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By-product of Korean liquor fermented by Saccharomyces cerevisiae exhibits skin whitening activity

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Herein, the skin whitening effect of the fermentation residue of Saccharomyces cerevisiae was investigated. The fermentation residue showed radical scavenging activity and attenuated tyrosinase activity. Furthermore, the fermentation residue of S. cerevisiae significantly suppressed melanin generation in B16F10 cells. Interestingly, the sample-containing formulation exhibited increased skin whitening activity compared with that by the control formulation in a clinical study. Notably, the endogenous tyrosinase expression was not altered by the fermentation residue of S. cerevisiae; however, the enzymatic activity of tyrosinase was inhibited. Furthermore, the sample did not change TRP1 and TRP2 expression in B16F10 cells. Thus, the fermentation residue of S. cerevisiae was assumed to directly suppress the tyrosinase enzyme. It was confirmed that the fermentation residue of S. cerevisiae was a competitive inhibitor of tyrosinase. Taken together, the fermentation residue of S. cerevisiae could be a novel skin whitening agent originating from the traditional Korean liquor production process.

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  • Badgett A, Milbrandt A. Food waste disposal and utilization in the United States: A spatial cost benefit analysis. Journal of Cleaner Production. 314: 128057 (2021)

    Article  Google Scholar 

  • Borek C. Dietary antioxidants and human cancer. Integrative Cancer Therapies. 3: 333-41 (2004)

    Article  CAS  Google Scholar 

  • Briganti S, Camera E, Picardo M. Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Research. 16: 101-110 (2003)

    Article  Google Scholar 

  • Chin YW, Lee S, Yu HH, Yang SJ, Kim TW. Combinatorial effects of protective agents on survival rate of the yeast starter, Saccharomyces cerevisiae 88-4, after Freeze-Drying. Microorganisms. 9 (2021)

  • Couteau C, Coiffard L. Overview of skin whitening agents: Drugs and cosmetic products. Cosmetics. 3: 27 (2016)

    Article  Google Scholar 

  • D'Orazio JA, Nobuhisa T, Cui RT, Arya M, Spry M, Wakamatsu K, Igras V, Kunisada T, Granter SR, Nishimura EK, Ito S, Fisher DE. Topical drug rescue strategy and skin protection based on the role of Mc1r in UV-induced tanning. Nature. 443: 340-344 (2006)

    Article  CAS  Google Scholar 

  • del Marmol V, Beermann F. Tyrosinase and related proteins in mammalian pigmentation. FEBS Letters. 381: 165-8 (1996)

    Article  Google Scholar 

  • Dreno B, Araviiskaia E, Kerob D, Andriessen A, Anfilova M, Arenbergerova M, Barrios OFL, Mokos ZB, Haedersdal M, Hofmann MA, Khamaysi Z, Kosmadaki M, Lesiak A, Roo E, Zbranca-Toporas A, Wiseman MC, Zimmo S, Guerin L, Fabbrocini G. Nonprescription acne vulgaris treatments: Their role in our treatment armamentarium-An international panel discussion. Journal of Cosmetic Dermatology. 19: 2201-2211 (2020)

    Article  Google Scholar 

  • Eom SJ, Kim TW, Kim S, Kim JH, Park JT, Lee NH, Choi YS, Kang MC, Song KM. Immune-enhancing effects of polysaccharide extract of by-products of Korean liquor fermented by Saccharomyces cerevisiae. International Journal of Biological Macromolecules. 188: 245-252 (2021)

    Article  CAS  Google Scholar 

  • Geronikaki AA, Gavalas AM. Antioxidants and inflammatory disease: synthetic and natural antioxidants with anti-inflammatory activity. Combinatorical Chemistry and High Throughput Screening. 9: 425-42 (2006)

    Article  CAS  Google Scholar 

  • Gueniche A, Bastien P, Ovigne JM, Kermici M, Courchay G, Chevalier V, Breton L, Castiel-Higounenc I. Bifidobacterium longum lysate, a new ingredient for reactive skin. Experimental Dermatology. 19: E1-E8 (2010)

    Article  Google Scholar 

  • Gupta AK, Gover MD, Nouri K, Taylor S. The treatment of melasma: A review of clinical trials. Journal of the American Academy of Dermatology. 55: 1048-1065 (2006)

    Article  Google Scholar 

  • Han SK, Shin HS. Performance of an innovative two-stage process converting food waste to hydrogen and methane. Journal of the Air & Waste Management Association. 54: 242-249 (2004)

    Article  CAS  Google Scholar 

  • Hirobe T. How are proliferation and differentiation of melanocytes regulated? Pigment Cell Melanoma Research. 24: 462-78 (2011)

    Article  CAS  Google Scholar 

  • Jang M, Lee YC, Hong HD, Rhee Y, Lim TG, Kim KT, Chen F, Kim HJ, Cho CW. Anti-oxidative and anti-inflammatory activities of devil's club (Oplopanax horridus) leaves. Food Science and Biotechnology. 26: 213-220 (2017)

    Article  CAS  Google Scholar 

  • Jin YH, Lee SJ, Chung MH, Park JH, Park YI, Cho TH, Lee SK. Aloesin and arbutin inhibit tyrosinase activity in a synergistic manner via a different action mechanism. Archives of Pharmacal Research. 22: 232-6 (1999)

    Article  CAS  Google Scholar 

  • Joshi PG, Nair N, Begum G, Joshi NB, Sinkar VP, Vora S. Melanocyte-keratinocyte interaction induces calcium signalling and melanin transfer to keratinocytes. Pigment Cell Research. 20: 380-384 (2007)

    CAS  PubMed  Google Scholar 

  • Krochmal-Marczak B, Zagorska-Dziok M, Michalak M, Kieltyka-Dadasiewicz A. Comparative assessment of phenolic content, cellular antioxidant, antityrosinase and protective activities on skin cells of extracts from three sweet potato (Ipomoea batatas (L.) Lam.) cultivars. Journal of King Saud University Science. 33 (2021)

  • L'Oréal. The world of beauty in 2020 (2015)

  • Mateus MM, Ventura P, Rego A, Mota C, Castanheira I, Bordado JM, dos Santos RG. Acid liquefaction of potato (Solanum tuberosum) and sweet potato (Ipomoea batatas) cultivars peels pre-screening of antioxidant activity/total phenolic and sugar contents. Bioresources. 12: 1463-1478 (2017)

    Article  CAS  Google Scholar 

  • Miyamoto K, Dissanayake B, Omotezako T, Takemura M, Tsuji G, Furue M. Daily fluctuation of facial pore area, roughness and redness among young Japanese women; Beneficial effects of galactomyces ferment filtrate containing antioxidative skin care formula. Journal of Clinical Medicine. 10(11): 2502 (2021)

  • Morales-Polo C, Cledera-Castro MD, Moratilla Soria BY. Reviewing the anaerobic digestion of food waste: From waste generation and anaerobic process to its perspectives. Applied Sciences-Basel. 8(10): 1084 (2018)

  • Niwano T, Terazawa S, Sato Y, Kato T, Nakajima H, Imokawa G. Glucosamine abrogates the stem cell factor plus endothelin-1-induced stimulation of melanogenesis via a deficiency in MITF expression due to the proteolytic degradation of CREB in human melanocytes. Archives of Dermatological Research. 310: 625-637 (2018)

    Article  CAS  Google Scholar 

  • Ohgidani M, Komizu Y, Goto K, Ueoka R. Antimelanogenic and antioxidative effects of residual powders from Shochu distillation remnants. Food Chemistry. 132: 2140-2143 (2012)

    Article  CAS  Google Scholar 

  • Park SH, Lee HK, Choi HR, Republic of Korea, WO2011126163A1 (2011.10.13).

  • Prochazkova D, Bousova I, Wilhelmova N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 82: 513-23 (2011)

    Article  CAS  Google Scholar 

  • RiceEvans CA, Miller J, Paganga G. Antioxidant properties of phenolic compounds. Trends in Plant Science. 2: 152-159 (1997)

    Article  Google Scholar 

  • Roulin A, Almasi B, Rossi-Pedruzzi A, Ducrest AL, Wakamatsu K, Miksik I, Blount JD, Jenni-Eiermann S, Jenni L. Corticosterone mediates the condition-dependent component of melanin-based coloration. Animal Behaviour. 75: 1351-1358 (2008)

    Article  Google Scholar 

  • Russ W, Meyer-Pittroff R. Utilizing waste products from the food production and processing industries. Critical Reviews in Food Science and Nutrition. 44: 57-62 (2004)

    Article  Google Scholar 

  • Sato K, Morita M, Ichikawa C, Takahashi H, Toriyama M. Depigmenting mechanisms of all-trans retinoic acid and retinol on B16 melanoma cells. Bioscience, Biotechnology and Biochemistry. 72: 2589-97 (2008)

    Article  CAS  Google Scholar 

  • Shen YX, Sun HY, Zeng HY, Prinyawiwatukul W, Xu WQ, Xu ZM. Increases in phenolic, fatty acid, and phytosterol contents and anticancer activities of sweet potato after fermentation by Lactobacillus acidophilus. Journal of Agricultural and Food Chemistry. 66: 2735-2741 (2018)

    Article  CAS  Google Scholar 

  • Singleton VL. Citation classic - colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Current Contents/Agriculture Biology & Environmental Sciences. 18–18 (1985)

  • Song YR, Lim WC, Han A, Lee MH, Shin EJ, Lee KM, Nam TG, Lim TG. Rose petal extract (Rosa gallica) exerts skin whitening and anti-skin wrinkle effects. Journal of Medicinal Food. 23: 870-878 (2020)

    Article  CAS  Google Scholar 

  • Takizawa T, Imai T, Onose J, Ueda M, Tamura T, Mitsumori K, Izumi K, Hirose M. Enhancement of hepatocarcinogenesis by kojic acid in rat two-stage models after initiation with N-bis(2-hydroxypropyl)nitrosamine or N-diethylnitrosamine. Toxicology Science. 81: 43-9 (2004)

    Article  CAS  Google Scholar 

  • Tseng TS, Tsai KC, Chen WC, Wang YT, Lee YC, Lu CK, Don MJ, Chang CY, Lee CH, Lin HH, Hsu HJ, Hsiao NW. Discovery of potent cysteine-containing dipeptide inhibitors against tyrosinase: A comprehensive investigation of 20 x 20 dipeptides in inhibiting dopachrome formation. Journal of Agricultural and Food Chemistry. 63: 6181-6188 (2015)

    Article  CAS  Google Scholar 

  • Tsukamoto K, Jackson IJ, Urabe K, Montague PM, Hearing VJ. A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase. The EMBO Journal. 11: 519-526 (1992)

    Article  CAS  Google Scholar 

  • Vinther J. A guide to the field of palaeo colour: Melanin and other pigments can fossilise: Reconstructing colour patterns from ancient organisms can give new insights to ecology and behaviour. Bioessays. 37: 643-656 (2015)

    Article  Google Scholar 

  • Yang SS, Ling MY. Tetracycline production with sweet-potato residue by solid-state fermentation. Biotechnology and Bioengineering. 33: 1021-1028 (1989)

    Article  CAS  Google Scholar 

  • Zhang LA, Zhao H, Gan MZ, Jin YL, Gao XF, Chen QA, Guan JF, Wang ZY. Application of simultaneous saccharification and fermentation (SSF) from viscosity reducing of raw sweet potato for bioethanol production at laboratory, pilot and industrial scales. Bioresource Technology. 102: 4573-4579 (2011)

    Article  CAS  Google Scholar 

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This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (Nos. 2020R1C1C1004670 and 2018M3C1B505214813).

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Jo, DU., Chin, YW., Kim, Y. et al. By-product of Korean liquor fermented by Saccharomyces cerevisiae exhibits skin whitening activity. Food Sci Biotechnol 31, 587–596 (2022).

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