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Expression of Bcl-2 and Bax after hippocampal ischemia in DHA + EPA treated rats

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Abstract

To determine the impact of ω3 fatty acids on post-ischemic expression of pro- and anti-apoptotic proteins in hippocampus, male rats were received 10 or 100 mg/kg [Docosahexaenoic acid (DHA) + Ecosapentaenoic acid (EPA); gavage; 21 days before ischemia to 2–10 days after ischemia]. Global cerebral ischemia reperfusion (IR) was performed using the four-vessel occlusion model; ischemia 8 min and reperfusion 6, 48 h and 10 days. IR increased Bcl-2 and Bax expression after 48 h (p < 0.05 and p < 0.01 vs. sham) and 10 days (only Bax; p < 0.05), without significant difference with DHA + EPA groups after 6 h. But after 48 h expression of Bcl-2 increased (p < 0.05 vs. IR) and Bax decreased (p < 0.05). At day 10 after ischemia expression of Bax in DHA + EPA acid groups was less than IR (p < 0.05) and in 100 mg/kg DHA + EPA group Bcl-2 expression was more than IR (p < 0.05). These data suggested that long-term gavage with DHA + EPA increase hippocampal neurons survival for days after ischemia, revealed by increased Bcl-2 and decreased Bax expressions.

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Acknowledgments

The authors appreciate Shahid Beheshti University of Medical Sciences for supporting equipments and measurements.

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Authors and Affiliations

  1. Physiology Research Center, Tehran University of Medical Sciences, PO BOX 14515-763, Tehran, Iran

    Marjan Ajami, Rouhollah Habibey & Hamidreza Pazoki-Toroudi

  2. Department of Nutrition, Tehran University of Medical Sciences, Tehran, Iran

    Marjan Ajami & Shariar Eghtesadi

  3. Nano Medicine and Tissue Engineering Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Jalaledin Mirzay Razaz & Naser Kalantari

  4. Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Ali Nilforoushzadeh

  5. Skin Diseases and Leishmaniasis Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran

    Mohammad Ali Nilforoushzadeh

  6. Department of Pharmacology, Tehran University of Medical Science, Tehran, Iran

    Mohammadreza Zarrindast

  7. Nano Vichar Pharmaceutical Ltd, Tehran, Iran sciences, Tehran, Iran

    Hamidreza Pazoki-Toroudi

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  1. Marjan Ajami
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Correspondence to Hamidreza Pazoki-Toroudi.

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Ajami, M., Eghtesadi, S., Razaz, J.M. et al. Expression of Bcl-2 and Bax after hippocampal ischemia in DHA + EPA treated rats. Neurol Sci 32, 811–818 (2011). https://doi.org/10.1007/s10072-011-0621-5

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  • DOI: https://doi.org/10.1007/s10072-011-0621-5

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