Neurological Sciences

, Volume 39, Issue 4, pp 691–698 | Cite as

Association of MTHFR gene polymorphisms with migraine in North Indian population

  • Sukhvinder Kaur
  • Arif Ali
  • Anil Kumar Pandey
  • Balkirat Singh
Original Article


Polymorphisms in MTHFR gene are mostly associated with increased levels of homocysteine in the absence of dietary folate and are a risk factor for complex neurovascular diseases like migraine. The aim of present case-control study was to determine the association between MTHFR gene polymorphisms (C667T; rs 1801133, A1298C; rs 1801131) with migraine susceptibility. In total, 100 patients with migraine (23with MA and 77 with MO) and age-sex matched 100 healthy controls were included in this study from OPD of ESIC Medical College & Hospital, Faridabad. Genotyping was done by PCR-RFLP method. Genotypic and allelic frequencies were compared by SPSS 24 version. Genotypic results indicated a non-significant increase in frequencies of CT and TT in C667T SNP in migraine patients with control (52 and 10% vs. 42 and 7%: p > 0.05), but CC genotype in A1298C was found to be a risk factor in migraine patients than controls (30 vs. 17% respectively: p < 0.05). On comparing migraine subclasses, migraine with aura (MA) and without aura (MO) with control groups, the present study suggests that in MTHFR polymorphisms, the prevalence of 677CT genotype and T allele in C667T SNP influences susceptibility to MA (p < 0.05) but not to MO. Meanwhile, CC genotype in A1298C SNP could be a risk factor for migraine patients without aura (p < 0.05).


Migraine MTHFR gene rs 1801133 rs 1801131 Single nucleotide polymorphisms 



The authors sincerely thank Dr. Praveen Malik of ESIC Medical College & Hospital, Faridabad, India, for providing samples. Financial support from UGC, New Delhi, India, to Sukhvinder kaur as a UGC-PDF is also highly acknowledged.


This work was supported by PDF given to Sukhvinder Kaur by Union Grant Commission (UGC), New Delhi, India.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag Italia S.r.l., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiosciencesUGC-PDF, Gene Expression LabNew DelhiIndia
  2. 2.Department of BiosciencesUGC-BSR-FFNew DelhiIndia
  3. 3.Department of PhysiologyESIC Medical College & HospitalFaridabadIndia
  4. 4.NC Medical College & HospitalPanipatIndia

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