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Occurrence of group A rotavirus mixed P genotypes infections in children living in Goiânia-Goiás, Brazil

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Abstract

Group A rotaviruses (RVA) are the main causing agents of acute gastroenteritis worldwide, having a great impact on childhood mortality in developing countries. The objective of this study was to identify RVA-positive fecal samples with mixed P genotypes by hemi-nested reverse transcriptase-polymerase chain reaction (RT-PCR), followed by sequencing confirmation. Our results showed that, from the 81 RVA-positive samples, 25 were positive for more than one P genotype by hemi-nested RT-PCR. Of these 25 samples, 12 (48%) had their mixed P genotypes confirmed by sequencing and, from these, 10 were identified as P[6]P[8], one as P[4]P[6], and one as P[4]P[6]P[8]. Our results confirm the occurrence of RVA mixed infections among children in Brazil and reinforce the importance of the constant monitoring of RVA circulating strains for the efficacy of control/prevention against these agents.

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References

  1. Parashar UD, Hummelman EG, Bresee JS et al (2003) Global illness and deaths caused by rotavirus disease in children. Emerg Infect Dis 9:565–572

    PubMed  Google Scholar 

  2. Kapikian AZ, Hoshino Y, Chanock RM (2001) Rotaviruses. In: Knipe DM, Howley PM (eds) Fields virology, 4th edn. Lippincott Williams & Wilkins, Philadelphia

    Google Scholar 

  3. Rao CD, Gowda K, Yugandar Reddy BS (2000) Sequence analysis of VP4 and VP7 genes of nontypeable strains identifies a new pair of outer capsid proteins representing novel P and G genotypes in bovine rotaviruses. Virology 276:104–113

    Article  PubMed  CAS  Google Scholar 

  4. Estes MK (2001) Rotaviruses and their replication. In: Knipe DM, Howley PM (eds) Fields virology, 4th edn. Lippincott Williams & Wilkins, Philadelphia

    Google Scholar 

  5. Rahman M, Matthijnssens J, Nahar S et al (2005) Characterization of a novel P[25],G11 human group a rotavirus. J Clin Microbiol 43:3208–3212

    Article  PubMed  CAS  Google Scholar 

  6. Martella V, Ciarlet M, Bányai K et al (2006) Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. Virology 346:301–311

    Article  PubMed  CAS  Google Scholar 

  7. Steyer A, Poljsak-Prijatelj M, Barlic-Maganja D et al (2007) Molecular characterization of a new porcine rotavirus P genotype found in an asymptomatic pig in Slovenia. Virology 359:272–282

    Article  Google Scholar 

  8. Khamrin P, Maneekarn N, Peerakome S et al (2007) Novel porcine rotavirus of genotype P[27] shares new phylogenetic lineage with G2 porcine rotavirus strain. Virology 361:243–252

    Article  PubMed  CAS  Google Scholar 

  9. Ramig RF (1997) Genetics of the rotaviruses. Annu Rev Microbiol 51:225–255

    Article  PubMed  CAS  Google Scholar 

  10. Palombo EA (2002) Genetic analysis of Group A rotaviruses: evidence for interspecies transmission of rotavirus genes. Virus Genes 24:11–20

    Article  PubMed  CAS  Google Scholar 

  11. Gentsch JR, Woods PA, Ramachandran M et al (1996) Review of G and P typing results from a global collection of rotavirus strains: implications for vaccine development. J Infect Dis 174:S30–S36

    PubMed  Google Scholar 

  12. Santos N, Hoshino Y (2005) Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 15:29–56

    Article  PubMed  Google Scholar 

  13. Fischer TK, Page NA, Griffin DD et al (2003) Characterization of incompletely typed rotavirus strains from Guinea-Bissau: identification of G8 and G9 types and a high frequency of mixed infections. Virology 311:125–133

    Article  PubMed  CAS  Google Scholar 

  14. Rennels MB, Glass RI, Dennehy PH et al (1996) Safety and efficacy of high-dose rhesus human reassortant rotavirus vaccines—report of the National Multicenter Trial. United States Rotavirus Vaccine Efficacy Group. Pediatrics 97:7–13

    PubMed  CAS  Google Scholar 

  15. Pereira HG, Azeredo RS, Leite JPG et al (1985) A combined enzyme immunoassay for rotavirus and adenovirus (EIARA). J Virol Methods 10:21–28

    Article  PubMed  CAS  Google Scholar 

  16. Pereira HG, Leite JPG, Azeredo RS et al (1983) An atypical rotavirus detected in a child with gastroenteritis in Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz 78:245–250

    PubMed  CAS  Google Scholar 

  17. Boom R, Sol CJA, Salimans MMM et al (1990) Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28:495–503

    PubMed  CAS  Google Scholar 

  18. Cardoso DDP, Fiaccadori FS, Souza MBLD et al (2002) Detection and genotyping of astroviruses from children with acute gastroenteritis from Goiânia, Goiás, Brazil. Med Sci Monit 8:CR624–CR628

    Google Scholar 

  19. Gentsch JR, Glass RI, Woods P et al (1992) Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 30:1365–1373

    PubMed  CAS  Google Scholar 

  20. Higgins DG, Sharp PM (1998) Clustal: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244

    Article  Google Scholar 

  21. Santos N, Soares CC, Volotão EM et al (2003) Surveillance of rotavirus strains in Rio de Janeiro, Brazil, from 1997 to 1999. J Clin Microbiol 41:3399–3402

    Article  PubMed  Google Scholar 

  22. Nielsen NM, Eugen-Olsen J, Aaby P et al (2005) Characterisation of rotavirus strains among hospitalised and non-hospitalised children in Guinea-Bissau, 2002 a high frequency of mixed infections with serotype G8. J Clin Virol 34:13–21

    Article  PubMed  CAS  Google Scholar 

  23. Fischer TK, Eugen-Olsen J, Pedersen AG et al (2005) Characterization of rotavirus strains in a Danish population: high frequency of mixed infections and diversity within the VP4 gene of P[8] strains. J Clin Microbiol 43:1099–1104

    Article  PubMed  CAS  Google Scholar 

  24. Mascarenhas JDP, Paiva FL, Barardi CRM et al (1998) Rotavirus G and P types in children from Belém, northern Brazil, as determined by RT-PCR: occurrence of mixed P type infections. J Diarrhoeal Dis Res 16:8–14

    PubMed  CAS  Google Scholar 

  25. Bányai K, Gentsch JR, Glass RI et al (2004) Eight-year survey of human rotavirus strains demonstrates circulation of unusual G and P types in Hungary. J Clin Microbiol 42:393–397

    Article  PubMed  Google Scholar 

  26. Souza MBLD, Rácz ML, Leite JPG et al (2003) Molecular and serological characterization of group A rotavirus isolates obtained from hospitalized children in Goiânia, Brazil, 1998–2000. Eur J Clin Microbiol Infect Dis 22:441–443

    Article  PubMed  CAS  Google Scholar 

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Acknowledgment

The authors thank the National Counsel of Technological and Scientific Development (CNPq) of Brazil for providing financial support.

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Authors and Affiliations

  1. Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Av Delenda Rezende de Melo Esquina com 1º Avenida s/n, Setor Universitário, Goiânia, Goias, 74605050, Brazil

    E. R. L. Freitas, F. S. Fiaccadori, M. Souza & D. D. P. Cardoso

  2. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goias, Brazil

    C. M. A. Soares & J. A. Parente

  3. Departamento de Pediatria e Puericultura, Faculdade de Medicina, Universidade Federal de Goiás, Goiânia, Goias, Brazil

    P. S. S. Costa

Authors
  1. E. R. L. Freitas
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  2. C. M. A. Soares
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  3. F. S. Fiaccadori
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  4. M. Souza
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  5. J. A. Parente
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  6. P. S. S. Costa
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  7. D. D. P. Cardoso
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Correspondence to E. R. L. Freitas.

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Freitas, E.R.L., Soares, C.M.A., Fiaccadori, F.S. et al. Occurrence of group A rotavirus mixed P genotypes infections in children living in Goiânia-Goiás, Brazil. Eur J Clin Microbiol Infect Dis 27, 1065–1069 (2008). https://doi.org/10.1007/s10096-008-0542-2

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  • DOI: https://doi.org/10.1007/s10096-008-0542-2

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