Abstract
Down’s syndrome (DS) is the most frequent genetic cause of intellectual disability and is a chromosomal abnormality of chromosome 21 trisomy. The pericentrin gene (PCNT) has sequenced in 21q22.3 inside of the minimal critical region for Down’s syndrome. Alterations of PCNT gene are associated with dwarfism, cardiomyopathy and other pathologies. In this study, we have evaluated the possible differential expression of PCNT mRNA, by qRT-PCR, in peripheral blood leukocytes of DS subjects compared with the normal population. In the present case–control study, PCNT gene expression was increased by 72.72 % in 16 out 22 DS samples compared with normal subjects. Our data suggest that changes in the expression levels of PCNT in DS subjects may be involved into the molecular mechanism of Down’s syndrome.
References
Capone GT (2001) Down syndrome: advances in molecular biology and the neurosciences. J Dev Behav Pediatr 22:40–59
Rauch A (2011) The shortest of the short: pericentrin mutations and beyond. Best Pract Res Clin Endocrinol Metab 25:125–130
Flory MR, Moser MJ, Monnat RJ Jr, Davis TN (2000) Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin. Proc Natl Acad Sci USA 97:5919–5923
Doxsey S, Zimmerman W, Mikule K (2005) Centrosome control of the cell cycle. Trends Cell Biol 15:303–311
Zimmerman WC, Sillibourne J, Rosa J, Doxsey SJ (2004) Mitosis-specific anchoring of gamma tubulin complexes by pericentrin controls spindle organization and mitotic entry. Mol Biol Cell 15:3642–3657
Salemi M, Barone C, Romano C, Zolezzi F, Romano C, Scavuzzo C, Salluzzo R, Scillato F, Signorelli M, Kapetis D, Salluzzo MG, Bosco P (2012) Gene expression profiling and qRT-PCR expression of RRP1B, PCNT, KIF21A and ADRB2 in leucocytes of Down’s syndrome subjects. J Genet 91:e18–e23
Salemi M, Barone C, Romano C, Ridolfo F, Scavuzzo C, Cantarella RA, Salluzzo MG, Calogero AE, Romano C, Bosco P (2013) KIF21A mRNA expression in patients with Down syndrome. Neurol Sci 34:569–571
Salemi M, Longo GA, La Vignera S, Romano C, Condorelli RA, Romano C, Bosco P, Vicari E, Calogero AE (2013) SPAG5 mRNA is over-expressed in peripheral blood leukocytes of patients with Down’s syndrome and cryptorchidism. Neurol Sci 34:549–551
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C (T)). Method 4:402–408
Lorenzo LP, Shatynski KE, Clark S, Yarowsky PJ, Williams MS (2013) Defective thymic progenitor development and mature T-cell responses in a mouse model for Down syndrome. Immunology 139:447–458
Wilcock DM, Griffin WS (2013) Down’s syndrome, neuroinflammation, and Alzheimer neuropathogenesis. J Neuroinflamm 10:84
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Salemi, M., Barone, C., Romano, C. et al. Pericentrin expression in Down’s syndrome. Neurol Sci 34, 2023–2025 (2013). https://doi.org/10.1007/s10072-013-1529-z
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DOI: https://doi.org/10.1007/s10072-013-1529-z