Abstract
The optimum conditions for β-thujaplicin production in a Cupressus lusitanica cell suspension culture were investigated. The conditions required for β-thujaplicin production were clearly different from the conditions for cell growth. The initial phosphate concentration and pH did not affect β-thujaplicin production. A total nitrogen source concentration higher than 3.2 mM suppressed production due to the presence of the ammonium ion. β-Thujaplicin production was observed at 95 mg/l without adding the ammonium ion to the medium. Strict control of major inorganic nutrients was not necessary to produce β-thujaplicin. This finding seems to be favorable for future automated production of β-thujaplicin in commercial cell culture plants.
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Received: October 3, 2001 / Accepted: February 20, 2002
Part of this report was presented at the 10th International Symposium on Wood and Pulp Chemistry, Yokohama, Japan, June 1999
Correspondence to:K. Fujita
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Yamada, J., Fujita, K. & Sakai, K. Effect of major inorganic nutrients on β-thujaplicin production in a suspension culture of Cupressus lusitanica cells. J Wood Sci 49, 172–175 (2003). https://doi.org/10.1007/s100860300027
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DOI: https://doi.org/10.1007/s100860300027