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Probiotic and antioxidant properties of C30 carotenoid-producing Lactiplantibacillus plantarum isolated from kimchi

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Abstract

This study focuses on the development of functional probiotics using caroteonid-producing lactic acid bacteria (LAB) with antioxidant properties. Thirty LAB strains were evaluated for their probiotic properties. Carotenoid biosynthesis gene cluster (crtMN operon) was detected using polymer chain reaction (PCR). The carotenoid identified as 4,4′-diaponeurosporene was analyzed via UV visible absorption spectra and HPLC. Five carotenoid-producing strains showed antioxidant activities. Lactiplantibacillus plantarum MGB0112, which showed the highest carotenoid production measuring at 470 nm of absorbance per ml of culture broth (0.014 A470nm/ml), showed low pH (56.5%) and bile salt (97.8%) tolerance with high adhesion properties (55.1% for toluene). Furthermore, this strain and 4,4′-diaponeurosporene extract exhibited antioxidant activity (99.5 and 40.1%, respectively) against DPPH free radicals in vitro. Their antioxidant properties were confirmed in vivo (45.6 and 55.2% survival rates in Caenorhabditis elegans). Therefore, C30 carotenoid-producing strain MGB0112 demonstrates outstanding antioxidant effects and can be a potential functional probiotics.

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Acknowledgements

This work was supported by Incheon National University Research Grant in 2021.

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Author notes

  1. Chi Young Hwang and Eui-Sang Cho contributed equally to this work.

Authors and Affiliations

  1. Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon, 22012, Republic of Korea

    Chi Young Hwang, Eui-Sang Cho, Deok Jun Yoon & Myung-Ji Seo

  2. Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Myung-Ji Seo

  3. Research Center for Bio Materials & Process Development, Incheon National University, Incheon, 22012, Republic of Korea

    Myung-Ji Seo

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  1. Chi Young Hwang
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  2. Eui-Sang Cho
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Correspondence to Myung-Ji Seo.

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Hwang, C.Y., Cho, ES., Yoon, D.J. et al. Probiotic and antioxidant properties of C30 carotenoid-producing Lactiplantibacillus plantarum isolated from kimchi. Food Sci Biotechnol 32, 543–552 (2023). https://doi.org/10.1007/s10068-022-01226-5

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