Abstract
Peppers are spices consumed all around the world. This study evaluated the physicochemical properties and antioxidant activities of red, orange, yellow, green, and purple peppers. Total capsaicinoids showed the highest concentration in the green pepper (2416.50 µg/g). Vitamin C showed similar concentrations in all peppers (28.90–30.95 mg/g), except for the purple pepper (25.59 mg/g). Chlorophyll was abundant in the green and the purple peppers (280.36 and 102.13 mg/100 g). Total carotenoid was abundant in the red and the orange peppers (237.04 and 276.94 mg/100 g). Total anthocyanin was detected only in the purple pepper (67.13 mg/100 g). Total flavonoid showed a high concentration in the green and the purple peppers (24.27 and 22.27 CAE mg/g). The yellow pepper showed the highest antioxidant activity according to total polyphenol, DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power assays. Therefore, peppers showed potential for the development of functional food materials.
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Borovsky Y, Paran I. Chlorophyll breakdown during pepper fruit ripening in the chlorophyll retainer mutation is impaired at the homolog of the senescence-inducible stay-green gene. Theoretical and Applied Genetics. 117: 235-240 (2008)
Carvalho Lemos V, Reimer JJ, Wormit A. Color for life: Biosynthesis and distribution of phenolic compounds in pepper (Capsicum annuum). Agriculture. 9: 81 (2019)
Chang CC, Yang MH, Wen HM, Chen JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 10: 178-182 (2002)
Chappelle EW, Kim MS, McMurtrey JE. Ratio analysis of reflectance spectra (RARS): An algorithm for the remote estimation of the concentrations of chlorophyll a, chlorophyll b, and carotenoids in soybean leaves. Remote Sensing of Environment. 39: 239-247 (1992)
Deepa N, Kaur C, Singh B, Kapoor HC. Antioxidant activity in some red sweet pepper cultivars. Journal of Food Composition and Analysis. 19: 572-578 (2006)
Di Gioia F, Tzortzakis N, Rouphael Y, Kyriacou MC, Sampaio SL, CFR Ferreira I, Petropoulos SA. Grown to be blue-Antioxidant properties and health effects of colored vegetables. Part II: leafy, fruit, and other vegetables. Antioxidants 9: 97 (2020)
Giuffrida D, Dugo P, Torre G, Bignardi C, Cavazza A, Corradini C, Dugo G. Characterization of 12 Capsicum varieties by evaluation of their carotenoid profile and pungency determination. Food Chemistry. 140: 794-802 (2013)
Guil-Guerrero JL, Martinez-Guirado C, Rebolloso-Fuentes MM, Carrique-Perez A. Nutrient composition and antioxidant activity of 10 pepper (Capsicum annuun) varieties. European Food Research and Technology. 224: 1-9 (2006)
Hosseinian FS, Li W, Beta T. Measurement of anthocyanins and other phytochemicals in purple wheat. Food Chemistry. 109: 916-924 (2008)
Howard LR, Wildman REC. In Handbook of Nutraceuticals and Functional Foods. 2nd ed. CRC Press, Boca Raton, FL, USA. pp. 165-199 (2007)
Howard LR, Talcott ST, Brenes CH, Villalon B. Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum Species) as influenced by maturity. Journal of Agricultural and Food Chemistry. 48: 1713-1720 (2000)
Huang Y, So YJ, Hwang JR, Yoo KM, Lee KW, Lee YJ, Hwang IK. Comparative studies on phytochemicals and bioactive activities in 24 new varieties of red pepper. Korean Journal of Food Science and Technology. 46: 395-403 (2014)
Hwang JR, Hwang IK, Kim SN. Quantitative analysis of various carotenoids from different colored paprika using UPLC. Korean Journal of Food Science and Technology. 47: 1-5 (2015)
Kim S, Park JB, Hwang IK. Quality attributes of various varieties of Korean red pepper powders (Capsicum annuum L.) and color stability during sunlight exposure. Journal of Food Science 67: 2957-2961 (2002)
Kim TJ, Choi JH, Kim KW, Ahn SK, Ha SH, Choi YS, Park NI, Kim JK. Metabolite profiling of peppers of various colors reveals relationships between tocopherol, carotenoid, and phytosterol content. Journal of Food Science. 82: 2885-2893 (2007)
Lee Y, Howard LR, Villalon B. Flavonoids and antioxidant activity of fresh pepper (Capsicum annuum) cultivars. Journal of Food Science. 60: 473-476 (1995)
Lee MH, Han JS, Kozukue N. Changes of chlorophyll contents in spinach by growth periods and Storage. Korean Society of Food and Cookery Science. 21: 339-345 (2005)
Lee IS, Lee HJ, Cho EY, Kwon SB, Lee JS, Jeong HS, Hwang Y, Cho MC, Kim HR, Yoo SM, Kim HY. Spicy hot flavor grading in hot pepper powder for Gochujang in various cultivars using sensory characteristics. The Korean Journal of Community Living Science. 22: 351-364 (2011)
Manikharda, Takahashi M, Arakaki M, Yonamine K, Hashimoto F, Takara K, Wada K. Influence of fruit ripening on color, organic acid contents, capsaicinoids, aroma compounds, and antioxidant capacity of Shimatogarashi (Capsicum frutescens). Journal of Oleo Science 67: 113-123 (2018)
Materska M, Perucka I. Antioxidant activity of the main phenolic compounds isolated from hot pepper fruit (Capsicum annuum L.). Journal of Agricultural and Food Chemistry. 53: 1750-1756 (2005)
Matsufuji H, Nakamura H, Chino M, Takeda M. Antioxidant activity of capsanthin and the fatty acid esters in paprika (Capsicum annuum). Journal of Agricultural and Food Chemistry. 46: 3468-3472 (1998)
McGuire RG. Reporting of objective color measurements. HortScience. 27: 1254-1255 (1992)
Nadeem M, Anjum FM, Khan MR, Saeed M, Riaz A. Antioxidant potential of bell pepper (Capsicum annum L.)-A review. Pakistan Journal of Food Sciences. 21: 45-51 (2011)
Otunola GA, Afolayan AJ. Evaluation of the polyphenolic contents and some antioxidant properties of aqueous extracts of garlic, ginger, cayenne pepper and their mixture. Journal of Applied Botany and Food Quality. 86: 66-70 (2013)
Park SY, Lee SY, Yang JW, Lee JS, Oh SD, Oh SW, Lee MS, Lim MH, Park SK, Jang JS, Cho HS, Yeo YS. Comparative analysis of phytochemicals and polar metabolites from colored sweet potato (Ipomoea batatas L.) tubers. Food Science and Biotechnology. 25: 283-291 (2016)
Schweiggert U, Kammerer DR, Carle R, Schieber A. Characterization of carotenoids and carotenoid esters in red pepper pods (Capsicum annuum L.) by high-performance liquid. Rapid Communications in Mass Spectrometry. 19: 2617-2628 (2005)
Scoville WL. Note on Capsicum. Journal of the American Pharmaceutical Association. 1: 453-454 (1912)
Simonne AH, Simonne EH, Eitenmiller RR, Mills HA, Green NR. Ascorbic acid and provitamin A contents in unusually colored bell peppers (Capsicum annuum L.). Journal of Food Composition and Analysis. 10: 299-311 (1997)
Škrovánková S, Mlček J, Orsavová J, Juríková T, Dřímalová P. Polyphenols content and antioxidant activity of paprika and pepper spices. Potravinarstvo Slovak Journal of Food Sciences. 11: 52-57 (2017)
Srinivasan K. Biological activities of red pepper (Capsicum annuum) and its pungent principle capsaicin: a review. Critical Reviews in Food Science and Nutrition. 56: 1488-1500 (2016)
Tang B, Li L, Hu Z, Chen Y, Tan T, Jia Y, Xie Q, Chen G. Anthocyanin accumulation and transcriptional regulation of anthocyanin biosynthesis in purple pepper. Journal of Agricultural and Food Chemistry. 68: 12152-12163 (2020)
Vera-Guzmán MA, Chávez-Servia LJ, Carrillo-Rodríguez CJ, López GM. Phytochemical evaluation of wild and cultivated pepper (Capsicum annuum L. and C. pubescens Ruiz & Pav.) from Oaxaca, Mexico. Chilean Journal of Agricultural Research. 71: 578-585 (2011)
Wang MF, Li JG, Rangarajan MR, Shao Y, Lavoie EJ, Huang TC, Ho CT. Antioxidative phenolic compounds from sage (Salivia officinalis). Journal of Agricultural and Food chemistry. 46: 4860-4873 (1998)
Weiss EA. Spice crops. CABI Publishing International, New York, NY, USA. pp. 215 (2002)
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Choi, MH., Kim, MH. & Han, YS. Physicochemical properties and antioxidant activity of colored peppers (Capsicum annuum L.). Food Sci Biotechnol 32, 209–219 (2023). https://doi.org/10.1007/s10068-022-01177-x
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DOI: https://doi.org/10.1007/s10068-022-01177-x