Abstract
In this study, gas chromatography–mass spectrometry (GC–MS) coupled with Partial least squares Discriminant Analysis (PLS-DA) was used to analyze the volatiles in Chinese breast milk from different cities (Wuhan, Qingdao and Hohhot) and different lactation stages (colostrum and mature milk). The results showed that breast milk contained 122 volatile substances in 9 major groups, with the largest number of olefins (36) and the highest content of acids. The different volatile compounds of breast milk in three cities were heptanal, 2-pentylfuran, (E)-2,4-decadienal, (E)-2-heptenal, (E)-2-nonenal and 1-octen-3-one, colostrum and mature milk were (E)-2-heptenal, (E)-2-decenal, lauric acid, n-decanoic acid, (E)-2-nonenal and octanoic acid. This study might provide scientific data for the development and optimization of formulas that were more suitable for the health of Chinese infants.
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Campanhon IB, Da Silva MRS, de Magalhães MTQ, Zingali RB, Bezerra FF, Torres AG. Protective factors in mature human milk: a look into the proteome and peptidome of adolescent mothers’ breast milk. British Journal of Nutrition. 122: 1377-1385 (2019)
Contador R, Delgado FJ, García-Parra J, Garrido M, Ramírez R. Volatile profile of breast milk subjected to high-pressure processing or thermal treatment. Food Chemistry. 180: 17-24 (2015)
Demmelmair H, Koletzko B. Lipids in human milk. Best Practice & Research Clinical Endocrinology & Metabolism. 32: 57-68 (2018)
Hausner H, Philipsen M, Skov TH, Petersen MA, Bredie WLP. Characterization of the volatile composition and variations between infant formulas and mother’s milk. Chemosensory Perception. 2: 79-93 (2009)
He Y, Chen L, Zheng L, Cheng F, Deng Z, Luo T, Li J. A comparative study of volatile compounds in breast milk and infant formula from different brands, countries of origin, and stages. European Food Research and Technology. 248: 2679-2694 (2022)
Hu S, Wei H, Guo S, Li L, Hou Y. Flavor evaluation of yak butter in Tsinghai-Tibet Plateau and isolation of microorganisms contributing flavor. Animal Science Journal. 82: 122-126 (2011)
Huang Z, Hu Y. Dietary patterns and their association with breast milk macronutrient composition among lactating women. International Breastfeeding Journal. 15: 52 (2020)
Jiang M. Effect of ultra-high pressure homogenization and heat treatment on the characteristics of soy milk, tofu and bean curd skin. Nanjing Agricultural University, Nanjing (2013)
Kim SY, Yi DY. Components of human breast milk: from macronutrient to microbiome and microRNA. Clinical and Experimental Pediatrics. 63: 301-309 (2020)
Li J, Fan Y, Zhang Z, Yu H, An Y, Kramer JKG, Deng Z. Evaluating the trans fatty acid, CLA, PUFA and erucic acid diversity in human milk from five regions in China. Lipids. 44: 257-271 (2009)
Liu X. Study on the role of cinnamon in the flavor formation of marinated chicken. Henan Agricultural University, Zhengzhou (2013)
Liu D. Analysis of volatile components of oats and oatmeal and the effect of oatmeal on patients with hyperlipidemia. Northwest Agriculture and Forestry University of Science and Technology (2014)
Liu R. Study on the effects of soybean varieties and pulping process on undesirable flavor substances in soybean milk. Jilin Agricultural University, Changchun (2016)
Shimoda M, Yoshimura T, Ishikawa H, Hayakawa I, Osajima Y. Volatile compounds of human milk. Journal of the Faculty of Agriculture, Kyushu University. 45: 199-206 (2000)
Spitzer J, Buettner A. Characterization of aroma changes in human milk during storage at −19°C. Food Chemistry. 120: 240-246 (2010)
Wang Y. Identification of characteristic flavour substances and main influencing factors of podded beans. Jilin Agricultural University, Changchun (2015)
Wang J, Yang ZJ, Xu LY, Wang B, Zhang JH, Li BZ, Cao YP, Tan L. Key aroma compounds identified in Cheddar cheese with different ripening times by aroma extract dilution analysis, odor activity value, aroma recombination, and omission. Journal of Dairy Science. 104: 1576-1590 (2021)
Zhang Y, Xia Y, Luo F. Determination of volatile flavor components in duck soup by solid-phase microextraction-gas chromatography. Food Science. 33: 225-231 (2012)
Zhang T-T, Zeng MF, Jin Y, Liu L-Y. Determination of nucleosides and nucleotides in breast milk and milk powder by reversed-phase high performance liquid chromatography-tandem mass spectrometry. Analytical Chemistry. 42: 7 (2014)
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This research work was supported by the Department of Science and Technology of Jiangxi Province (20212BBF63033), China.
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He, Y., Chen, L., Liu, W. et al. Comparative analysis of the volatile components in Chinese breast milk from three regions. Food Sci Biotechnol 32, 903–909 (2023). https://doi.org/10.1007/s10068-022-01235-4
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DOI: https://doi.org/10.1007/s10068-022-01235-4