Abstract
Bone allografts retrieved from multi-organ donors can be decontaminated with minimally aggressive methods. Therefore, we evaluated the efficacy of antibiotics and antiseptics in the decontamination of bone fragments actively contaminated with coagulase-negative staphylococci. Gentamicin (512/1,024 μg/mL), rifampicin (400/1,000 μg/mL), chlorhexidine in alcohol and chlorhexidine soap were tested with different contact times and temperatures and a delay in starting decontamination. Gentamicin-susceptible strains dried on bone could be removed by gentamicin 512 μg/mL after 19 h of contact, while strains not dried on bone could be eliminated by soaking bone for 60 min in gentamicin 512 μg/mL. Rifampicin-susceptible strains could be eliminated by soaking bone for 60 min in rifampicin 1,000 μg/mL. In none of the experimental conditions could gentamicin/rifampicin-resistant staphylococci be eliminated. Antiseptics could not eliminate staphylococci from bone. Different antibiotics need different protocols in order to decontaminate bone allografts.
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This study was granted by the Research Foundation—Flanders.
There are no known conflicts of interest associated with this publication.
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V.S.M. Saegeman is an Aspirant Researcher of the Fund for Scientific Research—Flanders.
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Saegeman, V.S.M., Ectors, N.L., Lismont, D. et al. Effectiveness of antibiotics and antiseptics on coagulase-negative staphylococci for the decontamination of bone allografts. Eur J Clin Microbiol Infect Dis 28, 813–816 (2009). https://doi.org/10.1007/s10096-009-0715-7
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DOI: https://doi.org/10.1007/s10096-009-0715-7