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Cinepazide maleate protects PC12 cells against oxygen–glucose deprivation-induced injury

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Abstract

Our previous study showed that cinepazide maleate (CM) was as effective and safe as mildronate in the treatment of acute ischemic stroke in a randomized, double-blind, active-controlled phase II multicenter trial, but underlying mechanism(s) is not well understood. As an extending study, here we demonstrated that CM could protect neuronal cells by affecting mitochondrial functions. PC12 cells were exposed to 2.5 h oxygen–glucose deprivation (OGD) followed by a 24 h reoxygenation, and then treated with different concentrations (1, 10, 100 μM) of CM. Among various concentrations, 10 μM CM exhibited most significant protection on PC12 cells against OGD injury. CM was found to suppress OGD-induced oxidative stress, as supported by its capability of reducing intracellular reactive oxygen species and malondialdehyde production and enhancing superoxide dismutase activity. Importantly, our results showed that CM could preserve mitochondrial functions, as revealed by its capability of stabilizing mitochondrial membrane potential, improving OGD-induced suppression of mitochondrial respiratory complex activities and enhancing ATP production. In summary, our present study provides the first evidence that CM can protect neuronal cells against OGD injury by preserving mitochondrial functions.

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Acknowledgments

We thank Ms. Dongyun Feng and Ms. Rui Wu for the technique support. This work was supported by the National Natural Science Foundation of China (Nos. 31170801 and 81371365) and by Program for Changjiang Scholars and Innovative Research Team in University (No. IRT1053).

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Authors and Affiliations

  1. Department of Neurology, Xijing Hospital, Fourth Military Medical University, No. 15 Changle-Xi Road, Xi’an, 710032, China

    Jun Zhao, Ya Bai, Chen Zhang, Xiao Zhang, Yun-Xia Zhang, Jing Chen, Ming Shi & Gang Zhao

  2. Department of Neurology, Second Artillery General Hospital of PLA, Beijing, 100088, China

    Chen Zhang

  3. Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, China

    Lize Xiong

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  1. Jun Zhao
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  2. Ya Bai
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  3. Chen Zhang
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  4. Xiao Zhang
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  5. Yun-Xia Zhang
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  6. Jing Chen
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  7. Lize Xiong
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  8. Ming Shi
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  9. Gang Zhao
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Correspondence to Ming Shi or Gang Zhao.

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J. Zhao and Y. Bai contributed equally to this work.

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Zhao, J., Bai, Y., Zhang, C. et al. Cinepazide maleate protects PC12 cells against oxygen–glucose deprivation-induced injury. Neurol Sci 35, 875–881 (2014). https://doi.org/10.1007/s10072-013-1618-z

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  • DOI: https://doi.org/10.1007/s10072-013-1618-z

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