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In vitro activity of amphotericin B, fluconazole and voriconazole against 162 Cryptococcus neoformans isolates from Africa and Cambodia

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Abstract

In order to determine the potential role that various antifungal agents might have in the management of cryptococcosis in tropical areas, the in vitro susceptibility of Cryptococcus neoformans isolates from Africa (n=52) and Cambodia (n=110) to three antifungal agents (amphotericin B, fluconazole and voriconazole) were compared using the E-test method. The results of this study (i) confirm the value of the E-test for testing the in vitro susceptibility of C. neoformans towards voriconazole; (ii) provide the first evidence demonstrating good activity of amphotericin B, fluconazole and voriconazole against Cambodian isolates; and (iii) show there are differences in susceptibility between African and Asian C. neoformans isolates, with Cambodian isolates appearing less susceptible to the agents tested but with amphotericin B maintaining good activity.

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Acknowledgements

We thank Pfizer for kindly providing the voriconazole E-test strips and for help with the translation of this article. The 19 strains from Rwanda used in this study are available at “BCCM/IHEM biomedical fungi and yeasts collection, Scientific Institute of Public Health, Wytsan Street 14, 1050 Brussels, Belgium”.

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Authors and Affiliations

  1. Service de Parasitologie-Mycologie-Médecine Tropicale, Centre Hospitalier et Universitaire, 37000, Tours, France

    J. Chandenier, K. D. Adou-Bryn, C. Douchet, M. Thérizol-Ferly & D. Richard-Lenoble

  2. Institut Pasteur du Cambodge, Phnom Penh, Cambodia

    B. Sar & Y. Buisson

  3. Département de Parasitologie-Mycologie-Médecine Tropicale, FMSS, BP 4009, Libreville, Gabon

    M. Kombila

  4. Institut Scientifique de Santé Publique-Mycologie, Wytsman Street, 141050, Brussels, Belgium

    D. Swinne

Authors
  1. J. Chandenier
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  2. K. D. Adou-Bryn
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  3. C. Douchet
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  4. B. Sar
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  5. M. Kombila
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  6. D. Swinne
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  7. M. Thérizol-Ferly
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  8. Y. Buisson
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  9. D. Richard-Lenoble
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Corresponding author

Correspondence to J. Chandenier.

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Chandenier, J., Adou-Bryn, K.D., Douchet, C. et al. In vitro activity of amphotericin B, fluconazole and voriconazole against 162 Cryptococcus neoformans isolates from Africa and Cambodia. Eur J Clin Microbiol Infect Dis 23, 506–508 (2004). https://doi.org/10.1007/s10096-004-1136-2

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  • DOI: https://doi.org/10.1007/s10096-004-1136-2

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