not be an appropriate animal model and that the Caco-2 cells or pigs are more suitable in vitro and in vivo models, respectively.

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Authors and Affiliations

  1. Faculty of Science, Health and Education, University of the Sunshine Coast, Maroochydore DC, Queensland, 4556, Australia

    M. Katouli, N. L. Ramos, W. Robinson & H. M. Ison

  2. Department of Surgery, Karolinska Hospital, Stockholm, Sweden

    C. G. Nettelbladt

  3. Department of Surgery, University Hospital, Uppsala, Sweden

    M. Ljungdahl

  4. Microbiology and Tumorbiology Centre, Karolinska Institute, Stockholm, Sweden

    A. Brauner & R. Möllby

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  1. M. Katouli
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  2. N. L. Ramos
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  3. C. G. Nettelbladt
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  4. M. Ljungdahl
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  6. H. M. Ison
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  8. R. Möllby
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Correspondence to M. Katouli.

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Katouli, M., Ramos, N.L., Nettelbladt, C.G. et al. Host species-specific translocation of Escherichia coli . Eur J Clin Microbiol Infect Dis 28, 1095–1103 (2009). https://doi.org/10.1007/s10096-009-0754-0

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  • DOI: https://doi.org/10.1007/s10096-009-0754-0

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