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TRPC3 channel mediates excitation of striatal cholinergic interneurons

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Abstract

Striatal cholinergic interneurons exhibit tonic firing and more positive membrane potentials, however, the mechanism is unclear. In the present study, we found that intracellular perfusion of TRPC3 antibody induced outward current in striatal cholinergic interneurons identified by electrophysiological characteristics. The TRPC3 channel blocker flufenamic acid induced hyperpolarization, and reduced firing rate and outward current which was similar to the effect of TRPC3 channel antibody. Furthermore, by using single-cell RT-PCR we confirmed the co-existence of TRPC3 channel and D5 receptor mRNA in striatal cholinergic interneurons identified by electrophysiological characteristics and expression of choline acetyltransferase (Chat) mRNA. These results implied that the TRPC3 channel is involved in modulating the depolarization of cholinergic interneurons.

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Author notes

  1. Yu-Feng Xie

    Present address: Department of Pharmacology and Therapeutic, University of Manitoba, 753 McDermot Avenue, Winnipeg, MB, R3E0T6, Canada

Authors and Affiliations

  1. Department of Pharmacology, Health Science Center, University of Tennessee, Memphis, USA

    Yu-Feng Xie & FuMing Zhou

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  1. Yu-Feng Xie
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  2. FuMing Zhou
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Correspondence to Yu-Feng Xie.

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Xie, YF., Zhou, F. TRPC3 channel mediates excitation of striatal cholinergic interneurons. Neurol Sci 35, 1757–1761 (2014). https://doi.org/10.1007/s10072-014-1827-0

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  • DOI: https://doi.org/10.1007/s10072-014-1827-0

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