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Impact of different extraction conditions on yield, physicochemical and functional characteristics of gelatin from Labeo rohita swim bladder

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Abstract

Influences of different extraction temperatures (50, 60, and 70 °C) and time (3, 6, and 9 h) on yield, physicochemical, and functional properties of gelatin from swim bladder (SBG) of rohu (Labeo rohita) were investigated. Gelatin extracted at 60 °C (G60-9 h), and 70 °C (G70-9 h) temperature for 9 h showed highest gelatin yield 54.91 g/100 g, protein (92.35 g/100 g), and hydroxyproline (77.50 mg/g) content (P < 0.05) compared to other counterparts. All extracted gelatin showed major protein components as α-, β-, and γ-chain with slight changes in band intensity. FTIR spectra showed significant loss of molecular order of triple-helix as temperature and time increased. Furthermore, G60-9 h exhibited higher emulsion, foaming, and gel strength than G70-9 h, and G50-9 h, respectively (P < 0.05). Therefore, G60-9 h could be appropriate conditions for the extraction of SBG with better yield, physicochemical, and functional properties.

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References

  • Ahmad M, Benjakul S. Characteristics of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as influenced by acid pretreatment and extraction time. Food Hydrocolloids 25: 381-388 (2011)

    Article  CAS  Google Scholar 

  • AOAC. Official methods of analysis (17th ed.) Arlington: Association of Official Analytical Chemists Inc (2000)

    Google Scholar 

  • Arnesen JA, Gildberg A. Extraction and characterisation of gelatine from Atlantic salmon (Salmo salar) skin. Bioresource Technology. 98: 53-57 (2007)

    Article  CAS  Google Scholar 

  • Balti R, Jridi M, Sila A, Souissi N, Nedjar-Arroume N, Guillochon D, Nasri M. Extraction and functional properties of gelatin from the skin of cuttlefish (Sepia officinalis) using smooth hound crude acid protease-aided process. Food Hydrocolloids. 25: 943-950 (2011)

    Article  CAS  Google Scholar 

  • Bandekar J. Amide modes and protein conformation. Biochimica et Biophysica Acta-Protein Structure and Molecular Enzymology. 1120: 123-143 (1992)

    Article  CAS  Google Scholar 

  • Benjakul S, Oungbho K, Visessanguan W, Thiansilakul Y, Roytrakul S. Characteristics of gelatin from the skins of bigeye snapper, Priacanthus tayenus and Priacanthus macracanthus. Food Chemistry. 116: 445-451(2009)

    Article  CAS  Google Scholar 

  • Bergman I, Loxley R. Two improved and simplified methods for the spectrophotometric determination of hydroxyproline. Analytical Chemistry. 35: 1961-1965 (1963)

    Article  CAS  Google Scholar 

  • Dalev PG, Patil RD, Mark JE, Vassileva E, Fakirov S. Biodegradation of chemically modified gelatin films in soil. Journal of Applied Polymer Science. 78: 1341-1347 (2000)

    Article  CAS  Google Scholar 

  • Fernandez-Dıaz MD, Montero P, Gomez-Guillen MC. Gel properties of collagens from skins of cod (Gadus morhua) and hake (Merluccius merluccius) and their modification by the coenhancers magnesium sulphate, glycerol and transglutaminase. Food Chemistry. 74: 161-167 (2001)

    Article  Google Scholar 

  • Gómez-Guillén MC, Giménez B, López-Caballero MA, Montero MP. Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocolloids. 25: 1813-1827 (2011)

    Article  Google Scholar 

  • GME Standard methods for the testing of edible gelatine, Gelatine Monograph. Gelatin Manufacturers of Europe (2005)

  • Hailing PJ, Walstra P. Protein-stabilized foams and emulsions. Critical Review in Food Science and Nutrition. 15: 155-203 (1981)

    Article  Google Scholar 

  • Irwandi J, Faridayanti S, Mohamed ESM, Hamzah MS, Torla HH, Che Man YB. Extraction and characterization of gelatin from different marine fish species in Malaysia. International Food Research Journal. 16: 381–389 (2009)

  • Jackson M, Watson PH, Halliday WC, Mantsch HH. Beware of connective tissue proteins: assignment and implications of collagen absorptions in infrared spectra of human tissues. Biochimica et Biophysica Acta - Molecular Basis of Disease. 1270(1): 1-6 (1995)

    Article  Google Scholar 

  • Jamilah B, Harvinder KG. Properties of gelatins from skins of fish black tilapia (Oreochromis mossambicus) and red tilapia (Oreochromis nilotica). Food Chemistry. 77: 81–84 (2002)

    Article  CAS  Google Scholar 

  • Jellouli K, Balti R, Bougatef A, Hmidet N, Barkia A, Nasri M. Chemical composition and characteristics of skin gelatin from grey triggerfish (Balistes capriscus). LWT - Food Science & Technology. 44: 1965-1970 (2011)

    Article  CAS  Google Scholar 

  • Jones NR. Uses of gelatin in edible products. In A. G. Ward, & A. Courts (Eds.), The Science and Technology of Gelatin (366–392) Academic Press, London (1977)

    Google Scholar 

  • Kaewdang O, Benjakul S, Kaewmanee T, Kishimura H. Characteristics of collagens from the swim bladders of yellowfin tuna (Thunnus albacares). Food Chemistry. 155: 264-270 (2014)

    Article  CAS  Google Scholar 

  • Kaewruang P, Benjakul S, Prodpran T, Nalinanon S. Physicochemical and functional properties of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as affected by extraction conditions. Food Bioscience. 2: 1-9 (2013)

    Article  CAS  Google Scholar 

  • Kanwate BW, Kudre TG. Effect of various acids on physicochemical and functional characteristics of gelatin from swim bladder of rohu (Labeo rohita). Journal of Food Science and Technology. 54: 2540-2550 (2017)

    Article  CAS  Google Scholar 

  • Kanwate BW, Ballari RV, Kudre TG. Influence of spray-drying, freeze-drying and vacuum-drying on physicochemical and functional properties of gelatin from Labeo rohita swim bladder. International Journal of Biological Macromolecule. 121: 135-141(2019)

    Article  CAS  Google Scholar 

  • Kittiphattanabawon P, Benjakul S, Visessanguan W, Kishimura H, Shahidi F. Isolation and characterisation of collagen from the skin of brownbanded bamboo shark (Chiloscyllium punctatum). Food Chemistry. 119: 1519-1526 (2010)

    Article  CAS  Google Scholar 

  • Kittiphattanabawon P, Benjakul S, Sinthusamran S, Kishimura H. Gelatin from clown featherback skin: Extraction conditions. LWT - Food Science and Technology. 66: 186-192 (2016)

    Article  CAS  Google Scholar 

  • Koli JM, Sagar BV, Kamble RS, Sharangdhar ST. Functional properties of gelatin extracted from four different types of fishes: a comparative study. Indian Journal of Fundamental and Applied Life Science. 4: 322-327 (2014)

    Google Scholar 

  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685 (1970)

    Article  CAS  Google Scholar 

  • Liu J, Xu G, Yuan S, Jiang P. The effect of macromolecules on foam stability in sodium dodecyl sulfate/cetylpyridinium bromide mixtures. Journal of Dispersion Science and Technology. 24: 779-787 (2003)

    Article  Google Scholar 

  • Ledward DA. Gelation of gelatin. Functional-Properties of Food Macromolecules. 171–201(1986)

  • Muyonga JH, Cole CG, Duodu KG. Characterisation of acid soluble collagen from skins of young and adult Nile perch (Lates niloticus). Food chemistry. 85: 81-89 (2004)

    Article  CAS  Google Scholar 

  • Nagarajan M, Benjakul S, Prodpran T, Songtipya P, Kishimura H. Characteristics and functional properties of gelatin from splendid squid (Loligo formosana) skin as affected by extraction temperatures. Food Hydrocolloids. 29: 389-397 (2012)

    Article  CAS  Google Scholar 

  • Pearce KN, Kinsella JE. Emulsifying properties of proteins: evaluation of a turbidimetric technique. Journal of Agricultural and Food Chemistry. 26: 716-723 (1978)

    Article  CAS  Google Scholar 

  • Pranoto Y, Marseno DW, Rahmawati H. Characteristics of gelatins extracted from fresh and sun-dried seawater fish skins in Indonesia. International Food Research Journal. 18: 1335-1341 (2011)

    CAS  Google Scholar 

  • Ratnasari, I., Firlianty. Physico-chemical characterization and skin gelatin rheology of four freshwater fish as alternative gelatin source. Bioflux. 9: 1196-1207 (2016)

    Google Scholar 

  • Shahidi F, Han XQ, Synowiecki J. Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food chemistry. 53: 285-293 (1995)

    Article  CAS  Google Scholar 

  • Sinthusamran S, Benjakul S, Kishimura H. Comparative study on molecular characteristics of acid soluble collagens from skin and swim bladder of seabass (Lates calcarifer). Food Chemistry. 138: 2435-2441(2013)

    Article  CAS  Google Scholar 

  • Steel RGD, Torrie JH. Principles and procedures of statistics: A biometrical approach (2nd ed.) New York: McGraw-Hill (1980)

    Google Scholar 

  • Surewicz WK, Mantsch HH. New insight into protein secondary structure from resolution-enhanced infrared spectra. Biochimica et Biophysica Acta-Protein Structure and Molecular Enzymology. 952: 115-130 (1988)

  • Tan CC, Karim AA, Uthumporn U, Ghazali FC. Effect of extraction temperature on the physicochemical properties of gelatine from the skin of black tilapia (Oreochromis mossambicus). Journal of Physical Science. 30: 1-21(2019)

    Article  CAS  Google Scholar 

  • Van der Ven C, Gruppen H, de Bont DB, Voragen AG. Correlations between biochemical characteristics and foam-forming and-stabilizing ability of whey and casein hydrolysates. Journal of Agricultural and Food Chemistry. 50: 2938-2946 (2002)

    Article  Google Scholar 

  • Weber P, Steinhart H, Paschke. A Characterization, antigenicity and detection of fish gelatine and isinglass used as processing aids in wines. Food Additives & Contaminants: Part A. 27: 273-282 (2010)

    Article  CAS  Google Scholar 

  • Yakimets I, Wellner N, Smith AC, Wilson RH, Farhat I, Mitchell J. Mechanical properties with respect to water content of gelatin films in glassy state. Polymer. 46: 12577-12585 (2005)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank the Council of Scientific and Industrial Research (CSIR), New Delhi, India, for the award of the Research Fellowship. The authors would like to express their sincere thanks to the Director, CSIR-Central Food Technological Research Institute, Mysuru, India, for the support, encouragement, and permission to publish this work.

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Authors and Affiliations

  1. Meat and Marine Sciences Department, CSIR-Central Food Technological Research Institute, 570020, Mysuru, Karnataka, India

    Balaji Wamanrao Kanwate & Tanaji G. Kudre

  2. Academy of Scientific and Innovative Research (AcSIR), 201002, Ghaziabad, India

    Balaji Wamanrao Kanwate & Tanaji G. Kudre

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  1. Balaji Wamanrao Kanwate
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  2. Tanaji G. Kudre
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BWK Involved in conducting the experiments, collection and analysis of data and manuscript writing. TGK Involved in conception, design and implementation of the research, analysis of data, manuscript writing and overall supervision of the project. All authors discussed the results and contributed to the final manuscript.

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Correspondence to Tanaji G. Kudre.

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Kanwate, B.W., Kudre, T.G. Impact of different extraction conditions on yield, physicochemical and functional characteristics of gelatin from Labeo rohita swim bladder. Food Sci Biotechnol 31, 1277–1287 (2022). https://doi.org/10.1007/s10068-022-01121-z

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