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Epidemiological typing of clinical isolates of Achromobacter xylosoxidans: comparison of phenotypic and genotypic methods

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Abstract

The purpose of this paper is to evaluate the utility of different typing methods for Achromobacter xylosoxidans clinical isolates. Ninety-two blood culture isolates of A. xylosoxidans subsp. xylosoxidans were collected over a 25-month period. The typeability, discriminatory power and reproducibility of commonly used phenotypic and genotypic methods, such as resistotyping, plasmid profiling, whole-cell protein fingerprinting, random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE), were compared. All 92 isolates were typeable by all of the methods used, with comparable reproducibility. PFGE showed the highest discriminatory power (98.9%), but whole-cell protein profiling showed better correlation with epidemiological data without significant loss in discriminatory power (94%). Whole-cell protein profiling is a reliable epidemiological tool for the analysis of A. xylosoxidans; PFGE is the most discriminatory.

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  1. Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India

    M. Kaur, P. Ray, M. Bhatty & M. Sharma

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  1. M. Kaur
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  2. P. Ray
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  3. M. Bhatty
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Correspondence to P. Ray.

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Kaur, M., Ray, P., Bhatty, M. et al. Epidemiological typing of clinical isolates of Achromobacter xylosoxidans: comparison of phenotypic and genotypic methods. Eur J Clin Microbiol Infect Dis 28, 1023–1032 (2009). https://doi.org/10.1007/s10096-009-0740-6

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  • DOI: https://doi.org/10.1007/s10096-009-0740-6

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