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Quality evaluation and storage test for capsaicin-fortified yogurt based on the multilayer nanoemulsion system

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Abstract

Capsaicin has many benefits, such as pain relief, cancer prevention, and weight reduction. However, the application of capsaicin has been limited in the food industry due to its strong pungency, odor, and low solubility in water. Therefore, a multilayer nanoemulsion with chitosan and hyaluronic acid was developed for masking its odor and taste and improving the physicochemical stability against the surrounding environment. The capsaicin-fortified yogurts were prepared by blending various concentration levels of multilayer nanoemulsion (0–15%, w/v). The quality of yogurt was determined as a function of pH, acidity, viscosity, and total lactic acid bacteria population in an extended storage period (21 days). The multivariate statistical analysis was used to compare the quality of yogurts supplemented with capsaicin nanoemulsion. As a result, this study demonstrated the potential of capsaicin-loaded multilayer emulsion-supplemented yogurt as a novel nutrition-fortified food.

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References

  • Akbas E, Soyler B, Oztop MH. Capsaicin emulsions: Formulation and characterization. Journal of Dispersion Science and Technology. 38: 1079-1086 (2016)

    Article  Google Scholar 

  • Alfaro L, Hayes D, Boeneke C, Xu Z, Bankston D, Bechtel PJ, Sathivel S. Physical properties of a frozen yogurt fortified with a nano-emulsion containing purple rice bran oil. LWT-Food Science and Technology. 62(2): 1184-1191 (2015)

    Article  CAS  Google Scholar 

  • Anthero AGS, Moya AMTM, Torsoni AS, Cazarin CB, Hubinger MD. Characterization of Capsicum oleoresin microparticles and in vivo evaluation of short-term capsaicin intake. Food Chemistry: X. 13: 100179 (2022)

    Google Scholar 

  • Askin B, Öztürk D, Durusoy B, Kaya Y. Properties of yogurt fortified with capsaicin extracts dissolved in high oleic sunflower oil. Journal of Food Processing and Preservation. e15633 (2021)

    Article  Google Scholar 

  • Badran MM, Taha EI, Tayel MM, Al-Suwayeh SA. Ultra-fine self nanoemulsifying drug delivery system for transdermal delivery of meloxicam: dependency on the type of surfactants. Journal of Molecular Liquids. 190: 16-22 (2014)

    Article  CAS  Google Scholar 

  • Beal C, Skokanova J, Latrille E, Martin N, Corrieu G. Combined effects of culture conditions and storage time on acidification and viscosity of stirred yogurt. Journal of Dairy Science. 82: 673-681 (1999)

    Article  CAS  Google Scholar 

  • Chang SH, Lin HTV, Wu GJ, Tsai GJ. pH Effects on solubility, zeta potential, and correlation between antibacterial activity and molecular weight of chitosan. Carbohydrate Polymers. 134: 74-81 (2015)

    Article  CAS  PubMed  Google Scholar 

  • Choi AJ, Kim CJ, Cho YJ, Hwang JK, Kim CT. Characterization of capsaicin-loaded nanoemulsions stabilized with alginate and chitosan by self-assembly. Food and Bioprocess Technology. 4(6): 1119-1126 (2011)

    Article  CAS  Google Scholar 

  • Codex Alimentarius Commission. Codex standard for fermented milks. Codex Stan 243–2003 (2015)

  • Damin MR, Minowa E, Alcantara MR, Oliveira MN. Effect of cold storage on culture viability and some rheological properties of fermented milk prepared with yogurt and probiotic bacteria. Journal of Texture Studies. 39: 40-55 (2008)

    Article  Google Scholar 

  • Erasmus SW, Muller M, Butler M, Hoffman LC. The truth is in the isotopes: Authenticating regionally unique South African lamb. Food Chemistry. 239: 926–934 (2018)

    Article  CAS  PubMed  Google Scholar 

  • Fardous J, Omoso Y, Joshi A, Yoshida K, Patwary MKA, Ono F, Ijima H. Development and characterization of gel-in-water nanoemulsion as a novel drug delivery system. Materials Science and Engineering: C. 124: 112076 (2021)

    Article  CAS  PubMed  Google Scholar 

  • Garavand F, Rouhi M, Razavi SH, Cacciotti I, Mohammadi R. Improving the integrity of natural biopolymer films used in food packaging by crosslinking approach: A review. International Journal of Biological Macromolecules. 104: 687-707 (2017)

    Article  CAS  PubMed  Google Scholar 

  • Granato D, Santos JS, Escher GB, Ferreira BL, Maggio RM. Use of principal component analysis (PCA) and hierarchical cluster analysis (HCA) for multivariate association between bioactive compounds and functional properties in foods: A critical perspective. Trends in Food Science & Technology. 72: 83-90 (2018)

    Article  CAS  Google Scholar 

  • Gumus CE, Gharibzahedi SMT. Yogurts supplemented with lipid emulsions rich in omega-3 fatty acids: New insights into the fortification, microencapsulation, quality properties, and health-promoting effects. Trends in Food Science & Technology. 110: 267-279 (2021)

    Article  CAS  Google Scholar 

  • Guner A, Ardıc M, Keles A, Dogruer Y. Production of yogurt ice cream at different acidity. International Journal of Food Science & Technology. 42: 948-952 (2007)

    Article  CAS  Google Scholar 

  • Hong IK, Kim SI, Lee SB. Effects of HLB value on oil-in-water emulsions: Droplet size, rheological behavior, zeta-potential, and creaming index. Journal of Industrial and Engineering Chemistry. 67: 123-131 (2018)

    Article  CAS  Google Scholar 

  • Isleten M, Karagul-Yuceer YONCA. Effects of dried dairy ingredients on physical and sensory properties of nonfat yogurt. Journal of Dairy Science. 89: 2865-2872 (2006)

    Article  CAS  PubMed  Google Scholar 

  • Jandrić Z, Cannavan A. An investigative study on differentiation of citrus fruit/fruit juices by UPLC-QToF MS and chemometrics. Food Control. 72: 173-180 (2017)

    Article  Google Scholar 

  • Kim JH, Ko JA, Kim JT, Cha DS, Cho JH, Park HJ, Shin GH. Preparation of a capsaicin-loaded nanoemulsion for improving skin penetration. Journal of Agricultural and Food Chemistry. 62: 725-732 (2014)

    Article  CAS  PubMed  Google Scholar 

  • Kim SH, Lim CH, Lee C, An G. Optimization of growth and storage conditions for lactic acid bacteria in yogurt and frozen yogurt. Journal of the Korean Society for Applied Biological Chemistry. 52: 76-79 (2009)

    Article  CAS  Google Scholar 

  • Kong M, Park HJ. Stability investigation of hyaluronic acid based nanoemulsion and its potential as transdermal carrier. Carbohydrate Polymers. 83: 1303-1310 (2011)

    Article  CAS  Google Scholar 

  • Li R, Lan Y, Chen C, Cao Y, Huang Q, Ho CT, Lu M. Anti-obesity effects of capsaicin and the underlying mechanisms: a review. Food & Function. 11: 7356-7370 (2020)

    Article  CAS  Google Scholar 

  • Luesakul U, Puthong S, Sansanaphongpricha K, Muangsin N. Quaternized chitosan-coated nanoemulsions: A novel platform for improving the stability, anti-inflammatory, anti-cancer and transdermal properties of Plai extract. Carbohydrate Polymers. 230: 115625 (2020)

    Article  CAS  PubMed  Google Scholar 

  • Lund JA, Brown PN, Shipley PR. Differentiation of Crataegus spp. guided by nuclear magnetic resonance spectrometry with chemometric analyses. Phytochemistry. 141: 11-19 (2017)

    Article  CAS  PubMed  Google Scholar 

  • Machado TADG, de Oliveira MEG, Campos MIF, de Assis POA., de Souza EL, Madruga MS, Pacheco MTB, Pintado MME, do Egypto RDCR. Impact of honey on quality characteristics of goat yogurt containing probiotic Lactobacillus acidophilus. LWT-Food Science and Technology. 80: 221-229 (2017)

    Article  CAS  Google Scholar 

  • Mahmoodi Pour H, Marhamatizadeh MH, Fattahi H. Encapsulation of different types of probiotic bacteria within conventional/multilayer emulsion and its effect on the properties of probiotic yogurt. Journal of Food Quality. 2022: 1-12 (2022)

    Article  Google Scholar 

  • Pathakoti K, Manubolu M, Hwang HM. Nanostructures: Current uses and future applications in food science. Journal of Food and Drug Analysis. 25: 245-253 (2017)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Qin H, Huo D, Zhang L, Yang L, Zhang S, Yang M, Shen C, Hou C. Colorimetric artificial nose for identification of Chinese liquor with different geographic origins. Food Research International. 45: 45-51 (2012)

    Article  Google Scholar 

  • Silva HD, Poejo J, Pinheiro AC, Donsì F, Serra AT, Duarte CM, Ferrari G, Cerqueira MA, Vicente AA. Evaluating the behaviour of curcumin nanoemulsions and multilayer nanoemulsions during dynamic in vitro digestion. Journal of Functional Foods. 48: 605-613 (2018)

    Article  CAS  Google Scholar 

  • Sun J, Perry SL, Schiffman JD. Electrospinning nanofibers from chitosan/hyaluronic acid complex coacervates. Biomacromolecules. 20: 4191-4198 (2019)

    Article  CAS  PubMed  Google Scholar 

  • Tamjidi F, Nasirpour A, Shahedi M. Physicochemical and sensory properties of yogurt enriched with microencapsulated fish oil. Food Science and Technology International. 18: 381-390 (2012)

    Article  CAS  PubMed  Google Scholar 

  • Vignesh S, Sivashanmugam A, Annapoorna M, Janarthanan R, Subramania I, Jayakumar R. Injectable deferoxamine nanoparticles loaded chitosan-hyaluronic acid coacervate hydrogel for therapeutic angiogenesis. Colloids and Surfaces B: Biointerfaces. 161: 129-138 (2018)

    Article  CAS  Google Scholar 

  • Yang E, Fan L, Jiang Y, Doucette C, Fillmore S. Antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from cheeses and yogurts. Amb Express. 2(1): 1-12 (2012)

    Article  Google Scholar 

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (grant of NRF-2016R1D1A1B03933896) and by Institute of Biomedical Science & Food Safety, Korea University, Republic of Korea.

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JAK—Conceptualization, methodology, validation, formal analysis, investigation, resources, data curation, JK—Conceptualization, methodology, validation, formal analysis, investigation, resources, HD—Data curation, writing—Original Draft, writing—Review & Editing, HJP—Supervision, project administration, funding acquisition.

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Correspondence to Hansol Doh or Hyun Jin Park.

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Ko, J.A., Kim, J., Doh, H. et al. Quality evaluation and storage test for capsaicin-fortified yogurt based on the multilayer nanoemulsion system. Food Sci Biotechnol 33, 441–451 (2024). https://doi.org/10.1007/s10068-023-01386-y

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  • DOI: https://doi.org/10.1007/s10068-023-01386-y

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