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Molecular and Serological Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Goiânia, Brazil, 1998–2000

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Abstract

Fecal samples positive for rotavirus group A (n=120) were analyzed by enzyme immunoassay-monoclonal antibody (EIA-MAb) serotyping and/or reverse transcription/multiplex polymerase chain reaction (PCR) amplification to determine the prevalence of the [P] and G genotypes. The most prevalent G genotype/serotype detected was G1 (76.7%), followed by G2 (5.0%). Six samples were characterized as G9 by multiplex PCR, and one sample was characterized as G3 by EIA-MAb. The combinations of [P] and G genotypes found were P[8] and G1 (20.8%), P[6] and G1 (10.8%), P[6] and G9 (4.2%), P[8] and G2 (1.7%), and P[6] and G2 (0.8%). The diversity of rotavirus group A [P] and G genotypes/serotypes reinforces the need for continuous characterization of rotaviruses circulating in populations in Brazil.

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Authors and Affiliations

  1. Laboratório de Virologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua Delenda Rezende de Melo Esquina Com 1a Avenida s/n, Setor Universitário, 74605-050, Goiânia, Goiás, Brazil

    M. B. L. D. Souza, R. M. B. Martins & D. D. P. Cardoso

  2. Laboratório de Virologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Lineu Prestes, ICB2, São Paulo, Brazil

    M. L. Rácz & V. Munford

  3. Laboratório de Virologia Comparada, Departamento de Virologia, Fundação Oswaldo Cruz 4365, 21045-900, Rio de Janeiro, Brazil

    J. P. G. Leite

  4. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas I, UFG, Campus II, CEP 74001-970, Goiânia, Goiás, Brazil

    C. M. A. Soares

Authors
  1. M. B. L. D. Souza
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  2. M. L. Rácz
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  3. J. P. G. Leite
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  4. C. M. A. Soares
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  5. R. M. B. Martins
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  6. V. Munford
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  7. D. D. P. Cardoso
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Correspondence to M. B. L. D. Souza.

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Souza, M.B.L.D., Rácz, M.L., Leite, J.P.G. et al. Molecular and Serological Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Goiânia, Brazil, 1998–2000. Eur J Clin Microbiol Infect Dis 22, 441–443 (2003). https://doi.org/10.1007/s10096-003-0952-0

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  • DOI: https://doi.org/10.1007/s10096-003-0952-0

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