Skip to main content
Log in

Physicochemical properties and antioxidant activity of colored peppers (Capsicum annuum L.)

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Peppers are spices consumed all around the world. This study evaluated the physicochemical properties and antioxidant activities of red, orange, yellow, green, and purple peppers. Total capsaicinoids showed the highest concentration in the green pepper (2416.50 µg/g). Vitamin C showed similar concentrations in all peppers (28.90–30.95 mg/g), except for the purple pepper (25.59 mg/g). Chlorophyll was abundant in the green and the purple peppers (280.36 and 102.13 mg/100 g). Total carotenoid was abundant in the red and the orange peppers (237.04 and 276.94 mg/100 g). Total anthocyanin was detected only in the purple pepper (67.13 mg/100 g). Total flavonoid showed a high concentration in the green and the purple peppers (24.27 and 22.27 CAE mg/g). The yellow pepper showed the highest antioxidant activity according to total polyphenol, DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power assays. Therefore, peppers showed potential for the development of functional food materials.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Borovsky Y, Paran I. Chlorophyll breakdown during pepper fruit ripening in the chlorophyll retainer mutation is impaired at the homolog of the senescence-inducible stay-green gene. Theoretical and Applied Genetics. 117: 235-240 (2008)

    Article  CAS  Google Scholar 

  • Carvalho Lemos V, Reimer JJ, Wormit A. Color for life: Biosynthesis and distribution of phenolic compounds in pepper (Capsicum annuum). Agriculture. 9: 81 (2019)

    Article  Google Scholar 

  • Chang CC, Yang MH, Wen HM, Chen JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 10: 178-182 (2002)

    CAS  Google Scholar 

  • Chappelle EW, Kim MS, McMurtrey JE. Ratio analysis of reflectance spectra (RARS): An algorithm for the remote estimation of the concentrations of chlorophyll a, chlorophyll b, and carotenoids in soybean leaves. Remote Sensing of Environment. 39: 239-247 (1992)

    Article  Google Scholar 

  • Deepa N, Kaur C, Singh B, Kapoor HC. Antioxidant activity in some red sweet pepper cultivars. Journal of Food Composition and Analysis. 19: 572-578 (2006)

    Article  CAS  Google Scholar 

  • Di Gioia F, Tzortzakis N, Rouphael Y, Kyriacou MC, Sampaio SL, CFR Ferreira I, Petropoulos SA. Grown to be blue-Antioxidant properties and health effects of colored vegetables. Part II: leafy, fruit, and other vegetables. Antioxidants 9: 97 (2020)

  • Giuffrida D, Dugo P, Torre G, Bignardi C, Cavazza A, Corradini C, Dugo G. Characterization of 12 Capsicum varieties by evaluation of their carotenoid profile and pungency determination. Food Chemistry. 140: 794-802 (2013)

    Article  CAS  Google Scholar 

  • Guil-Guerrero JL, Martinez-Guirado C, Rebolloso-Fuentes MM, Carrique-Perez A. Nutrient composition and antioxidant activity of 10 pepper (Capsicum annuun) varieties. European Food Research and Technology. 224: 1-9 (2006)

    Article  CAS  Google Scholar 

  • Hosseinian FS, Li W, Beta T. Measurement of anthocyanins and other phytochemicals in purple wheat. Food Chemistry. 109: 916-924 (2008)

    Article  CAS  Google Scholar 

  • Howard LR, Wildman REC. In Handbook of Nutraceuticals and Functional Foods. 2nd ed. CRC Press, Boca Raton, FL, USA. pp. 165-199 (2007)

    Google Scholar 

  • Howard LR, Talcott ST, Brenes CH, Villalon B. Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum Species) as influenced by maturity. Journal of Agricultural and Food Chemistry. 48: 1713-1720 (2000)

    Article  CAS  Google Scholar 

  • Huang Y, So YJ, Hwang JR, Yoo KM, Lee KW, Lee YJ, Hwang IK. Comparative studies on phytochemicals and bioactive activities in 24 new varieties of red pepper. Korean Journal of Food Science and Technology. 46: 395-403 (2014)

    Article  Google Scholar 

  • Hwang JR, Hwang IK, Kim SN. Quantitative analysis of various carotenoids from different colored paprika using UPLC. Korean Journal of Food Science and Technology. 47: 1-5 (2015)

    Article  Google Scholar 

  • Kim S, Park JB, Hwang IK. Quality attributes of various varieties of Korean red pepper powders (Capsicum annuum L.) and color stability during sunlight exposure. Journal of Food Science 67: 2957-2961 (2002)

  • Kim TJ, Choi JH, Kim KW, Ahn SK, Ha SH, Choi YS, Park NI, Kim JK. Metabolite profiling of peppers of various colors reveals relationships between tocopherol, carotenoid, and phytosterol content. Journal of Food Science. 82: 2885-2893 (2007)

    Article  Google Scholar 

  • Lee Y, Howard LR, Villalon B. Flavonoids and antioxidant activity of fresh pepper (Capsicum annuum) cultivars. Journal of Food Science. 60: 473-476 (1995)

    Article  CAS  Google Scholar 

  • Lee MH, Han JS, Kozukue N. Changes of chlorophyll contents in spinach by growth periods and Storage. Korean Society of Food and Cookery Science. 21: 339-345 (2005)

    Google Scholar 

  • Lee IS, Lee HJ, Cho EY, Kwon SB, Lee JS, Jeong HS, Hwang Y, Cho MC, Kim HR, Yoo SM, Kim HY. Spicy hot flavor grading in hot pepper powder for Gochujang in various cultivars using sensory characteristics. The Korean Journal of Community Living Science. 22: 351-364 (2011)

    Article  Google Scholar 

  • Manikharda, Takahashi M, Arakaki M, Yonamine K, Hashimoto F, Takara K, Wada K. Influence of fruit ripening on color, organic acid contents, capsaicinoids, aroma compounds, and antioxidant capacity of Shimatogarashi (Capsicum frutescens). Journal of Oleo Science 67: 113-123 (2018)

  • Materska M, Perucka I. Antioxidant activity of the main phenolic compounds isolated from hot pepper fruit (Capsicum annuum L.). Journal of Agricultural and Food Chemistry. 53: 1750-1756 (2005)

    Article  CAS  Google Scholar 

  • Matsufuji H, Nakamura H, Chino M, Takeda M. Antioxidant activity of capsanthin and the fatty acid esters in paprika (Capsicum annuum). Journal of Agricultural and Food Chemistry. 46: 3468-3472 (1998)

    Article  CAS  Google Scholar 

  • McGuire RG. Reporting of objective color measurements. HortScience. 27: 1254-1255 (1992)

    Article  Google Scholar 

  • Nadeem M, Anjum FM, Khan MR, Saeed M, Riaz A. Antioxidant potential of bell pepper (Capsicum annum L.)-A review. Pakistan Journal of Food Sciences. 21: 45-51 (2011)

    Google Scholar 

  • Otunola GA, Afolayan AJ. Evaluation of the polyphenolic contents and some antioxidant properties of aqueous extracts of garlic, ginger, cayenne pepper and their mixture. Journal of Applied Botany and Food Quality. 86: 66-70 (2013)

    Google Scholar 

  • Park SY, Lee SY, Yang JW, Lee JS, Oh SD, Oh SW, Lee MS, Lim MH, Park SK, Jang JS, Cho HS, Yeo YS. Comparative analysis of phytochemicals and polar metabolites from colored sweet potato (Ipomoea batatas L.) tubers. Food Science and Biotechnology. 25: 283-291 (2016)

    Article  CAS  Google Scholar 

  • Schweiggert U, Kammerer DR, Carle R, Schieber A. Characterization of carotenoids and carotenoid esters in red pepper pods (Capsicum annuum L.) by high-performance liquid. Rapid Communications in Mass Spectrometry. 19: 2617-2628 (2005)

    Article  CAS  Google Scholar 

  • Scoville WL. Note on Capsicum. Journal of the American Pharmaceutical Association. 1: 453-454 (1912)

    CAS  Google Scholar 

  • Simonne AH, Simonne EH, Eitenmiller RR, Mills HA, Green NR. Ascorbic acid and provitamin A contents in unusually colored bell peppers (Capsicum annuum L.). Journal of Food Composition and Analysis. 10: 299-311 (1997)

    Article  CAS  Google Scholar 

  • Škrovánková S, Mlček J, Orsavová J, Juríková T, Dřímalová P. Polyphenols content and antioxidant activity of paprika and pepper spices. Potravinarstvo Slovak Journal of Food Sciences. 11: 52-57 (2017)

    Article  Google Scholar 

  • Srinivasan K. Biological activities of red pepper (Capsicum annuum) and its pungent principle capsaicin: a review. Critical Reviews in Food Science and Nutrition. 56: 1488-1500 (2016)

    Article  CAS  Google Scholar 

  • Tang B, Li L, Hu Z, Chen Y, Tan T, Jia Y, Xie Q, Chen G. Anthocyanin accumulation and transcriptional regulation of anthocyanin biosynthesis in purple pepper. Journal of Agricultural and Food Chemistry. 68: 12152-12163 (2020)

    Article  CAS  Google Scholar 

  • Vera-Guzmán MA, Chávez-Servia LJ, Carrillo-Rodríguez CJ, López GM. Phytochemical evaluation of wild and cultivated pepper (Capsicum annuum L. and C. pubescens Ruiz & Pav.) from Oaxaca, Mexico. Chilean Journal of Agricultural Research. 71: 578-585 (2011)

    Article  Google Scholar 

  • Wang MF, Li JG, Rangarajan MR, Shao Y, Lavoie EJ, Huang TC, Ho CT. Antioxidative phenolic compounds from sage (Salivia officinalis). Journal of Agricultural and Food chemistry. 46: 4860-4873 (1998)

    Article  Google Scholar 

  • Weiss EA. Spice crops. CABI Publishing International, New York, NY, USA. pp. 215 (2002)

    Book  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Myung-Hyun Kim or Young-Sil Han.

Ethics declarations

Conflict of interest

No potential conflict of interest relevant to this article was reported.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Choi, MH., Kim, MH. & Han, YS. Physicochemical properties and antioxidant activity of colored peppers (Capsicum annuum L.). Food Sci Biotechnol 32, 209–219 (2023). https://doi.org/10.1007/s10068-022-01177-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-022-01177-x

Keywords

Navigation