Skip to main content
SpringerLink
Log in

An improved extraction and purification method for obtaining high-quality chitin and chitosan from blue swimmer (Portunus pelagicus) crab shell waste

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Portunus pelagicus shell waste is highly accumulated in seafood processing factories and has low commercial applications. The objective of this study was to modify and develop a scale-up, simple, and high-yielding chemical method for extraction and purification of chitin and chitosan from P. pelagicus shell waste. The developed method included a new “pretreatment” process using acetic acid followed by chemical treatments at each purification step. The final product was characterized by XRD and FTIR spectroscopy. Control chitin and chitosan were produced using a pre-described method for comparison. Yields of crude chitin, chitosan, and purified chitosan were 32.52 ± 0.68%, 26.28 ± 0.47%, and 21.78 ± 0.34% respectively whereas in the control chitin and chitosan the yields were 20.34 ± 0.72% and 13.79 ± 0.93% respectively (p < 0.05). Better physicochemical and functional properties were recorded in the developed method (p < 0.05). Hence the developed methodology can be scaled up and used in industrial applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • AOAC. Official Method of Analysis of AOAC Intl. 18th ed. Method 930.15. Association of Official Analytical Chemists, Arlington, VA, USA (2005)

  • Brugnerotto J, Lizardi J, Goycoolea FM, Argüelles-Monal W, Desbrières J, Rinaudo M. An infrared investigation in relation with chitin and chitosan characterization. Polymer. 42(8): 3569-3580 (2001)

  • Dash M, Chiellini F, Fernandez EG, Piras AM, Chiellini E. Statistical approach to the spectroscopic determination of the deacetylation degree of chitins and chitosans. Carbohydrate Polymers. 86: 65-71 (2011)

  • Decker EA, Welch B. Role of ferritin as a lipid oxidation catalyst in muscle food. Journal of Agricultural and Food Chemistry. 38: 674-677 (1990)

  • Demir D, Öfkeli F, Ceylan S, Karagülle NB. Extraction and characterization of chitin and chitosan from blue crab and synthesis of chitosan cryogel scaffolds. Journal of the Turkish Chemical Society Section A: Chemistry. 3(3): 131-144 (2016)

  • Domard A. A perspective on 30 years research on chitin and chitosan. Carbohydrate Polymers. 84: 696-703 (2011)

  • Dutta J. Isolation, purification, and nanotechnological applications of chitosan. pp. 1029-1063. In: Polysaccharides Bioactivity and Biotechnology. Ramawat K, Mérillon JM (eds). Springer, Cham, Switzerland (2015)

  • Focher B, Beltrame PL, Naggi A, Torri G. Alkaline N-deacetylation of chitin enhanced by flash treatments. Reaction kinetics and structure modifications. Carbohydrate Polymers. 12(4): 405-418(1990)

  • Goy RC, Morais STB, Assis OBG. Evaluation of the antimicrobial activity of chitosan and its quaternized derivative on E. coli and S. aureus growth. Revista Brasileira de Farmacognosia. 26(1): 122-127 (2016)

  • Hamed I, Özogul F, Regenstein JM. Industrial applications of crustacean by-products (chitin, chitosan, and chitooligosaccharides): A review. Trends in Food Science & Technology. 48: 40-50 (2016)

  • Hassan MA, Omer AM, Abbas E, Baset WMA, Tamer TM. Preparation, physicochemical characterization and antimicrobial activities of novel two phenolic chitosan schiff base derivatives. Scientific Reports. 8(1): 1-14 (2018)

  • Jubert C, Mata JE, Gustafson SB, Dunfield JS. Purification method for biomaterial. U.S. Patent 2009/0137526 A1 (2009)

  • Kaczmarek MB, Struszczyk-Swita K, Li X, Szczęsna-Antczak M, Daroch M. Enzymatic modifications of chitin, chitosan, and chitooligosaccharides. Frontiers in Bioengineering and Biotechnology. 7: 243 (2019)

  • Knidri HE, Dahmani J, Addaou A, Laajeb A, Lahsini A. Rapid and efficient extraction of chitin and chitosan for scale-up production: Effect of process parameters on deacetylation degree and molecular weight. International Journal of Biological Macromolecules. 139: 1092-1102 (2019)

  • Knorr D. Functional Properties of Chitin and Chitosan. Journal of Food Science. 47: 593-595 (1982)

  • Kumari S, Kishor R. Chitin and chitosan: origin, properties, and applications. Vol. I, pp. 1-33. In: Handbook of Chitin and Chitosan. Gopi S, Thomas S, Pius A (eds). Elsevier Inc., Amsterdam, Netherlands (2020)

  • Li Q, Dunn ET, Grandmaison EW, Goosen MFA. Applications and properties of chitosan. Journal of Bioactive and Compatible Polymers. 7: 370-397(1992)

  • Marei NH, Abd El-Samie E, Salah T, Saad GR, Elwahy AH. Isolation and characterization of chitosan from different local insects in Egypt. International journal of biological macromolecules. 82: 871-877 (2016)

  • Mohanasrinivasan V, Mishra M, Paliwal JS, Singh SK, Selvarajan E, Suganthi V, Devi CS. Studies on heavy metal removal efficiency and antibacterial activity of chitosan prepared from shrimp shell waste. 3 Biotech. 4: 167-175 (2013)

  • Moosa AA, Ridha AM, Kadhim NA. Use of biocomposite adsorbents for the removal of methylene blue dye from aqueous solution. American Journal of Materials Science. 6(5): 135-146 (2016)

  • Ngo DH, Kim SK. Antioxidant effects of chitin, chitosan, and their derivatives. Advances in Food and Nutrition Research. 73: 15-31(2014)

  • No HK, Meyers SP, Lee KS. Isolation and characterization of chitin from crawfish shell waste. Journal of Agricultural and Food Chemistry. 37(3): 575–579 (1989)

  • Pambudi GBR, Ulfin I, Harmami H, Suprapto S, Kurniawan F, Ni’mah YL. Synthesis of water-soluble chitosan from crab shells (Scylla serrata) waste. pp. 020086-1–020086-8. In: The 3rd International Seminar on Chemistry, AIP Conference Proceedings 2049. December 18, AIP Publishing LLC (2018)

  • Panith N, Wichaphon J, Lertsiri S, Niamsiri N. Effect of physical and physicochemical characteristics of chitosan on fat-binding capacities under in vitro gastrointestinal conditions. LWT - Food Science and Technology. 71: 25-32 (2016)

  • Parthiban F, Balasundari S, Gopalakannan A, Rathnakumar K, Felix S. Comparison of the quality of chitin and chitosan from shrimp, crab, and squilla waste. Current World Environment. 12: 672-679 (2017)

  • Percot A, Viton C, Domard A. Optimization of chitin extraction from shrimp shells. Biomacromolecules. 4: 12-18 (2003)

  • Poeloengasih CD, Hernawan H, Angwar M. Isolation and characterization of chitin and chitosan prepared under various processing times. Indonesian Journal of Chemistry. 8(2): 189-192 (2008)

  • Puvvada YS, Vankayalapati S, Sukhavasi S. Extraction of chitin from chitosan from exoskeleton of shrimp for application in the pharmaceutical industry. International Current Pharmaceutical Journal. 1(9): 258-263 (2012)

  • Rinaudo M. Chitin and chitosan: properties and applications. Progress in Polymer Science. 31(7): 603-632 (2006)

  • Rout SK. Physicochemical, functional, and spectroscopic analysis of crawfish chitin and chitosan as affected by process modification. PhD thesis. Louisiana State University, Baton Rouge, LA, USA (2001)

  • Sagheer FAA, Al-Sughayer MA, Muslim S, Elsabee MZ. Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf. Carbohydrate. Polymers. 77(2): 410-419 (2009)

  • Sarbon NM, Sandanamsamy S, Kamaruzaman SFS, Ahmad F. Chitosan extracted from mud crab (Scylla olivicea) shells: physicochemical and antioxidant properties. Journal of Food Science and Technology. 52(7): 4266-4275 (2014)

  • Si Trung T, Bao HND. Physicochemical properties and antioxidant activity of chitin and chitosan prepared from pacific white shrimp waste. International Journal of Carbohydrate Chemistry. 2015: Article ID 706259 (2015)

  • Yadav M, Goswami P, Paritosh K, Kumar M, Pareek N, Vivekanand V. Seafood waste: a source for preparation of commercially employable chitin/chitosan materials. Bioresources and Bioprocessing. 6(8): 1-20 (2019)

Download references

Acknowledgements

No funds were received to complete this research.

Author information

Authors and Affiliations

  1. Department of Animal Science, Uva Wellassa University, Badulla, 90000, Sri Lanka

    Wernakulasooriya Manikkage Jayani Chethana Minoli Tissera & Edirisinghe Dewage Nalaka Sandun Abeyrathne

  2. Department of Science and Technology, Uva Wellassa University, Badulla, 90000, Sri Lanka

    Suramya Indunil Rathnayake

  3. Department of Animal Science & Technology, Sunchon National University, Suncheon, 57922, Korea

    Edirisinghe Dewage Nalaka Sandun Abeyrathne & Ki-Chang Nam

Authors
  1. Wernakulasooriya Manikkage Jayani Chethana Minoli Tissera
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Suramya Indunil Rathnayake
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Edirisinghe Dewage Nalaka Sandun Abeyrathne
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ki-Chang Nam
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ki-Chang Nam.

Ethics declarations

Conflict of interest

None of the authors of this study has any financial interest or conflict with industries or any other parties.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 15 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tissera, W.M.J.C.M., Rathnayake, S.I., Abeyrathne, E.D.N.S. et al. An improved extraction and purification method for obtaining high-quality chitin and chitosan from blue swimmer (Portunus pelagicus) crab shell waste. Food Sci Biotechnol 30, 1645–1655 (2021). https://doi.org/10.1007/s10068-021-01002-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-021-01002-x

Keywords

Search

Navigation