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Protocol for the Accelerated Detection of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Strains from Blood Cultures

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Abstract

The study presented here was performed to evaluate an accelerated protocol for the early detection of organisms producing extended-spectrum β-lactamase (ESBL). The procedure involved testing isolates directly from positive blood-culture bottles, and a total of 40 clinical isolates (10 ESBL-producing and 10 non-ESBL-producing isolates of both Escherichia coli and Klebsiella pneumoniae) were used. The isolates were inoculated into blood cultures bottles and, upon growth signal, fluid from the bottle was cultured directly onto plates with combination discs containing cefotaxime or ceftazidime with and without clavulanate. Results were compared with those of standard methods for the detection of ESBL. High concordance between the two methods was found, and the direct test showed high sensitivity (95%) and specificity (100%). Use of this accelerated protocol may speed detection of the ESBL phenotype and thereby facilitate the early administration of appropriate antimicrobial therapy.

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Achnowledgment

This work was supported by a research grant from the United States-Israel Binational Science foundation, Jerusalem, Israel.

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Authors and Affiliations

  1. Laboratory for Molecular Epidemiology and Antimicrobial Resistance, Tel-Aviv Sourasky Medical Center, 6 Weizmann Street, Tel Aviv 64239, Israel

    S. Navon-Venezia & A. Leavitt

  2. Department of Infectious Diseases, Tel-Aviv Sourasky Medical Center, 6 Weizmann Street, Tel Aviv 64239, Israel

    R. Ben-Ami

  3. Department of Epidemiology, Tel-Aviv Sourasky Medical Center, 6 Weizmann Street, Tel Aviv 64239, Israel

    M. J. Schwaber

  4. Laboratory of Microbiology, Tel-Aviv Sourasky Medical Center, 6 Weizmann Street, Tel Aviv 64239, Israel

    D. Schwartz

  5. Departments of Epidemiology and Infectious Diseases, Tel-Aviv Sourasky Medical Center, 6 Weizmann Street, Tel Aviv 64239, Israel

    Y. Carmeli

Authors
  1. S. Navon-Venezia
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  2. R. Ben-Ami
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  3. M. J. Schwaber
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  4. A. Leavitt
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  5. D. Schwartz
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  6. Y. Carmeli
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Corresponding author

Correspondence to S. Navon-Venezia.

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Navon-Venezia, S., Ben-Ami, R., Schwaber, M.J. et al. Protocol for the Accelerated Detection of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Strains from Blood Cultures. Eur J Clin Microbiol Infect Dis 23, 200–202 (2004). https://doi.org/10.1007/s10096-003-1086-0

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  • DOI: https://doi.org/10.1007/s10096-003-1086-0

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