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Evaluation of non-automated techniques for phenotypic detection of VanA-type Staphylococcus aureus

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Abstract

The study presented here was performed to evaluate the ability of various nonautomated in vitro susceptibility testing methods to detect glycopeptide resistance in the three presently identified strains of VanA-type methicillin-resistant Staphylococcus aureus. To this end, MICs were determined according to the guidelines of the National Committee for Clinical Laboratory Standards, the Comité de l’Antibiogramme de la Société Française de Microbiologie and the British Society for Antimicrobial Chemotherapy and by using the E-test, agar disc diffusion, agar screening plates, and ATB galleries. In each of these methods, vancomycin was more efficient than teicoplanin for detecting glycopeptide resistance.

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Acknowledgements

We thank B. Bozdogan for technical help and R. Leclercq for reading the manuscript. S. aureus strains were obtained through the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) program supported under NIAID/NIH contract no. N01-AI-95359

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  1. Centre National de Référence de la Résistance aux Antibiotiques and Unité des Agents Antibactériens, Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France

    C. Girard-Blanc & P. Courvalin

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  1. C. Girard-Blanc
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  2. P. Courvalin
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Correspondence to P. Courvalin.

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Girard-Blanc, C., Courvalin, P. Evaluation of non-automated techniques for phenotypic detection of VanA-type Staphylococcus aureus. Eur J Clin Microbiol Infect Dis 24, 562–565 (2005). https://doi.org/10.1007/s10096-005-1374-y

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  • DOI: https://doi.org/10.1007/s10096-005-1374-y

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