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The effect of fermented rice germ extracts on the inhibition of glucose uptake in the gastrointestinal tract in vitro and in vivo

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Abstract

This study aimed to evaluate the effect of fermented rice germ extracts on the inhibition of glucose uptake in the gastrointestinal (GI) tract. Samples were prepared by extracting rice germ fermented with Lactobacillus plantarum with 30% ethanol (RG_30E) or 50% ethanol (RG_50E). Ferulic acid was determined as the active component in the samples. RG_30E significantly inhibited glucose uptake and mRNA expression of GLUT2 and SGLT1 to a larger extent than RG_50E in Caco-2 cells. A single oral administration was performed on C57BL/6 mice to confirm which substrate (glucose, sucrose, or maltose) the sample inhibited absorption of, improving postprandial blood glucose elevation. As a result, RG_30E resulted in significantly lower blood glucose levels and AUC after glucose and sucrose administration. Therefore, fermented rice germ extracted with 30% ethanol regulates glucose uptake through glucose transporters and can be expected to alleviate postprandial hyperglycemia in the GI tract.

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References

  • Adisakwattana S, Chantarasinlapin P, Thammarat H, Yibchok-Anun S. A series of cinnamic acid derivatives and their inhibitory activity on intestinal alpha-glucosidase. Journal of Enzyme Inhibition and Medicinal Chemistry 24: 1194-1200 (2009)

    Article  CAS  Google Scholar 

  • Alzaid F, Cheung HM, Preedy VR, Sharp PA. Regulation of glucose transporter expression in human intestinal Caco-2 cells following exposure to an anthocyanin-rich berry extract. PLoS One1 8: e78932 (2013)

    Article  Google Scholar 

  • Bischoff H. The mechanism of alpha-glucosidase inhibition in the management of diabetes. Clinical and investigative medicine. Medecine clinique et experimentale 18: 303-311 (1995)

    CAS  Google Scholar 

  • Dyer J, Wood I, Palejwala A, Ellis A, Shirazi-Beechey S. Expression of monosaccharide transporters in intestine of diabetic humans. American Journal of Physiology-Gastrointestinal and Liver Physiology (2002)

  • Goto T, Horita M, Nagai H, Nagatomo A, Nishida N, Matsuura Y, Nagaoka S. Tiliroside, a glycosidic flavonoid, inhibits carbohydrate digestion and glucose absorption in the gastrointestinal tract. Molecular Nutrition & Food Research 56: 435-445 (2012)

    Article  CAS  Google Scholar 

  • Huang SH, Ng LT. Quantification of tocopherols, tocotrienols, and gamma-oryzanol contents and their distribution in some commercial rice varieties in Taiwan. Journal of Agricultural and Food Chemistry 59: 11150-11159 (2011)

    Article  CAS  Google Scholar 

  • Hurtado C, Waasdorp C. Carbohydrate digestion and absorption (Naspghan Physiology Series) (2018)

  • Hyun YJ, Kim JG, Jung SK, Kim JY. Fermented rice germ extract ameliorates abnormal glucose metabolism via antioxidant activity in type 2 diabetes mellitus mice. Applied Sciences 11: 3091 (2021)

    Article  CAS  Google Scholar 

  • Johnston K, Sharp P, Clifford M, Morgan L. Dietary polyphenols decrease glucose uptake by human intestinal Caco-2 cells. FEBS Letters 579: 1653-1657 (2005)

    Article  CAS  Google Scholar 

  • Jung EH, Ran Kim S, Hwang IK, Youl Ha T. Hypoglycemic effects of a phenolic acid fraction of rice bran and ferulic acid in C57BL/KsJ-db/db mice. Journal of Agricultural and Food Chemistry 55: 9800-9804 (2007)

    Article  CAS  Google Scholar 

  • Kellett GL, Brot-Laroche E, Mace OJ, Leturque A. Sugar absorption in the intestine: the role of GLUT2. Annual Review of Nutrition 28: 35-54 (2008)

    Article  CAS  Google Scholar 

  • Kelley DE, Bidot P, Freedman Z, Haag B, Podlecki D, Rendell M, Schimel D, Weiss S, Taylor T, Krol A. Efficacy and safety of acarbose in insulin-treated patients with type 2 diabetes. Diabetes Care 21: 2056-2061 (1998)

    Article  CAS  Google Scholar 

  • Kim HK, Baek S-S, Cho H-Y. Inhibitory effect of pomegranate on intestinal sodium dependent glucose uptake. The American Journal of Chinese Medicine 39: 1015-1027 (2011)

    Article  Google Scholar 

  • Kokumai T, Ito J, Kobayashi E, Shimizu N, Hashimoto H, Eitsuka T, Miyazawa T, Nakagawa K. Comparison of blood profiles of γ-oryzanol and ferulic acid in rats after oral intake of γ-oryzanol. Nutrients 11: 1174 (2019)

    Article  CAS  Google Scholar 

  • Laoufi H, Benariba N, Adjdir S, Djaziri R. In vitro α-amylase and α-glucosidase inhibitory activity of Ononis angustissima extracts. Journal of Applied Pharmaceutical Science 7: 191-198 (2017)

    CAS  Google Scholar 

  • Li S, Liu J, Li Z, Wang L, Gao W, Zhang Z, Guo C. Sodium-dependent glucose transporter 1 and glucose transporter 2 mediate intestinal transport of quercetrin in Caco-2 cells. Food & Nutrition Research 64 (2020)

  • Manzano S, Williamson G. Polyphenols and phenolic acids from strawberry and apple decrease glucose uptake and transport by human intestinal Caco-2 cells. Molecular Nutrition & Food Research 54: 1773-1780 (2010)

    Article  CAS  Google Scholar 

  • McGovern PE, Zhang J, Tang J, Zhang Z, Hall GR, Moreau RA, Nuñez A, Butrym ED, Richards MP, Wang C-s. Fermented beverages of pre-and proto-historic China. Proceedings of the National Academy of Sciences 101: 17593-17598 (2004)

  • Moongngarm A, Daomukda N, Khumpika S. Chemical compositions, phytochemicals, and antioxidant capacity of rice bran, rice bran layer, and rice germ. Apcbee Procedia 2: 73-79 (2012)

    Article  CAS  Google Scholar 

  • Narasimhan A, Chinnaiyan M, Karundevi B. Ferulic acid exerts its antidiabetic effect by modulating insulin-signalling molecules in the liver of high-fat diet and fructose-induced type-2 diabetic adult male rat. Applied Physiology, Nutrition, and Metabolism. 40: 769-781 (2015a)

  • Narasimhan A, Chinnaiyan M, Karundevi B. Ferulic acid regulates hepatic GLUT2 gene expression in high fat and fructose-induced type-2 diabetic adult male rat. European Journal of Pharmacology 761: 391-397 (2015b)

    Article  CAS  Google Scholar 

  • Nisa K, Rosyida V, Nurhayati S, Indrianingsih A, Darsih C, Apriyana W. “IOP Conference Series: Earth and Environmental Science,“ IOP Publishing, pp. 012020 (2019)

  • Panfili G, Fratianni A, Irano M. Normal phase high-performance liquid chromatography method for the determination of tocopherols and tocotrienols in cereals. Journal of Agricultural and Food Chemistry 51: 3940-3944 (2003)

    Article  CAS  Google Scholar 

  • Perez-Ternero C, Alvarez de Sotomayor M, Herrera MD. Contribution of ferulic acid, γ-oryzanol and tocotrienols to the cardiometabolic protective effects of rice bran. Journal of Functional Foods 32: 58-71 (2017)

    Article  CAS  Google Scholar 

  • Purves RD. Optimum numerical integration methods for estimation of area-under-the-curve (AUC) and area-under-the-moment-curve (AUMC). Journal of Pharmacokinetics and Biopharmaceutics 20: 211-226 (1992)

    Article  CAS  Google Scholar 

  • Schalkwijk CG, Stehouwer CD. Vascular complications in diabetes mellitus: the role of endothelial dysfunction. Clinical Science 109: 143-159 (2005)

    Article  CAS  Google Scholar 

  • Schmidt CG, Gonçalves LM, Prietto L, Hackbart HS, Furlong EB. Antioxidant activity and enzyme inhibition of phenolic acids from fermented rice bran with fungus Rizhopus oryzae. Food Chemistry 146: 371-377 (2014)

    Article  CAS  Google Scholar 

  • Shamloo M, Jones PJH, Eck PK. Inhibition of intestinal cellular glucose uptake by phenolics extracted from whole wheat grown at different locations. Journal of Nutrition and Metabolism 5421714 (2018)

  • Shimizu M, Kobayashi Y, Suzuki M, Satsu H, Miyamoto Y. Regulation of intestinal glucose transport by tea catechins. BioFactors 13: 61-65 (2000)

    Article  CAS  Google Scholar 

  • Shin HY, Kim SM, Lee JH, Lim ST. Solid-state fermentation of black rice bran with Aspergillus awamori and Aspergillus oryzae: effects on phenolic acid composition and antioxidant activity of bran extracts. Food Chemistry 272: 235-241 (2019)

    Article  CAS  Google Scholar 

  • Sirilun S, Chaiyasut C, Pengkumsri N, Pelyuntha W, Peerajan S, Sivamaruthi BS. Production of ferulic acid from oryzanol degradation during the fermentation of black rice bran by ferulic acid esterase producing Aspergillus oryzae HP (2015)

  • Solares-Pascasio JI, Ceballos G, Calzada F, Barbosa E, Velazquez C. Antihyperglycemic and lipid profile effects of Salvia amarissima Ortega on streptozocin-induced type 2 diabetic mice. Molecules 26: 947 (2021)

    Article  CAS  Google Scholar 

  • Song H-N, Lee YR. Biological activities and quality characteristics of rice germ after microbial fermentation. The Korean Journal of Food And Nutrition 30: 59-66 (2017)

    Article  Google Scholar 

  • Srinivasan M, Sudheer AR, Menon VP. Ferulic acid: therapeutic potential through its antioxidant property. Journal of Clinical Biochemistry and Nutrition 40: 92-100 (2007)

    Article  CAS  Google Scholar 

  • Tanaka M, Yoshino Y, Takeda S, Toda K, Shimoda H, Tsuruma K, Shimazawa M, Hara H. Fermented rice germ extract alleviates morphological and functional damage to murine gastrocnemius muscle by inactivation of AMP-activated protein kinase. Journal of Medicinal Food 20: 969-980 (2017)

    Article  CAS  Google Scholar 

  • Vinayagam R, Jayachandran M, Xu B. Antidiabetic effects of simple phenolic acids: a comprehensive review. Phytotherapy Research 30: 184-199 (2016)

    Article  CAS  Google Scholar 

  • Wang O, Liu J, Cheng Q, Guo X, Wang Y, Zhao L, Zhou F, Ji B. Effects of ferulic acid and gamma-oryzanol on high-fat and high-fructose diet-induced metabolic syndrome in rats. PLoS One1 10: e0118135 (2015)

    Article  Google Scholar 

  • Zheng Y, Scow JS, Duenes JA, Sarr MG. Mechanisms of glucose uptake in intestinal cell lines: role of GLUT2. Surgery 151: 13-25 (2012)

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Agro and Livestock Products Safety·Flow Management Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (119072-1). We appreciate the Cheongwon Saengmyeong Agricultural Cooperative Federation Rice association corporation for supporting the fermented rice germ extract samples.

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Authors and Affiliations

  1. Department of Food Science and Technology, Seoul National University of Science and Technology, 232, Gongneung-ro, Nowon-gu, Seoul, 01811, Republic of Korea

    Ye Ji Hyun, Soo-yeon Park & Ji Yeon Kim

  2. Department of Nano Bio Engineering, Seoul National University of Science and Technology, 232, Gongneung-ro, Nowon-gu, Seoul, 01811, Republic of Korea

    Ji Yeon Kim

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  1. Ye Ji Hyun
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Correspondence to Ji Yeon Kim.

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Hyun, Y.J., Park, Sy. & Kim, J.Y. The effect of fermented rice germ extracts on the inhibition of glucose uptake in the gastrointestinal tract in vitro and in vivo. Food Sci Biotechnol 32, 371–379 (2023). https://doi.org/10.1007/s10068-022-01198-6

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  • DOI: https://doi.org/10.1007/s10068-022-01198-6

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