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A model of synaptic plasticity: activation of mGluR I induced long-term theta oscillations in medial septal diagonal band of rat brain slice

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Abstract

This study aimed to establish a model of synaptic plasticity by the activation of metabotropic glutamate receptor (mGluR) I in rat medial septal diagonal band (MSDB). Electrophysiological experiment was performed to record the theta frequency oscillation activities in rat MSDB slices. The data were recorded and analyzed with Spike 2 (CED, Cambridge, UK). Application of aminocyclopentane-1, 3-dicarboxylic acid (ACPD) to MSDB slices produced theta frequency oscillations (4–12 Hz) which persisted for hours after ACPD washout, suggesting the existence of a form of synaptic plasticity in long-term oscillations (LTOs). Addition of NMDA receptor antagonist AP5 (50 μM) caused no significant change in area power. In contrast, AMPA/Kainate receptor antagonist NBQX administration partially reduced the area power. Infusion of ZD7288, a hyperpolarization-activated channel (Ih) inhibitor, caused additional reduction to control level. Comparable effects were also observed with administration of DHPG (3, 5-dihydroxyphenylglycine) which also elicited LTOs. mGluR I activation induced theta oscillation and this activity maintained hours after drug washout. Both AMPA and hyperpolarization-activated channel make an essential contribution to LTO. Our study herein established a model of synaptic plasticity.

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Acknowledgments

This work was supported by National Natural Foundation of China (grant nos. 31070938; 81271422) and the UK Medical Research Council (grant nos. G0500823).

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Authors and Affiliations

  1. Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, Henan Province, People’s Republic of China

    Chengzhang Li, Zhiyue Guo, Yali Wang, Xiaojuan Li & Cheng B. Lu

  2. Faculty of Biological Sciences, Institute of Membrane and Systems Biology, University of Leeds, Leeds, LS2 9JT, UK

    Zainb Henderson

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  1. Chengzhang Li
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  2. Zhiyue Guo
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Correspondence to Cheng B. Lu.

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Li, C., Guo, Z., Wang, Y. et al. A model of synaptic plasticity: activation of mGluR I induced long-term theta oscillations in medial septal diagonal band of rat brain slice. Neurol Sci 35, 551–557 (2014). https://doi.org/10.1007/s10072-013-1543-1

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