The asparagus of Triarrhena lutarioriparia (TL) is a popular vegetable with abundant chemical compounds in China. This study aims to optimize the ultrasound-assisted extraction (UAE) method for its content of total flavonoid and antioxidant activities by response surface methodology (RSM). Box-Behnken design was adopted to evaluate the influences of ethanol concentration, extraction time and solvent-to-sample ratio on the extraction yield of total flavonoid and the antioxidant activity. Considering the maximum content of extracted total flavonoids and antioxidant activity, the optimal extraction conditions were acquired with 70% (v/v) ethanol by UAE for 60 min at a solvent-to-sample ratio of 40 mL/g. The proportion of the extraction of total flavonoid was 15.88 mg/g and antioxidant activity reached 79.53%. The RSM would be recommended as an appropriate model for simultaneous optimization of the UAE conditions for the content of total flavonoid and the antioxidant activity of asparagus of TL.
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This study was funded by Construct program of the key discipline of Chinese Pharmacy in Hunan Province (No. 2014001); Hunan Provincial Science and Technology Department Foundation (No. 2017SK2123); Hunan Provincial Natural Science Foundation of China (No. 2018JJ3876); Changsha Science and Technology Department Foundation (No. kq1701070); Cooperation fund project of Hunan University of Chinese Medicine and Yuanjiang municipal government (No. 201606).
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Cao, Q., Yan, J., Sun, Z. et al. Simultaneous optimization of ultrasound-assisted extraction for total flavonoid content and antioxidant activity of the tender stem of Triarrhena lutarioriparia using response surface methodology. Food Sci Biotechnol 30, 37–45 (2021). https://doi.org/10.1007/s10068-020-00851-2
- Asparagus of Triarrhena lutarioriparia
- Ultrasound-assisted extraction
- Total flavonoid
- Antioxidant activity
- Response surface methodology