Skip to main content

Advertisement

SpringerLink
Log in

LncRNA SERPINB9P1 expression and polymorphisms are associated with ischemic stroke in a Chinese Han population

  • Original Article
  • Published:
Neurological Sciences Aims and scope Submit manuscript

Abstract

Long noncoding RNAs (lncRNAs) were reported to play important roles in the pathogenesis of ischemic stroke (IS). Our study aimed to investigate the role of lncRNA SERPINB9P1 expression in ischemic stroke and the association between SERPINB9P1 polymorphisms and IS risk, as well as examine the correlation of SERPINB9P1 expression and variants with clinical parameters of IS. The SERPINB9P1 levels in human participants and oxygen–glucose deprivation (OGD)–treated human A172 cells were measured by qRT-PCR. The SERPINB9P1 polymorphisms (rs375556 and rs318429) were genotyped by the MassARRAY platform. We found that the SERPINB9P1 expression was significantly downregulated in patients with IS compared with that in healthy controls. On the 14th day in the hospital, the SERPINB9P1 level in patients with moderate and severe stroke was significantly downregulated compared with the normal group. After stratification by gender, the rs375556 polymorphism was significantly associated with susceptibility to female IS in the recessive model, and the significant association remained after adjusting for age. After adjusting for gender and age, rs318429 was significantly associated with FPG and D-D levels, and rs375556 was significantly associated with INR and PTA levels in IS cases. Besides, the lncRNA SERPINB9P1 expressed downregulated in OGD/reoxygenation-treated human A172 cells. In conclusion, the lncRNA SERPINB9P1 may protect against cerebral ischemia–reperfusion injury and neurological impairment after IS. The SERPINB9P1 rs375556 polymorphism was associated with susceptibility to female IS, and SERPINB9P1 polymorphisms may influence the metabolism of blood glucose and regulation of coagulation function in patients with IS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Akhter MS, Biswas A, Abdullah SM, Behari M, Saxena R (2017) The role of PAI-1 4G/5G promoter polymorphism and its levels in the development of ischemic stroke in young Indian population. Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis 23:1071–1076

    Article  Google Scholar 

  2. Baek JY, Yeo HY, Chang HJ, Kim KH, Kim SY, Park JW, Park SC, Choi HS, Kim DY, Oh JH (2014) Serpin B5 is a CEA-interacting biomarker for colorectal cancer. Int J Cancer 134:1595–1604

    Article  PubMed  Google Scholar 

  3. Buchthal B, Weiss U, Bading H (2018) Post-injury nose-to-brain delivery of activin A and serpinB2 reduces brain damage in a mouse stroke model. Molecular therapy: the journal of the American Society of Gene Therapy 26:2357–2365

    Article  Google Scholar 

  4. Cabezas-Llobet N, Camprubi S, Garcia B, Alberch J, Xifro X (2018) Human alpha 1-antitrypsin protects neurons and glial cells against oxygen and glucose deprivation through inhibition of interleukins expression. Biochim Biophys Acta 1862:1852–1861

    Article  Google Scholar 

  5. Campos MA, Lascano J (2014) alpha1 Antitrypsin deficiency: current best practice in testing and augmentation therapy. Ther Adv Respir Dis 8:150–161

    Article  PubMed  Google Scholar 

  6. Cheng H, Sun M, Wang ZL, Wu Q, Yao J, Ren G, Sun XL (2020) LncRNA RMST-mediated miR-107 transcription promotes OGD-induced neuronal apoptosis via interacting with hnRNPK. Neurochemistry international 133:104644.

  7. Cheng X, Xu J, Gu M, Wang M, Sun B, Li Z, Ni G, Wang G, Weng Z, Shi Y, Zhang Z, Liu X (2018) Genetic variants in ALDH2 predict risk of ischemic stroke in a Chinese population. Gene 678:49–54

    Article  PubMed  Google Scholar 

  8. Choi SW, Kim BB, Choi DH, Park G, Shin BC, Song H, Kim D, Kim DM (2017) Stroke or left atrial thrombus prediction using antithrombin III and mean platelet volume in patients with nonvalvular atrial fibrillation. Clin Cardiol 40:1013–1019

    Article  PubMed  PubMed Central  Google Scholar 

  9. Cucchiara B, George DK, Kasner SE, Knutsson M, Denison H, Ladenvall P, Amarenco P, Johnston SC (2019) Disability after minor stroke and TIA: a secondary analysis of the SOCRATES trial. Neurology 93:e708–e716

    Article  PubMed  Google Scholar 

  10. Deming Y, Dumitrescu L, Barnes LL, Thambisetty M, Kunkle B, Gifford KA, Bush WS, Chibnik LB, Mukherjee S, De Jager PL, Kukull W, Huentelman M, Crane PK, Resnick SM, Keene CD, Montine TJ, Schellenberg GD, Haines JL, Zetterberg H, Blennow K, Larson EB, Johnson SC, Albert M, Moghekar A, Del Aguila JL, Fernandez MV, Budde J, Hassenstab J, Fagan AM, Riemenschneider M, Petersen RC, Minthon L, Chao MJ, Van Deerlin VM, Lee VM, Shaw LM, Trojanowski JQ, Peskind ER, Li G, Davis LK, Sealock JM, Cox NJ, Alzheimer’s Disease Neuroimaging I, Alzheimer Disease Genetics C, Goate AM, Bennett DA, Schneider JA, Jefferson AL, Cruchaga C, Hohman TJ, (2018) Sex-specific genetic predictors of Alzheimer’s disease biomarkers. Acta Neuropathol 136:857–872

    Article  PubMed  PubMed Central  Google Scholar 

  11. Ferreira LE, Secolin R, Lopes-Cendes I, Cabral NL, Franca PHC (2019) Association and interaction of genetic variants with occurrence of ischemic stroke among Brazilian patients. Gene 695:84–91

    Article  PubMed  Google Scholar 

  12. Flores AE, Pascotini ET, Kegler A, Gabbi P, Bochi GV, Barbisan F, Duarte T, Prado ALC, Duarte M, da Cruz IBM, Moresco RN, Santos ARS, Bresciani G, Royes LFF, Fighera MR (2017) ALA16VAL-MnSOD gene polymorphism and stroke: association with dyslipidemia and glucose levels. Gene 627:57–62

    Article  PubMed  Google Scholar 

  13. Flossmann E, Schulz UG, Rothwell PM (2004) Systematic review of methods and results of studies of the genetic epidemiology of ischemic stroke. Stroke 35:212–227

    Article  PubMed  Google Scholar 

  14. Gao N, Tang H, Gao L, Tu G, Luo H, Xia Y (2020) CYP3A4 and CYP11A1 variants are risk factors for ischemic stroke: a case control study. BMC Neurol 20:77

    Article  PubMed  PubMed Central  Google Scholar 

  15. Ge Y (2020) The clinical value of serum cystatin C and coagulation function test in patients with acute cerebral infarction. Electronic Journal of General Stomatology 7:174–175

    Google Scholar 

  16. Ghaedi H, Zare A, Omrani MD, Doustimotlagh AH, Meshkani R, Alipoor S, Alipoor B (2018) Genetic variants in long noncoding RNA H19 and MEG3 confer risk of type 2 diabetes in an Iranian population. Gene 675:265–271

    Article  PubMed  Google Scholar 

  17. Gu L, Huang J, Li J, Huang S, Li M, Gong L, Li T, Su L (2018) Association of CALM1 rs3179089 Polymorphism with ischemic stroke in Chinese Han population. NeuroMol Med 20:271–279

    Article  Google Scholar 

  18. Heit C, Jackson BC, McAndrews M, Wright MW, Thompson DC, Silverman GA, Nebert DW, Vasiliou V (2013) Update of the human and mouse SERPIN gene superfamily. Hum Genomics 7:22

    Article  PubMed  PubMed Central  Google Scholar 

  19. Hu X, Zan X, Xie Z, Li Y, Lin S, Li H, You C (2017) Association between plasminogen activator inhibitor-1 genetic polymorphisms and stroke susceptibility. Mol Neurobiol 54:328–341

    Article  PubMed  Google Scholar 

  20. Huang J, Yang J, Li J, Chen Z, Guo X, Huang S, Gu L, Su L (2019) Association of long noncoding RNA H19 polymorphisms with the susceptibility and clinical features of ischemic stroke in southern Chinese Han population. Metab Brain Dis 34:1011–1021

    Article  PubMed  Google Scholar 

  21. Hurley MJ, Durrenberger PF, Gentleman SM, Walls AF, Dexter DT (2015) Altered expression of brain proteinase-activated receptor-2, trypsin-2 and serpin proteinase inhibitors in Parkinson’s disease. Journal of molecular neuroscience: MN 57:48–62

    Article  PubMed  Google Scholar 

  22. Hurth H, Birkenhauer U, Steiner J, Schlak D, Hennersdorf F, Ebner FH (2020) Delayed cerebral ischemia in patients with aneurysmal subarachnoid hemorrhage - serum D-dimer and C-reactive protein as early markers. Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 29:104558.

  23. Ishii N, Ozaki K, Sato H, Mizuno H, Susumu S, Takahashi A, Miyamoto Y, Ikegawa S, Kamatani N, Hori M, Satoshi S, Nakamura Y, Tanaka T (2006) Identification of a novel non-coding RNA, MIAT, that confers risk of myocardial infarction. J Hum Genet 51:1087–1099

    Article  PubMed  Google Scholar 

  24. Kim JW, Lee SH, Ko HM, Kwon KJ, Cho KS, Choi CS, Park JH, Kim HY, Lee J, Han SH, Ignarro LJ, Cheong JH, Kim WK, Shin CY (2011) Biphasic regulation of tissue plasminogen activator activity in ischemic rat brain and in cultured neural cells: essential role of astrocyte-derived plasminogen activator inhibitor-1. Neurochem Int 58:423–433

    Article  PubMed  Google Scholar 

  25. Krishnamurthi RV, Ikeda T, Feigin VL (2020) Global, regional and country-specific burden of ischaemic stroke, intracerebral haemorrhage and subarachnoid haemorrhage: a systematic analysis of the global burden of disease study 2017. Neuroepidemiology 54:171–179

    Article  PubMed  Google Scholar 

  26. Liang Y, Zhang R, Zhang S, Ji G, Shi P, Yang T, Liu F, Feng J, Li C, Guo D, Chen M (2017) Association of ACYP2 and TSPYL6 genetic polymorphisms with risk of ischemic stroke in Han Chinese population. Mol Neurobiol 54:5988–5995

    Article  PubMed  Google Scholar 

  27. Liu C, Yang J, Zhang C, Liu M, Geng X, Ji X, Du H, Zhao H (2018) Analysis of long non-coding RNA expression profiles following focal cerebral ischemia in mice. Neurosci Lett 665:123–129

    Article  PubMed  Google Scholar 

  28. Lucas A, Yaron JR, Zhang L, Ambadapadi S (2018) Overview of Serpins and Their Roles in Biological Systems. Methods Mol Biol 1826:1–7

    Article  PubMed  Google Scholar 

  29. Malik R, Chauhan G, Traylor M, Sargurupremraj M, Okada Y, Mishra A, Rutten-Jacobs L, Giese AK, van der Laan SW, Gretarsdottir S, Anderson CD, Chong M, Adams HHH, Ago T, Almgren P, Amouyel P, Ay H, Bartz TM, Benavente OR, Bevan S, Boncoraglio GB, Brown RD, Jr., Butterworth AS, Carrera C, Carty CL, Chasman DI, Chen WM, Cole JW, Correa A, Cotlarciuc I, Cruchaga C, Danesh J, de Bakker PIW, DeStefano AL, den Hoed M, Duan Q, Engelter ST, Falcone GJ, Gottesman RF, Grewal RP, Gudnason V, Gustafsson S, Haessler J, Harris TB, Hassan A, Havulinna AS, Heckbert SR, Holliday EG, Howard G, Hsu FC, Hyacinth HI, Ikram MA, Ingelsson E, Irvin MR, Jian X, Jimenez-Conde J, Johnson JA, Jukema JW, Kanai M, Keene KL, Kissela BM, Kleindorfer DO, Kooperberg C, Kubo M, Lange LA, Langefeld CD, Langenberg C, Launer LJ, Lee JM, Lemmens R, Leys D, Lewis CM, Lin WY, Lindgren AG, Lorentzen E, Magnusson PK, Maguire J, Manichaikul A, McArdle PF, Meschia JF, Mitchell BD, Mosley TH, Nalls MA, Ninomiya T, O’Donnell MJ, Psaty BM, Pulit SL, Rannikmae K, Reiner AP, Rexrode KM, Rice K, Rich SS, Ridker PM, Rost NS, Rothwell PM, Rotter JI, Rundek T, Sacco RL, Sakaue S, Sale MM, Salomaa V, Sapkota BR, Schmidt R, Schmidt CO, Schminke U, Sharma P, Slowik A, Sudlow CLM, Tanislav C, Tatlisumak T, Taylor KD, Thijs VNS, Thorleifsson G, Thorsteinsdottir U, Tiedt S, Trompet S, Tzourio C, van Duijn CM, Walters M, Wareham NJ, Wassertheil-Smoller S, Wilson JG, Wiggins KL, Yang Q, Yusuf S, Consortium AF, Cohorts for H, Aging Research in Genomic Epidemiology C, International Genomics of Blood Pressure C, Consortium I, Starnet, Bis JC, Pastinen T, Ruusalepp A, Schadt EE, Koplev S, Bjorkegren JLM, Codoni V, Civelek M, Smith NL, Tregouet DA, Christophersen IE, Roselli C, Lubitz SA, Ellinor PT, Tai ES, Kooner JS, Kato N, He J, van der Harst P, Elliott P, Chambers JC, Takeuchi F, Johnson AD, BioBank Japan Cooperative Hospital G, Consortium C, Consortium E-C, Consortium EP-I, International Stroke Genetics C, Consortium M, Neurology Working Group of the CC, Network NSG, Study UKYLD, Consortium M, Sanghera DK, Melander O, Jern C, Strbian D, Fernandez-Cadenas I, Longstreth WT, Jr., Rolfs A, Hata J, Woo D, Rosand J, Pare G, Hopewell JC, Saleheen D, Stefansson K, Worrall BB, Kittner SJ, Seshadri S, Fornage M, Markus HS, Howson JMM, Kamatani Y, Debette S, Dichgans M (2018) Multiancestry genome-wide association study of 520,000 subjects identifies 32 loci associated with stroke and stroke subtypes. Nat Genet 50:524–537

    Article  PubMed  Google Scholar 

  30. Mangan MS, Melo-Silva CR, Luu J, Bird CH, Koskinen A, Rizzitelli A, Prakash M, Scarff KL, Mullbacher A, Regner M, Bird PI (2017) A pro-survival role for the intracellular granzyme B inhibitor Serpinb9 in natural killer cells during poxvirus infection. Immunol Cell Biol 95:884–894

    Article  PubMed  Google Scholar 

  31. Meadows KL (2018) Ischemic stroke and select adipose-derived and sex hormones: a review. Hormones 17:167–182

    Article  PubMed  Google Scholar 

  32. Mehta SL, Kim T, Vemuganti R (2015) Long noncoding RNA FosDT promotes ischemic brain injury by interacting with REST-associated chromatin-modifying proteins. The Journal of neuroscience: the official journal of the Society for Neuroscience 35:16443–16449

    Article  Google Scholar 

  33. Meschia JF, Nalls M, Matarin M, Brott TG, Brown RD, Jr., Hardy J, Kissela B, Rich SS, Singleton A, Hernandez D, Ferrucci L, Pearce K, Keller M, Worrall BB, Siblings With Ischemic Stroke Study I (2011) Siblings with ischemic stroke study: results of a genome-wide scan for stroke loci. Stroke 42:2726–2732

    Article  Google Scholar 

  34. Mukhopadhyay S, Johnson TA, Sarkar R, Antalis TM (2018) Serpins in venous thrombosis and venous thrombus resolution. Methods Mol Biol 1826:197–211

    Article  PubMed  PubMed Central  Google Scholar 

  35. Neurology Working Group of the Cohorts for H, Aging Research in Genomic Epidemiology Consortium tSGN, the International Stroke Genetics C (2016) Identification of additional risk loci for stroke and small vessel disease: a meta-analysis of genome-wide association studies. The Lancet Neurology 15:695–707

    Article  Google Scholar 

  36. Pan JX (2017) LncRNA H19 promotes atherosclerosis by regulating MAPK and NF-kB signaling pathway. Eur Rev Med Pharmacol Sci 21:322–328

    PubMed  Google Scholar 

  37. Petrone AB, O’Connell GC, Regier MD, Chantler PD, Simpkins JW, Barr TL (2016) The role of arginase 1 in post-stroke immunosuppression and ischemic stroke severity. Transl Stroke Res 7:103–110

    Article  PubMed  Google Scholar 

  38. Poorthuis MH, Algra AM, Algra A, Kappelle LJ, Klijn CJ (2017) Female- and male-specific risk factors for stroke: a systematic review and meta-analysis. JAMA Neurol 74:75–81

    Article  PubMed  Google Scholar 

  39. Praetner M, Zuchtriegel G, Holzer M, Uhl B, Schaubacher J, Mittmann L, Fabritius M, Furst R, Zahler S, Funken D, Lerchenberger M, Khandoga A, Kanse S, Lauber K, Krombach F, Reichel CA (2018) Plasminogen activator inhibitor-1 promotes neutrophil infiltration and tissue injury on ischemia-reperfusion. Arterioscler Thromb Vasc Biol 38:829–842

    Article  PubMed  Google Scholar 

  40. Reyahi A, Nik AM, Ghiami M, Gritli-Linde A, Ponten F, Johansson BR, Carlsson P (2015) Foxf2 is required for brain pericyte differentiation and development and maintenance of the blood-brain barrier. Dev Cell 34:19–32

    Article  PubMed  Google Scholar 

  41. Sanad EF, Hamdy NM, El-Etriby AK, Sebak SA, El-Mesallamy HO (2017) Peripheral leucocytes and tissue gene expression of granzyme B/perforin system and serpinB9: impact on inflammation and insulin resistance in coronary atherosclerosis. Diabetes Res Clin Pract 131:132–141

    Article  PubMed  Google Scholar 

  42. Tan RY, Markus HS (2015) Monogenic causes of stroke: now and the future. J Neurol 262:2601–2616

    Article  PubMed  Google Scholar 

  43. van der Burgh R, Meeldijk J, Jongeneel L, Frenkel J, Bovenschen N, van Gijn M, Boes M (2016) Reduced serpinB9-mediated caspase-1 inhibition can contribute to autoinflammatory disease. Oncotarget 7:19265–19271

    Article  PubMed  PubMed Central  Google Scholar 

  44. Wang J, Zhao H, Fan Z, Li G, Ma Q, Tao Z, Wang R, Feng J, Luo Y (2017) Long noncoding RNA H19 Promotes neuroinflammation in ischemic stroke by driving histone deacetylase 1-dependent M1 microglial polarization. Stroke 48:2211–2221

    Article  PubMed  Google Scholar 

  45. Wang L, Liu J, Yang Y, Peng B, Wang Y (2018) Report on stroke prevention and treatment in China 2017. Chinese Journal of Cerebrovascular Disease 15:611–617

    Google Scholar 

  46. Wang Y, Liu J, Wu H, Cai Y (2018) Combined biomarkers composed of environment and genetic factors in stroke. Biosci Trends 12:360–368

    Article  PubMed  Google Scholar 

  47. Wei YS, Xiang Y, Liao PH, Wang JL, Peng YF (2016) An rs4705342 T>C polymorphism in the promoter of miR-143/145 is associated with a decreased risk of ischemic stroke. Sci Rep 6:34620

    Article  PubMed  PubMed Central  Google Scholar 

  48. Williams FM, Carter AM, Hysi PG, Surdulescu G, Hodgkiss D, Soranzo N, Traylor M, Bevan S, Dichgans M, Rothwell PM, Sudlow C, Farrall M, Silander K, Kaunisto M, Wagner P, Saarela O, Kuulasmaa K, Virtamo J, Salomaa V, Amouyel P, Arveiler D, Ferrieres J, Wiklund PG, Ikram MA, Hofman A, Boncoraglio GB, Parati EA, Helgadottir A, Gretarsdottir S, Thorsteinsdottir U, Thorleifsson G, Stefansson K, Seshadri S, DeStefano A, Gschwendtner A, Psaty B, Longstreth W, Mitchell BD, Cheng YC, Clarke R, Ferrario M, Bis JC, Levi C, Attia J, Holliday EG, Scott RJ, Fornage M, Sharma P, Furie KL, Rosand J, Nalls M, Meschia J, Mosely TH, Evans A, Palotie A, Markus HS, Grant PJ, Spector TD, Euro CI, Wellcome Trust Case Control C, Monica Risk GA, Monograph, MetaStroke, International Stroke Genetics C (2013) Ischemic stroke is associated with the ABO locus: the EuroCLOT study. Ann Neurol 73:16–31

    Article  PubMed  Google Scholar 

  49. Wu N, Chen X, Cai T, Wu L, Xiang Y, Zhang M, Li Y, Song Z, Zhong L (2015) Association of inflammatory and hemostatic markers with stroke and thromboembolic events in atrial fibrillation: a systematic review and meta-analysis. Can J Cardiol 31:278–286

    Article  PubMed  Google Scholar 

  50. Wu W, Asakawa T, Yang Q, Zhao J, Lu L, Luo Y, Gong P, Han S, Li W, Namba H, Wang L (2017) Effects of neuroserpin on clinical outcomes and inflammatory markers in Chinese patients with acute ischemic stroke. Neurol Res 39:862–868

    Article  PubMed  Google Scholar 

  51. Wzorek J, Karpinski M, Wypasek E, Michalski M, Szczudlik A, Malinowski KP, Undas A (2018) Alpha-2-antiplasmin Arg407Lys polymorphism and cryptogenic ischemic cerebrovascular events: association with neurological deficit. Neurol Neurochir Pol 52:352–358

    Article  PubMed  Google Scholar 

  52. Xu X, Zhuang C, Chen L (2020) Exosomal long non-coding RNA expression from serum of patients with acute minor stroke. Neuropsychiatr Dis Treat 16:153–160

    Article  PubMed  PubMed Central  Google Scholar 

  53. Yang J, Gu L, Guo X, Huang J, Chen Z, Huang G, Kang Y, Zhang X, Long J, Su L (2018) LncRNA ANRIL expression and ANRIL gene polymorphisms contribute to the risk of ischemic stroke in the Chinese Han population. Cell Mol Neurobiol 38:1253–1269

    Article  PubMed  Google Scholar 

  54. Yi X, Lin J, Wang C, Zhou Q (2017) CYP genetic variants, CYP metabolite levels, and neurologic deterioration in acute ischemic stroke in Chinese population. Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 26:969–978

    Article  Google Scholar 

  55. Young JL, Sukhova GK, Foster D, Kisiel W, Libby P, Schonbeck U (2000) The serpin proteinase inhibitor 9 is an endogenous inhibitor of interleukin 1beta-converting enzyme (caspase-1) activity in human vascular smooth muscle cells. J Exp Med 191:1535–1544

    Article  PubMed  PubMed Central  Google Scholar 

  56. Zhang H, Yin Y, Zhang C, Zhang D (2019) Costs of hospitalization for stroke from two urban health insurance claims data in Guangzhou City, southern China. BMC Health Serv Res 19:671

    Article  PubMed  PubMed Central  Google Scholar 

  57. Zhang J, Yuan L, Zhang X, Hamblin MH, Zhu T, Meng F, Li Y, Chen YE, Yin KJ (2016) Altered long non-coding RNA transcriptomic profiles in brain microvascular endothelium after cerebral ischemia. Exp Neurol 277:162–170

    Article  PubMed  Google Scholar 

  58. Zhang Y, Cheng X, Liang H, Jin Z (2018) Long non-coding RNA HOTAIR and STAT3 synergistically regulate the cervical cancer cell migration and invasion. Chem Biol Interact 286:106–110

    Article  PubMed  Google Scholar 

  59. Zhang Y, Zhang Y (2020) lncRNA ZFAS1 improves neuronal injury and inhibits inflammation, oxidative stress, and apoptosis by sponging miR-582 and upregulating NOS3 expression in cerebral ischemia/reperfusion injury. Inflammation 43:1337–1350

    Article  PubMed  Google Scholar 

  60. Zheng Z, Liu S, Wang C, Han X (2018) A functional polymorphism rs145204276 in the promoter of long noncoding RNA GAS5 is associated with an increased risk of ischemic stroke. Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 27:3535–3541

    Article  Google Scholar 

  61. Zhou M, Wang H, Zeng X, Yin P, Zhu J, Chen W, Li X, Wang L, Wang L, Liu Y, Liu J, Zhang M, Qi J, Yu S, Afshin A, Gakidou E, Glenn S, Krish VS, Miller-Petrie MK, Mountjoy-Venning WC, Mullany EC, Redford SB, Liu H, Naghavi M, Hay SI, Wang L, Murray CJL, Liang X (2019) Mortality, morbidity, and risk factors in China and its provinces, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet 394:1145–1158

    Article  PubMed  PubMed Central  Google Scholar 

  62. Zhu M, Li N, Luo P, Jing W, Wen X, Liang C, Tu J (2018) Peripheral blood leukocyte expression of lncRNA MIAT and its diagnostic and prognostic value in ischemic stroke. Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 27:326–337

    Article  Google Scholar 

  63. Zhu R, Liu X, He Z (2018) Long non-coding RNA H19 and MALAT1 gene variants in patients with ischemic stroke in a northern Chinese Han population. Mol Brain 11:58

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The present study was funded by the National Natural Science Foundation of China (Nos. 81573756 and 81473670).

Author information

Author notes

  1. Jiao Huang, Lulu Zhu, and Xinyi Zhao contributed equally to this work and shared co-first authorship

Authors and Affiliations

  1. School of Public Health of Guangxi Medical University, 22 Shuangyong Road, Nanning, 530021, Guangxi, China

    Jiao Huang, Lulu Zhu, Xulong Wu, Jialei Yang, Bingyi Xu, Zhi Zhao & Li Su

  2. Department of Respiratory Disease Control, Institute of Infectious Disease Control and Prevention, Guizhou Provincial Center for Disease Control and Prevention, Guiyang, Guizhou Province, China

    Jiao Huang

  3. Guangxi University of Chinese Medicine, Nanning, Guangxi, China

    Xinyi Zhao

  4. Department of Internal Neurology, First Affiliated Hospital, Guangxi University of Chinese Medicine, 89-9 Dongge Road, Nanning, 530023, Guangxi, China

    Lian Gu

Authors
  1. Jiao Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lulu Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xinyi Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xulong Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jialei Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bingyi Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhi Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Lian Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Li Su
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Lian Gu or Li Su.

Ethics declarations

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the ethics committee of the Guangxi University of Chinese Medicine and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Huang, J., Zhu, L., Zhao, X. et al. LncRNA SERPINB9P1 expression and polymorphisms are associated with ischemic stroke in a Chinese Han population. Neurol Sci 43, 1143–1154 (2022). https://doi.org/10.1007/s10072-021-05418-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10072-021-05418-5

Keywords

Search

Navigation