Development of enhanced selective media for detection of Vibrio parahaemolyticus in oysters

Abstract

This study was undertaken to develop enhanced selective media for detection of Vibrio parahaemolyticus in oysters. Primarily, tryptic soy agar (TSA) was supplemented with 4.5–5% NaCl, 0.1–0.5% oxgall, and/or 1–2% sodium citrate, and adjusted to pH 8–9. A total of 21 Vibrio spp., 24 indicators, and 26 food-borne isolates were streaked on the modified media, followed by 24 h of incubation at 37 °C. While all the indicators and isolates failed to grow on TSA containing 5% NaCl, 0.5% oxgall, and 2% sodium citrate (TSAOSS1; pH 9), V. parahaemolyticus was culturable on this selective medium. Particularly, the ability of TSAOSS1 to quantify V. parahaemolyticus in oysters was superior to thiosulphate citrate bile salts sucrose (TCBS) agar. V. parahaemolyticus distinctly produced its white-yellowish, round, and edge-pointed colony on TSAOSS1. TSAOSS1 with high selectivity potentials over TCBS may be a promising alternative for detection of V. parahaemolyticus in seafoods or natural reservoirs.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) [grant number: NRF-2016R1A6A3A 11932794 and NRF-2018R1A6A1A03025159] and Chung-Ang University Graduate Research Scholarship in 2020.

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Correspondence to Sun-Young Lee.

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Yoon, JH., Bae, YM., Song, H. et al. Development of enhanced selective media for detection of Vibrio parahaemolyticus in oysters. Food Sci Biotechnol 30, 475–485 (2021). https://doi.org/10.1007/s10068-021-00877-0

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Keywords

  • Detection
  • Food safety
  • Seafood
  • Selective medium
  • Vibrio parahaemolyticus