Skip to main content

Molecular detection of Bartonella quintana DNA in the dental pulp of a homeless patient

Abstract

Dental pulp has been proposed as a suitable tissue sample for the identification of pathogenic organisms. Using PCR with two specific gene targets, Bartonella quintana DNA was detected in the dental pulp extracted from the tooth of a homeless patient. The patient had been bacteremic 6 months previously but was not when the tooth was sampled.

This is a preview of subscription content, access via your institution.

Fig. 1

References

  1. Anderson BE, Neuman MA (1997) Bartonella spp. as emerging human pathogens. Clin Microbiol Rev 10:203–219

    CAS  PubMed  Google Scholar 

  2. Maurin M, Raoult D (1996) Bartonella (Rochalimaea) quintana infections. Clin Microbiol Rev 9:273–292

    CAS  PubMed  Google Scholar 

  3. Spach DH, Koehler JE (1998) Bartonella-associated infections. Emerg Infect Dis 12:137–155

    CAS  Google Scholar 

  4. Brouqui P, La Scola B, Roux V, Raoult D (1999) Chronic Bartonella quintana bacteremia in homeless patients. N Engl J Med 340:184–189

    Article  CAS  PubMed  Google Scholar 

  5. Spach DH, Kanter AS, Dougherty MJ, Larson AM, Coyle MB, Brenner DJ, Swaminathan B, Matar GM, Welch DF, Root RK (1995) Bartonella (Rochalimaea) quintana bacteremia in inner-city patients with chronic alcoholism. N Engl J Med 332:424–428

    Article  CAS  PubMed  Google Scholar 

  6. Drancourt M, Mainardi JL, Brouqui P, Vandenesch F, Carta A, Lehnert F, Etienne J, Goldstein F, Acar J, Raoult D (1995) Bartonella (Rochalimaea) quintana endocarditis in three homeless men. N Engl J Med 332:419–423

    Article  CAS  PubMed  Google Scholar 

  7. Rolain JM, Foucault C, Guieu R, La Scola B, Brouqui P, Raoult D (2002) Bartonella quintana in human erythrocytes. Lancet 360:226–228

    Article  PubMed  Google Scholar 

  8. Rolain JM, Foucault C, Brouqui P, Raoult D (2003) Erythroblast cells as a target for Bartonella quintana in homeless people. Ann N Y Acad Sci 990:485–487

    CAS  PubMed  Google Scholar 

  9. Aboudharam G, La Scola B, Raoult D, Drancourt M (2000) Detection of Coxiella burnetii DNA in dental pulp during experimental bacteremia. Microb Pathog 28:249–254

    Article  CAS  PubMed  Google Scholar 

  10. Glick M, Trope M, Pliskin ME (1989) Detection of HIV in the dental pulp of a patient with AIDS. J Am Dent Assoc 119:649–650

    CAS  PubMed  Google Scholar 

  11. Drancourt M, Aboudharam G, Signoli M, Dutour O, Raoult D (1998) Detection of 400-year-old Yersinia pestis DNA in human dental pulp: an approach to the diagnosis of ancient septicemia. Proc Natl Acad Sci USA 95:12637–12640

    Article  CAS  PubMed  Google Scholar 

  12. Maurin M, Rolain JM, Raoult D (2002) Comparison of in-house and commercial slides for detection by immunofluorescence of immunoglobulins G and M against Bartonella henselae and Bartonella quintana. Clin Diagn Lab Immunol 9:1004–1009

    Article  CAS  PubMed  Google Scholar 

  13. Foucault C, Raoult D, Brouqui P (2003) Randomized open trial of gentamicin and doxycycline for eradication of Bartonella quintana from blood in patients with chronic bacteremia. Antimicrob Agents Chemother 47:2204–2207

    Article  CAS  PubMed  Google Scholar 

  14. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  15. Zeaiter Z, Fournier PE, Ogata H, Raoult D (2002) Phylogenetic classification of Bartonella species by comparing groEL sequences. Int J Syst Evol Microbiol 52:165–171

    CAS  PubMed  Google Scholar 

  16. Minnick MF, Sappington KN, Smitherman LS, Andersson SG, Karlberg O, Carroll JA (2003) Five-member gene family of Bartonella quintana. Infect Immun 71:814–821

    Article  CAS  PubMed  Google Scholar 

  17. Aboudharam G, Drancourt M, Raoult D (2004) Culture of C. burnetii from the dental pulp of experimentally infected guinea pigs. Microb Pathog 36:349–350

    Article  PubMed  Google Scholar 

  18. Foucault C, Barrau K, Brouqui P, Raoult D (2002) Bartonella quintana bacteremia among homeless people. Clin Infect Dis 35:684–689

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to D. Raoult.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Aboudharam, G., Fournier, PE., Drancourt, M. et al. Molecular detection of Bartonella quintana DNA in the dental pulp of a homeless patient. Eur J Clin Microbiol Infect Dis 23, 920–922 (2004). https://doi.org/10.1007/s10096-004-1244-z

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10096-004-1244-z

Keywords

  • Blood Culture
  • Dental Pulp
  • Tooth Extraction
  • Homeless People
  • Coxiella Burnetii