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The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides extracted from Lentinula edodes

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Abstract

In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated. The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose. Moreover, γ-irradiation treatment caused LEP color changes and surface topography destroyed. γ-Irradiated LEP showed higher hypoglycemic activities in vitro than that of non-irradiated LEP. Moreover, γ-irradiated LEP had better proliferation promoting effects on Lactobacillus rhamnosus and L. plantarum. These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (No. 31801921 & 32072229). Young-aged Top-notch Talent Training Program of Hubei Academy of Agricultural Sciences (2022). Special Project for Science and Technology Innovation of Wuhan (2022020801010343). Project of Edible Fungus Industrial Technology System of Hubei Province (HBHZDZB-2021-023).

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Authors and Affiliations

  1. National Research and Development Center for Edible Fungi Processing (Wuhan), Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, 430064, China

    Chaomin Yin, Chen Li, Kun Ma, Xiuzhi Fan, Fen Yao, Defang Shi, Wenjing Wu, Jianhui Qiu & Hong Gao

  2. School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, 430205, China

    Chen Li & Guoyuan Hu

  3. Research Center of Under-Forest Economy in Hubei Province, Wuhan, 430064, China

    Hong Gao

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  1. Chaomin Yin
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Fig. S1

HPLC spectra of monosaccharide composition. a: standard monosaccharide; b: LEP0; C: LEP4; d: LEP8; e: LEP16. (1-mannose, 2-ribose, 3-rhamnose, 4-glucuronic acid, 5-glucose, 6-xylose, 7-galacturonic acid, 8-galactose, 9-arabinose and 10-fucose). (TIF 5458 kb)

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Yin, C., Li, C., Ma, K. et al. The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides extracted from Lentinula edodes. Food Sci Biotechnol 32, 987–996 (2023). https://doi.org/10.1007/s10068-022-01234-5

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