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Phenolic composition and neuroprotective effects of the ethyl-acetate fraction from Inonotus sanghuang against H2O2-induced apoptotic cell death of primary cortical neuronal cells

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Abstract

The study aimed to characterize phenolic compounds of the Inonotus sanghuang’s ethyl-acetate fraction (EAF) and assess the neuroprotective effect of EAF using the H2O2-treated primary cortical neuronal cells (PCNC) model. Using HPLC-ECD, 5 phenolics were identified and quantified from EAF. H2O2-treated PCNC experiments in vitro showed that pretreatment with EAF increased the GSH-PX and SOD activities and reduced the NO, MDA, and Aβ contents. Furthermore, EAF suppressed the production of IL-1β, IFN-γ, IL-6, and TNF-α in H2O2-treated PCNC. Other mechanisms found that EAF reduced Bax, caspase 9, and caspase 3 expressions at the mRNA and protein levels while increasing Bcl-2 expression at the mRNA and protein levels. These results showed that EAF could serve as potential agents for anti-NDD.

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Acknowledgements

This study was supported by the Science Research Program for Colleges and Universities of Hebei Province (ZD2018067), the Key research and development project from Hebei Province (21327109D), Foundation of Hebei University of Economics and Business (2020ZD10).

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Authors and Affiliations

  1. College of Biology Science and Engineering, Hebei University of Economics and Business, Shijiazhuang, 050061, Hebei, China

    Kun Liu & Cuilian Qi

  2. Department of Mathematics and Statistics, Hebei University of Economics and Business, Shijiazhuang, 050061, Hebei, China

    Kun Liu

  3. Department of Computer Science, Wenzhou-Kean University, Wenzhou, 325000, Zhejiang, China

    Yihang Liu

  4. Department of Rehabilitation, Shenzhen Longhua District Central Hospital, Shenzhen, 518000, Guangdong, China

    Yaping Huai

  5. Chronic Disease Rehabilitation Laboratory of Integrated Traditional Chinese and Western Medicine, Beijing Xiaotangshan Hospital, Beijing, 100081, China

    Huagang Hu & Xuan Xiao

  6. Institute of Infection and Immunity of Huaihe Hospital, Henan University, Kaifeng, 475000, Henan, China

    Junpeng Wang

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  1. Kun Liu
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  2. Cuilian Qi
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Correspondence to Yaping Huai.

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Liu, K., Qi, C., Liu, Y. et al. Phenolic composition and neuroprotective effects of the ethyl-acetate fraction from Inonotus sanghuang against H2O2-induced apoptotic cell death of primary cortical neuronal cells. Food Sci Biotechnol 31, 1213–1223 (2022). https://doi.org/10.1007/s10068-022-01107-x

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  • DOI: https://doi.org/10.1007/s10068-022-01107-x

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