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Evaluation of the capability of the VITEK 2 system to detect extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates, in particular with the coproduction of AmpC enzymes

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Abstract

A total of 317 Klebsiella pneumoniae and 291 Escherichia coli nonduplicate isolates were tested by the VITEK 2 system to evaluate its capability to detect extended-spectrum β-lactamases (ESBLs) among putative ESBL-producing isolates, in particular those with the coproduction of AmpC enzymes. β-lactamases produced by the test isolates had been characterised. The sensitivity and specificity for ESBLs were 98.9% and 98.5%, respectively. Ninety of the isolates were AmpC (CMY-2, CMY-8 or DHA-1) and ESBL (SHV and/or CTX-M) coproducers, and 74 isolates (82.2%) of them were flagged as ESBL producers. Our study indicates that the VITEK 2 system is an acceptable tool for ESBL detection among K. pneumoniae and E. coli isolates for laboratories where both imported AmpC and ESBLs are prevalent.

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Acknowledgement

This study was supported by an unrestricted grant from the National Cheng Kung University Hospital, Tainan, Taiwan.

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Authors and Affiliations

  1. Department of Pathology, National Cheng Kung University Medical College and Hospital, 138 Sheng-Li Road, Tainan, 70428, Taiwan

    H. M. Chen, P. F. Tsai, J. Y. Wann & J. J. Yan

  2. Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University Medical College, 138 Sheng-Li Road, Tainan, 70428, Taiwan

    J. J. Wu

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  1. H. M. Chen
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  2. J. J. Wu
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  3. P. F. Tsai
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  4. J. Y. Wann
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  5. J. J. Yan
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Correspondence to J. J. Yan.

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Chen, H.M., Wu, J.J., Tsai, P.F. et al. Evaluation of the capability of the VITEK 2 system to detect extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates, in particular with the coproduction of AmpC enzymes. Eur J Clin Microbiol Infect Dis 28, 871–874 (2009). https://doi.org/10.1007/s10096-009-0713-9

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  • DOI: https://doi.org/10.1007/s10096-009-0713-9

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