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Changes in the anxiety-like and fearful behavior of shrimp following daily threatening experiences

Abstract

Behavioral variation in animals is often influenced by experience. Previous studies have found that daily threatening experiences can enhance fear- and anxiety-like behaviors in some vertebrates. However, it is unclear whether the change in fear/anxiety behavior occurs in invertebrates. The present study investigated whether fear/anxiety behavior could be affected by a net-chasing treatment in two shrimp species (Neocaridina denticulata ssp. and Palaemon pacificus). The net-chasing treatment was repeated for 8 days to simulate daily predator experiences, and behavioral tests (open-field, shelter-seeking, and escape-response tests) were conducted on the day following the last day of treatment. Net-chased N. denticulata ssp. displayed a tendency to remain near a wall compared with the control in the open-field test, whereas net-chased P. pacificus shrimps demonstrated greater escape behavior compared with the control in the escape-response test. These results suggest that fear/anxiety behavior for both shrimp species can be affected by the net-chasing treatment, although the pattern of behavioral change differed between the two species. The findings suggest that daily threatening experiences change the behavior of shrimp and cause them to select a regular avoidance strategy when they encounter risks and unknown situations.

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Data availability

The data of each individual are uploaded in Supplemental file.

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Acknowledgements

The present study was funded by the Grant-in-Aid for JSPS Fellows (JSPS KAKENHI Grant I thank Dr. Takeshi Takegaki and the students of the Evolutionary and Behavioral Ecology Laboratory, Nagasaki University for providing support to conduct this experiment.

Funding

The present study was funded by the Grant-in-Aid for JSPS Fellows (JSPS KAKENHI Grant Numbers 15J06124 and 18K14512).

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Correspondence to Kohji Takahashi.

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This study was approved by the Animal Care and Use Committee of the Faculty of Fisheries, Nagasaki University (permission no. NF-0005), in accordance with the Guidelines for Animal Experimentation of the Faculty of Fisheries (fish, amphibians and invertebrates).

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Takahashi, K. Changes in the anxiety-like and fearful behavior of shrimp following daily threatening experiences. Anim Cogn (2021). https://doi.org/10.1007/s10071-021-01555-8

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Keywords

  • Avoidance
  • Decapod
  • Neocaridina denticulate
  • Non-associative learning
  • Palaemon pacificus
  • Training