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Dynamic study of the hippocampal volume by structural MRI in a rat model of depression

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Abstract

The reduction of hippocampal volume remains controversial in depression because of the variability among individuals in clinical studies. Here, a reliable experimental rat model of depression, established by chronic unpredictable mild stress (CUMS), was used. Thirty rats were randomly divided into two groups (CUMS group and control group). Hippocampal volume was dynamically measured every 2 weeks in a 56-day chronic stress procedure using structural magnetic resonance imaging, and the correlation between the hippocampal volume and the learning and memory changes was investigated. Our results demonstrated that CUMS rats showed significantly smaller volumes of the bilateral hippocampus compared to that of the controls, changing dramatically with the development of CUMS procedure. The left hippocampal volume was reduced earlier and more markedly than the right one from the 2nd week to the 8th week of the CUMS procedure (on the 8th week: left: approximately 15.3 %; right: approximately 8.4 % reduction). Additionally, the hippocampal volume of CUMS rats was significantly negatively correlated with the learning and memory changes. Of note, it showed that the more obviously the hippocampal volume reduced, the more severely the learning and memory damaged. In conclusion, the hippocampal volume decreased gradually and dynamically and was correlated with the impairment of the learning and memory in depression.

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References

  1. Duman RS (2009) Neuronal damage and protection in the pathophysiology and treatment of psychiatric illness: stress and depression. Dialogues Clin Neurosci 11:239–255

    PubMed Central  PubMed  Google Scholar 

  2. Lee T, Jarome T, Li SJ et al (2009) Chronic stress selectively reduces hippocampal volume in rats: a longitudinal magnetic resonance imaging study. NeuroReport 20:1554–1558

    Article  PubMed Central  PubMed  Google Scholar 

  3. Eichenbaum H (2004) Hippocampus: cognitive processes and neural representations that underlie declarative memory. Neuron 44:109–120

    Article  CAS  PubMed  Google Scholar 

  4. Olton DS, Paras BC (2001) Spatial memory and hippocampal function. Neuropsychologia 17:669–682

    Article  Google Scholar 

  5. Koolschijn P, van Haren NE, Lensvelt Mulders GJ et al (2009) Brain volume abnormalities in major depressive disorder: a meta-analysis of magnetic resonance imaging studies. Hum Brain Mapp 30:3719–3735

    Article  PubMed  Google Scholar 

  6. Segev A, Rubin AS, Abush H et al (2014) Cannabinoid receptor activation prevents the effects of chronic mild stress on emotional learning and LTP in a rat model of depression. Neuropsychopharmacology 39:919–933

  7. Xing Y, He J, Hou J et al (2013) Gender differences in CMS and the effects of antidepressant venlafaxine in rats. Neurochem Int 63:570–575

    Article  CAS  PubMed  Google Scholar 

  8. Willner P (1997) Validity, reliability and utility of the chronic mild stress model of depression: a 10-year review and evaluation. Psychopharmacology 134:319–329

    Article  CAS  PubMed  Google Scholar 

  9. Willner P, Towell A, Sampson D et al (1987) Reduction of sucrose preference by chronic unpredictable mild stress, and its restoration by a tricyclic antidepressant. Psychopharmacology 93:358–364

    Article  CAS  PubMed  Google Scholar 

  10. Xi G, Hui J, Zhang Z et al (2011) Learning and memory alterations are associated with hippocampal N-acetylaspartate in a rat model of depression as measured by 1H-MRS. PLoS ONE 6:e28686

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Porsolt RD, Le PM, Jalfre M (1977) Depression: a new animal model sensitive to antidepressant treatments. Nature 266:730–732

    Article  CAS  PubMed  Google Scholar 

  12. Wolf OT, Dyakin V, Vadasz C et al (2002) Volumetric measurement of the hippocampus, the anterior cingulate cortex, and the retrosplenial granular cortex of the rat using structural MRI. Brain Res Protoc 10:41–46

    Article  CAS  Google Scholar 

  13. Posener JA, Wang L, Price JL et al (2003) High-dimensional mapping of the hippocampus in depression. Am J Psychiatr 160:83–89

    Article  PubMed  Google Scholar 

  14. Sheline YI, Wang PW, Gado MH et al (1996) Hippocampal atrophy in recurrent major depression. Proc Natl Acad Sci USA 93:3908–3913

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  15. Steffens DC, Byrum CE, McQuoid DR et al (2000) Hippocampal volume in geriatric depression. Biol Psychiatry 48:301–309

    Article  CAS  PubMed  Google Scholar 

  16. Bremner JD, Narayan M, Anderson ER et al (2000) Hippocampal volume reduction in major depression. Am J Psychiatr 157:115–118

    Article  CAS  PubMed  Google Scholar 

  17. Uno H, Tarara R, Else JG et al (1989) Hippocampal damage associated with prolonged and fatal stress in primates. J Neurosci 9:1705–1711

    CAS  PubMed  Google Scholar 

  18. Park J, Lee S, Oh C (2013) Treadmill exercise exerts ameliorating effect on isolation-induced depression via neuronal activation. J Exerc Rehabil 9:234–242

    Article  PubMed Central  PubMed  Google Scholar 

  19. Sapolsky RM (2000) Glucocorticoids and hippocampal atrophy in neuropsychiatric disorders. Arch Gen Psychiatry 57:925–935

    Article  CAS  PubMed  Google Scholar 

  20. Krištofiková Z, Kozmiková I, Hovorková P et al (2008) Lateralization of hippocampal nitric oxide mediator system in people with Alzheimer disease, multi-infarct dementia and schizophrenia. Neurochem Int 53:118–125

    Article  PubMed  Google Scholar 

  21. Pruessner JC, Li LM, Serles W et al (2000) Volumetry of hippocampus and amygdala with high-resolution MRI and three-dimensional analysis software: minimizing the discrepancies between laboratories. Cereb Cortex 10:433–442

    Article  CAS  PubMed  Google Scholar 

  22. Krištofiková Z, Št’Astný F, Bubeníková V et al (2004) Age-and sex-dependent laterality of rat hippocampal cholinergic system in relation to animal models of neurodevelopmental and neurodegenerative disorders. Neurochem Res 29:671–680

    Article  PubMed  Google Scholar 

  23. Samara A, Vougas K, Papadopoulou A et al (2011) Proteomics reveal rat hippocampal lateral asymmetry. Hippocampus 21:108–119

    Article  CAS  PubMed  Google Scholar 

  24. Spasojevic N, Jovanovic P, Dronjak S (2013) Molecular basis of chronic stress-induced hippocampal lateral asymmetry in rats and impact on learning and memory. Acta Physiol Hung 100:388–394

    Article  CAS  PubMed  Google Scholar 

  25. McEwen BS, Sapolsky RM (1995) Stress and cognitive function. Curr Opin Neurobiol 5:205–216

    Article  CAS  PubMed  Google Scholar 

  26. de Kloet ER, Joels M, Holsboer F (2005) Stress and the brain: from adaptation to disease. Nat Rev Neurosci 6:463–475

    Article  PubMed  Google Scholar 

  27. Sousa N, Lukoyanov NV, Madeira MD (2000) Reorganization of the morphology of hippocampal neurites and synapses after stress-induced damage correlates with behavioral improvement. Neuroscience 97:253–266

    Article  CAS  PubMed  Google Scholar 

  28. Song L, Che W, Min-Wei W et al (2006) Impairment of the spatial learning and memory induced by learned helplessness and chronic mild stress. Pharmacol Biochem Behav 83:186–193

    Article  CAS  PubMed  Google Scholar 

  29. Sun M, Alkon DL (2004) Induced depressive behavior impairs learning and memory in rats. Neuroscience 129:129–139

    Article  CAS  PubMed  Google Scholar 

  30. Jarrard LE (1993) On the role of the hippocampus in learning and memory in the rat. Behav Neural Biol 60:9–26

    Article  CAS  PubMed  Google Scholar 

  31. Squire LR (1992) Memory and the hippocampus: a synthesis from findings with rats, monkeys, and humans. Psychol Rev 99:195–231

    Article  CAS  PubMed  Google Scholar 

  32. Sousa N, Lukoyanov NV, Madeira MD et al (2000) Reorganization of the morphology of hippocampal neurites and synapses after stress-induced damage correlates with behavioral improvement. Neuroscience 97:253–266

    Article  CAS  PubMed  Google Scholar 

  33. Yin J, Turner GH, Lin H et al (2011) Deficits in spatial learning and memory is associated with hippocampal volume loss in aged apolipoprotein E4 mice. J Alzheimers Dis 27:89–98

    CAS  PubMed  Google Scholar 

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Acknowledgments

This study was supported by projects of Jiangsu Province Health Department (No. H200951); projects of Wuxi Municipal Technology Bureau (No. CSEW1N1111); projects of Wuxi Health Bureau (No. ML201320). We would like to thank Prof. Wang Jichen at Nanjing Medical University and Ma Tieliang M.D at Yixing People hospital for their valuable help.

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Authors and Affiliations

  1. Department of Radiology, The Affiliated Yixing Hospital of Jiangsu University, 75 Tongzhenguan Road, Wuxi, 214200, Jiangsu, China

    Yifeng Luo, Zhihong Cao & Liwei Wu

  2. Department of Medical Imaging, The Affiliated Hospital of Jiangsu University, Zhenjiang, China

    Dongqing Wang, Yuefeng Li, Weibin Sun & Yan Zhu

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  1. Yifeng Luo
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  2. Zhihong Cao
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  3. Dongqing Wang
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  4. Liwei Wu
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  5. Yuefeng Li
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  6. Weibin Sun
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  7. Yan Zhu
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Correspondence to Zhihong Cao.

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Luo, Y., Cao, Z., Wang, D. et al. Dynamic study of the hippocampal volume by structural MRI in a rat model of depression. Neurol Sci 35, 1777–1783 (2014). https://doi.org/10.1007/s10072-014-1837-y

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  • DOI: https://doi.org/10.1007/s10072-014-1837-y

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