Abstract
The study reported here was conducted in order to explore the mechanism of action of granulocyte colony-stimulating factor (G-CSF) in the treatment of Burkholderia pseudomallei infections (otherwise known as melioidosis). Use of G-CSF as an adjunct to antibiotics has been associated with decreasing mortality among patients with melioidosis in the tropical region of the Northern Territory of Australia. However, using an in vitro whole blood assay, no significant difference was detected in the bactericidal activity of samples obtained from dialysis patients, patients with type 2 diabetes mellitus and healthy controls, and there was no improvement following coincubation with G-CSF.
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Acknowledgements
BJC conceived the study design and provided funding, PD developed the assay, AC and PD conducted the experiments and analyzed the data, AC drafted the manuscript. All authors revised and contributed to the manuscript. We acknowledge the assistance of Dr. P. Lawton with recruitment of dialysis patients. AC is supported by a National Health and Medical Research Council Training Scholarship. We declare that the experiments described in this study conform to the laws of the Commonwealth of Australia and to Australian National Health and Medical Research Council guidelines.
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Cheng, A.C., Dasari, P. & Currie, B.J. Granulocyte Colony-Stimulating Factor and an In Vitro Whole Blood Model of Melioidosis. Eur J Clin Microbiol Infect Dis 23, 205–207 (2004). https://doi.org/10.1007/s10096-003-1088-y
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DOI: https://doi.org/10.1007/s10096-003-1088-y