An optimized procedure for detection of genetically modified DNA in refined vegetable oils

Abstract

In this study, the amplifiable DNA from refined vegetable oils was isolated by using commercial DNA extraction kits based on the CTAB method in combination with nucleic acid enrichment, and then the presence of genetically modified (GM) soybean and maize DNA in the oils was traced by PCR. The results showed that the duration and intensity of heating had no significant effect on the DNA stability and concentration in oils for a short period, suggesting that DNA in oils could be stably reserved for a certain time, thus making it possible to trace down refined vegetable oils reliably and effectively. The results provided a set of primers suitable for systematic GM oil detection. More importantly, this study made an important contribution to the economical and reliable detection of GM vegetable oils regarding food authenticity issues.

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References

  1. Bogani P, Minunni M, Spiriti MM, Zavaglia M, Tombelli S, Buiatti M, Mascini M. Transgenes monitoring in an industrial soybean processing chain by DNA-based conventional approaches and biosensors. Food Chem. 113: 658-664. (2009)

    CAS  Article  Google Scholar 

  2. Costa J, Mafra I, Amaral JS, Oliveira MBPP. Detection of genetically modified soybean DNA in refined vegetable oils. Eur. Food Res. Technol. 230: 915-923. (2010)

    CAS  Article  Google Scholar 

  3. Costa J, Mafra I, Oliveira MBPP. Advances in vegetable oil authentication by DNA-based markers. Trends Food Sci. Technol. 26: 43-55. (2012)

    CAS  Article  Google Scholar 

  4. Doveri S, Lee D. Development of sensitive crop-specific polymerase chain reaction assays using 5S DNA: Applications in food traceability. J. Agr. Food Chem. 55: 4640-4644. (2007)

    CAS  Article  Google Scholar 

  5. Fraiture MA, Herman P, Taverniers I, De Loose M, Deforce D, Roosens NH. Current and new approaches in GMO detection: challenges and solutions. BioMed Res. Int. 2015: 392872. (2015)

    Article  Google Scholar 

  6. Gryson N, Messens K, Dewettinck K. Influence of different oil-refining parameters and sampling size on the detection of genetically modified DNA in soybean oil. J. Am. Oil Chem. Soc. 81: 231-234. (2004)

    CAS  Article  Google Scholar 

  7. Gryson N, Ronsse F, Messens K, De Loose M, Verleyen T, Dewettinck K. Detection of DNA during the refining of soybean oil. J. Am. Oil Chem. Soc. 79: 171-174. (2002)

    CAS  Article  Google Scholar 

  8. Holden MJ, Levine M, Scholdberg T, Haynes RJ, Jenkins GR. The use of 35S and Tnos expression elements in the measurement of genetically engineered plant materials. Anal. Bioanal. Chem. 396: 2175-2187. (2010)

    CAS  Article  Google Scholar 

  9. Holst-Jensen A, Spilsberg B, Arulandhu AJ, Kok E, Shi JX, Zel J. Application of whole genome shotgun sequencing for detection and characterization of genetically modified organisms and derived products. Anal. Bioanal. Chem. 408: 4595-4614. (2016)

    CAS  Article  Google Scholar 

  10. James D, Schmidt AM, Wall E, Green M, Masri S. Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis. J. Agr. Food Chem. 51: 5829-5834. (2003)

    CAS  Article  Google Scholar 

  11. Lau LT, Collins RA, Yiu SH, Xing J, Yu ACH. Detection and characterization of recombinant DNA in the Roundup Ready (R) soybean insert. Food Control. 15: 471-478. (2004)

    CAS  Article  Google Scholar 

  12. Lipp M, Bluth A, Eyquem F, Kruse L, Schimmel H, Van den Eede G, Anklam E. Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs. Eur. Food Res. Technol. 212: 497-504. (2001)

    CAS  Article  Google Scholar 

  13. Mariod A, Matthaus B, Eichner K, Hussein IH. Effects of processing on the quality and stability of three unconventional Sudanese oils. Eur. J. Lipid Sci. Tech. 108: 298-308. (2006)

    CAS  Article  Google Scholar 

  14. Martin CA, Milinsk MC, Visentainer JV, Matsushita M, De-Souza NE. Trans fatty acid-forming processes in foods: a review. An. Acad. Bras. Cienc. 79: 343-350. (2007)

    CAS  Article  Google Scholar 

  15. Mendoza A, Fernandez S, Cruz MA, Rodriguez-Perez MA, Resendez-Perez D, Saldana HAB. Detection of genetically modified maize food products by the polymerase chain reaction. CyTA. 5: 175-181. (2006)

    CAS  Google Scholar 

  16. Nikolić Z, Vasiljević I, Zdjelar G, Đorđević V, Ignjatov M, Jovičić D, Milošević D. Detection of genetically modified soybean in crude soybean oil. Food Chem. 145: 1072-1075. (2014)

    Article  Google Scholar 

  17. Pauli U, Liniger M, Zimmermann A. Detection of DNA in soybean oil. Z. Lebensm Unters F. A. 207: 264-267. (1998)

    CAS  Article  Google Scholar 

  18. Qian C, Wang R, Wu H, Ping J, Wu J. Recent advances in emerging DNA-based methods for genetically modified organisms (GMOs) rapid detection. Trends Anal. Chem. 109: 19-31. (2018)

    CAS  Article  Google Scholar 

  19. Ramos-Gómez S, Busto MD, Perez-Mateos M, Ortega N. Development of a method to recovery and amplification DNA by real-time PCR from commercial vegetable oils. Food Chem. 158: 374-383. (2014)

    Article  Google Scholar 

  20. Ramos-Gómez S, Busto MD, Albillos SM, Ortega N. Novel qPCR systems for olive (Olea europaea L.) authentication in oils and food. Food Chem. 194: 447-454. (2016)

  21. Scollo F, Egea LA, Gentile A, Malfa SL, Dorado G, Hernandez P. Absolute quantification of olive oil DNA by droplet digital-PCR (ddPCR): Comparison of isolation and amplification methodologies. Food Chem. 213: 388-394. (2019)

    Article  Google Scholar 

  22. Studer E, Rhyner C, Luthy J, Hubner P. Quantitative competitive PCR for the detection of genetically modified soybean and maize. Z. Lebensm Unters F. A. 207: 207-213. (1998)

    CAS  Article  Google Scholar 

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Acknowledgements

The research leading to these results has received the financial support from the National Natural Science Foundation of China (No. 31101890).

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Correspondence to Keji Jiang.

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Duan, Y., Pi, Y., Li, C. et al. An optimized procedure for detection of genetically modified DNA in refined vegetable oils. Food Sci Biotechnol 30, 129–135 (2021). https://doi.org/10.1007/s10068-020-00852-1

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Keywords

  • Refined vegetable oil
  • PCR
  • Enrichment treatment
  • Genetically modified oil detection
  • DNA isolation