Abstract
Aqueous enzyme-assisted solvent extraction (AE-SE) of oil from apricot (Prunus armeniaca L.) kernel was investigated in this study and the operational parameters were optimized. After preliminary screening, a cocktail of enzymes (Celluclast 1.5 L and Alcalase Pure 2.4 L [60:40, v/v)] of 1% (v/w) was chosen. The extraction process parameters: temperature (40–60 °C), time (1–5 h), and pH (4–9) were optimized using Box–Behnken design to achieve the highest oil yield (%) and antioxidant activity (DPPH, %). Under optimized conditions, i.e., temperature 40 °C, time 2.5 h, and pH 8.28, the highest oil yield and DPPH were 47.93% and 67.31%, respectively. The gas chromatography analysis disclosed that apricot kernel oil extracted by solvent extraction and AE-SE have similar fatty acid compositions, and the oil is rich in unsaturated fatty acids. The physicochemical analysis showed AE-SE method produces high-quality oil and can be substituted as green technology for industrial oil extraction purposes.
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Akhone MA, Bains A, Tosif MM, Chawla P, Fogarasi M, Fogarasi S. Apricot kernel: bioactivity, characterization, applications, and health attributes. Foods 11: 2184 (2022)
Akinoso R, Raji AO. Optimization of oil extraction from locust bean using response surface methodology. European Journal of Lipid Science and Technology 113: 245-252 (2011)
AOAC. Official Methods of Analysis of AOAC International. AOAC International, Gaithersburg, USA (2005)
AOCS. Official Methods and Recommended Practices of the American Oil Chemists’ Society. AOCS Press, Champaign, USA (1997)
Balvardi M, Rezaei K, Mendiola JA, Ibáñez E. Optimization of the aqueous enzymatic extraction of oil from Iranian wild almond. Journal of the American Oil Chemists' Society 92: 985-992 (2015)
TS, Sharma SK, Sati RC, Rao VK, Yadav VK, Dixit AK, Sharma AK, Chopra CS. Improvement of efficiency of oil extraction from wild apricot kernels by using enzymes. Journal of Food Science and Technology 52: 1543-1551 (2013)
Cheng M, Rosentrater KA, Sekhon J, Wang T, Jung S, Johnson LA. Economic feasibility of soybean oil production by enzyme-assisted aqueous extraction processing. Food and Bioprocess Technology 12: 539-550 (2019)
Fang X, Fei X, Sun H, Jin Y. Aqueous enzymatic extraction and demulsification of camellia seed oil (Camellia oleifera Abel.) and the oil’s physicochemical properties. European Journal of Lipid Science and Technology 118: 244-251 (2016)
Gai QY, Jiao J, Wei FY, Luo M, Wang W, Zu YG, Fu YJ. Enzyme-assisted aqueous extraction of oil from Forsythia suspense seed and its physicochemical property and antioxidant activity. Industrial Crops and Products 51: 274-278 (2013)
Hu B, Zhou K, Liu Y, Liu A, Zhang Q, Han G, Liu S, Yang Y, Zhu Y, Zhu D. Optimization of microwave-assisted extraction of oil from tiger nut (Cyperus esculentus L.) and its quality evaluation. Industrial Crops and Products 115: 290-297 (2018)
Jiao J, Li ZG, Gai QY, Li XJ, Wei FY, Fu YJ, Ma W. Microwave-assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical properties, fatty acid compositions and antioxidant activities. Food Chemistry 147: 17-24 (2014)
Juhaimi FAL, Özcan MM, Ghafoor K, Babiker EE. The effect of microwave roasting on bioactive compounds, antioxidant activity, and fatty acid composition of apricot kernel and oils. Food Chemistry 243: 414-419 (2018)
Jung S, Maurer D, Johnson LA. Factors affecting emulsion stability and quality of oil recovered from enzyme-assisted aqueous extraction of soybeans. Bioresource Technology 100: 5340-5347 (2009)
Kim SM, Lim ST. Enhanced antioxidant activity of rice bran extract by carbohydrase treatment. Journal of Cereal Science 68: 116-121 (2016)
Liu JJ, Gasmalla MAA, Li P, Yang R. Enzyme-assisted extraction processing from oilseeds: principle, processing, and application. Innovative Food Science and Emerging Technologies 35: 184-193 (2016)
Liu Z, Gui M, Xu T, Zhang L, Kong L, Qin L, Zou Z. Efficient aqueous enzymatic-ultrasonication extraction of oil from Sapindus mukorossi seed kernels. Industrial Crops and Products 134: 124-133 (2019)
Mahatre RR, Kumbhar BK, Singh A, Lohani UC, Shahi NC. Optimization of parameters for enhanced oil recovery from enzyme-treated wild apricot kernels. Journal of Food Science and Technology 49: 482-488 (2012)
Miron TL, Herrero M, Ibáñez E. Enrichment of antioxidant compounds from lemon balm (Melissa officinalis) by pressurized liquid extraction and enzyme-assisted extraction. Journal of Chromatography A 1288: 1-9 (2013)
Nadar SS, Rao P, Rathod VK. Enzyme assisted extraction of biomolecules as an approach to novel extraction technology: a review. Food Research International 108: 309-330 (2018)
Pawar KR, Nema PK. Apricot kernel characterization, oil extraction, and its utilization: a review. Food Science and Biotechnology 32: 249-236 (2023)
Pawar KR, Nema PK, Babar OA, Yadav DK. Effect of moisture content on engineering characteristics of apricot (Prunus armeniaca L.) kernel. Journal of Food Process Engineering 46: 1-11 (2023)
Ramadan MF, Sitohy MZ, Moersel JT. Solvent and enzyme-aided aqueous extraction of goldenberry (Physalis peruviana L.) pomace oil: impact of processing on composition and quality of oil and meal. European Food Research and Technology 226: 1445-1458 (2008)
Rezvankhah A, Emmam-Djomeh Z, Safari M, Askari G, Salami M. Microwave-assisted extraction of hempseed oil: studying and comparing of fatty acid composition, antioxidant activity, physiochemical and thermal properties with Soxhlet extraction. Journal of Food Science and Technology 56: 4198-4210 (2019)
Ribeiro SAO, Nicacio AE, Zanqui AB, Biondo PBF, de Abreu-Filho BA, Visentainer JV, Gomes STM, Matsushita M. Improvements in the quality of sesame oil obtained by a green extraction method using enzymes. LWT-Food Science and Technology 65: 464-470 (2016)
Rovaris ÂA, Dias CO, da Cunha IP, Scaff RMC, de Francisco A, Petkowicz CLO, Amante ER. Chemical composition of solid waste and effect of enzymatic oil extraction on the microstructure of soybean (Glycine max). Industrial Crops and Products 36: 405-414 (2012)
Song Y, Zhang W, Wu J, Admassu H, Liu J, Zhao W, Yang R. Ethanol-assisted aqueous enzymatic extraction of peony seed oil. Journal of the American Oil Chemists' Society 96: 595-606 (2019)
Stryjecka M, Kiełtyka-Dadasiewicz A, Michalak M, Rachoń L, Głowacka A. Chemical composition and antioxidant properties of oils from the seeds of five apricot (Prunus armeniaca L.) cultivars. Journal of Oleo Science 68: 729-738 (2019)
Yolmeh M, Habibi Najafi MB, Farhoosh R. Optimisation of ultrasound-assisted extraction of natural pigment from annatto seeds by response surface methodology (RSM). Food Chemistry 155: 319-324 (2014)
Zhang YL, Li S, Yin CP, Jiang DH, Yan FF, Xu T. Response surface optimisation of aqueous enzymatic oil extraction from bayberry (Myrica rubra) kernels. Food Chemistry 135: 304-308 (2012)
Zhou B, Sun Y, Li J, Long Q, Zhong H. Effects of seed coat on oxidative stability and antioxidant activity of apricot (Prunus armeniaca L.) kernel oil at different roasting temperatures. Journal of the American Oil Chemists' Society 95: 1297-1306 (2018)
Acknowledgements
The authors would like to extend their sincere appreciation to the National Institute of Food Technology Entrepreneurship and Management-Kundli, Dist Sonepat, State Haryana, India for supporting and providing lab and library facilities to conduct smooth research. The authors sincerely acknowledge Novozymes for their generous donation of enzymes for research purposes.
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Pawar, K.R., Nema, P.K., Gupta, R. et al. Optimization and characterization of aqueous enzyme-assisted solvent extraction of apricot kernel oil. Food Sci Biotechnol (2024). https://doi.org/10.1007/s10068-024-01553-9
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DOI: https://doi.org/10.1007/s10068-024-01553-9