Simultaneous optimization of ultrasound-assisted extraction for total flavonoid content and antioxidant activity of the tender stem of Triarrhena lutarioriparia using response surface methodology

Abstract

The asparagus of Triarrhena lutarioriparia (TL) is a popular vegetable with abundant chemical compounds in China. This study aims to optimize the ultrasound-assisted extraction (UAE) method for its content of total flavonoid and antioxidant activities by response surface methodology (RSM). Box-Behnken design was adopted to evaluate the influences of ethanol concentration, extraction time and solvent-to-sample ratio on the extraction yield of total flavonoid and the antioxidant activity. Considering the maximum content of extracted total flavonoids and antioxidant activity, the optimal extraction conditions were acquired with 70% (v/v) ethanol by UAE for 60 min at a solvent-to-sample ratio of 40 mL/g. The proportion of the extraction of total flavonoid was 15.88 mg/g and antioxidant activity reached 79.53%. The RSM would be recommended as an appropriate model for simultaneous optimization of the UAE conditions for the content of total flavonoid and the antioxidant activity of asparagus of TL.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3

References

  1. Ahmad A, Alkharfy KM, Wani TA, Raish M. Application of Box-Behnken design for ultrasonic-assisted extractionof polysaccharides from Paeonia emodi. Int. J. Biol. Macromol. 72: 990-997 (2015)

    CAS  Article  Google Scholar 

  2. Babamoradi N, Yousefi S, Ziarati P. Optimization of ultrasound-assisted extraction of functional polysaccharides from common mullein (Verbascum thapsus L) flowers. J. Food Process Eng. 41: e12851 (2018)

    Article  Google Scholar 

  3. Chen Y, Yin LZ, Zhao L, Shu G, Yuan ZX, Fu HL, Lv C, Lin JC. Optimization of the ultrasound-assisted extraction of antioxidant phloridzin from Lithocarpus polystachyus Rehd. using response surface methodology. J. Sep. Sci. 40: 4329-4337 (2017)

    CAS  Article  Google Scholar 

  4. Chen S, Zeng Z, Hu N, Bai B, Wang H, Suo Y. Simultaneous optimization of the ultrasound-assisted extraction for phenolic compounds content and antioxidant activity of Lycium ruthenicum Murr fruit using response surface methodology. Food Chem. 242: 1-8 (2018)

    CAS  Article  Google Scholar 

  5. Dang N, Huang Z, Li H. Advances in biology and applications of fiber plant Triarrhena lutarioriparia. J. Trop. Subtrop. Bot. 20: 418-424 (2012)

    Google Scholar 

  6. Ferreira SL, Bruns RE, Ferreira HS, Matos GD, David JM, Brandaoão GC, da Silva EG, Portugal LA, dos Reis PS, Souza AS, dos Santos WN. Box-Behnken design: an alternative for the optimization of analytical methods. Anal. Chim. Acta. 597: 179-186 (2007)

    CAS  Article  Google Scholar 

  7. Hao JY, Wan Y, Yao XH, Zhao WG, Hu RZ, Chen C, Li L, Zhang DY, Wu GH. Effect of different planting areas on the chemical compositions and hypoglycemic and antioxidant activities of mulberry leaf extracts in Southern China. PloS One 13: e0198072 (2018)

    Article  Google Scholar 

  8. He L, Li A, Liu X, Zhou P. The nuclear type pattern of Chromosome of Triarrhena lutarioriparia L. Liu, sp nov.. J. Hunan Agric. Univ. 22: 461-465 (1996)

    Google Scholar 

  9. He L, Zhou P, Liu X. Studies on the autotetraploid of Triarrhena lutarioriparia L Liu sp nov. Acta Genet. Sin. 24: 544-549 (1997)

    Google Scholar 

  10. Hwang KT, Jung ST, Lee GD, Chinnan MS, Park YS, Park HJ. Controlling molecular weight and degree of deacetylation of chitosan by response surface methodology. J. Agric. Food Chem. 50: 1876-1882 (2002)

    CAS  Article  Google Scholar 

  11. Jiang B, Liu S, Wang L, Shi Y, Tong Q, Yan J. Optimization of enzymatic extraction process of polysaccharides from asparagus based on antioxidant activities. Hunan J. Tradit. Chin. Med. 35: 157-160 (2019)

    Google Scholar 

  12. Joglekar AM, May AT. Product excellence through design of experiments. Cereal Foods World 32: 857-868 (1987)

    Google Scholar 

  13. Kazemi M, Khodaiyan F, Labbafi M, Saeid Hosseini S, Hojjati M. Pistachio green hull pectin: Optimization of microwave-assisted extraction and evaluation of its physicochemical, structural and functional properties. Food Chem. 271: 663-672 (2019)

    CAS  Article  Google Scholar 

  14. Kim JT, Cha DS, Lee GD, Park TW, Kwon DK, Park HJ. Process optimization of sweet potato pulp-based biodegradable plastics using response surface methodology. J. Appl. Polym. Sci. 83: 423-434 (2002)

    CAS  Article  Google Scholar 

  15. Li H, Li Y, Zhang Y, Shi H, Hu W, Zhang Z. Comparison of refluxing, ultrasonic-and microwave-assisted extraction of dihydromyricetin from Ampelopsis grossedentata. J. AOAC Int. 91: 1278-1283 (2008)

    CAS  Article  Google Scholar 

  16. Liang Y, Liu J, Zhong Q, Shen L, Yao J, Huang T, Zhou T. Determination of major aromatic constituents in vanilla using an on-line supercritical fluid extraction coupled with supercritical fluid chromatography. J. Sep. Sci. 41: 1600-1609 (2018)

    CAS  Article  Google Scholar 

  17. Liao Y, Tan Y, Fang Y, Li R. Determination of microelements in Triarrhena lutarioriparia by atomic absorption spectrometry. Centr. South Pharm. 15: 654-657 (2017)

    Google Scholar 

  18. Liao Y, Tan Y, Fang Y, Li R. Effects of different storage methods on total amino acid content in tender stem of Triarrhena lutarioriparia from Yuanjiang. J. Anhui Agric. Sci. 45: 120-121, 128 (2017)

  19. Liu L. Unique paper-making raw materials in China: Triarrhena sacchariflorus. Plants 17: 6-7 (1990)

    CAS  Google Scholar 

  20. Liu Y, Chen F, Guo H. Optimization of bayberry juice spray drying process using response surface methodology. Food Sci. Biotechnol. 26: 1235-1244 (2017)

    CAS  Article  Google Scholar 

  21. Meng Z, Zhao J, Duan H, Guan Y, Zhao L. Green and efficient extraction of four bioactive flavonoids from Pollen Typhae by ultrasound-assisted deep eutectic solvents extraction. J. Pharm. Biomed. Anal.161: 246-253 (2018)

    CAS  Article  Google Scholar 

  22. Nacu-Briciu RD, Cobzac SC, Baciu S. Optimum ultrasound assisted extraction conditions of some flavonoids from green tea leaves. Control quality of green tea product by TLC fingerprinting. Anal. Lett. 44: 2865-2875 (2011)

  23. Nanjing University of Chinese Medicine. Dictionary of Chinese Medicine. 2nd ed. Shanghai Science & Technology Press, China. pp. 1301 (2014)

  24. Pan H, Zhang Q, Cui K, Chen G, Liu X, Wang L. Optimization of extraction of linarin from Flos chrysanthemi indici by response surface methodology and artificial neural network. J. Sep. Sci. 40: 2062-2070 (2017)

    CAS  Article  Google Scholar 

  25. Prakash Maran J, Manikandan S, Vigna Nivetha C, Dinesh R. Ultrasound assisted extraction of bioactive compounds from Nephelium lappaceum L. fruit peel using central composite face centered response surface design. Arab. J. Chem. 10: s1145-s1157 (2017)

  26. Riciputi Y, Diaz-de-Cerio E, Akyol H, Capanoglu E, Cerretani L, Caboni MF, Verardo V. Establishment of ultrasound-assisted extraction of phenolic compounds from industrial potato by-products using response surface methodology. Food Chem. 269: 258-263 (2018)

    CAS  Article  Google Scholar 

  27. Righi Pessoa da Silva H, da Silva C, Bolanho BC. Ultrasonic-assisted extraction of betalains from red beet (Beta vulgaris L.). J. Food Process Eng. 41: e12833. (2018)

  28. Wang J, Geng Y, Li P, Hu F, Li L. Optimization of ultrasound-assisted extraction procedure to determine total isoflavones in Chinese soybean cheese by Box-Behnken design. Food Anal. Method. 6: 221-226 (2013)

    Article  Google Scholar 

  29. Wu C, Wang F, Liu J, Zou Y, Chen X. A comparison of volatile fractions obtained from Lonicera macranthoides via different extraction processes: ultrasound, microwave, Soxhlet extraction, hydrodistillation, and cold maceration. Integr. Med. Res. 4: 171-177 (2015)

    Article  Google Scholar 

  30. Xiao J, Zhao L, Shi X, Nie C, Deng F. Analysis of nutrients and heavy metals in tender stem of Triarrhena lutarioriparia from the Dongting Lake Area. Food Sci. 36: 104-107 (2015)

    CAS  Google Scholar 

  31. Xiao KF, Liao XF, Cui YJ, Ren CS. Study on the new extraction methods of the flavonoids in Lithocarpus polysachyus Rehd. Food Sci. 25: 112-115 (2014)

    Google Scholar 

  32. Zheng LL, Wen G, Yuan MY, Gao F. Ultrasound-assisted extraction of total flavonoids from corn silk and their antioxidant activity. J. Chem. 21: 1-5 (2016)

    Google Scholar 

Download references

Acknowledgements

This study was funded by Construct program of the key discipline of Chinese Pharmacy in Hunan Province (No. 2014001); Hunan Provincial Science and Technology Department Foundation (No. 2017SK2123); Hunan Provincial Natural Science Foundation of China (No. 2018JJ3876); Changsha Science and Technology Department Foundation (No. kq1701070); Cooperation fund project of Hunan University of Chinese Medicine and Yuanjiang municipal government (No. 201606).

Author information

Affiliations

Authors

Corresponding author

Correspondence to Yuanqing Wang.

Ethics declarations

Conflict of interest

We declare that we have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Cao, Q., Yan, J., Sun, Z. et al. Simultaneous optimization of ultrasound-assisted extraction for total flavonoid content and antioxidant activity of the tender stem of Triarrhena lutarioriparia using response surface methodology. Food Sci Biotechnol 30, 37–45 (2021). https://doi.org/10.1007/s10068-020-00851-2

Download citation

Keywords

  • Asparagus of Triarrhena lutarioriparia
  • Ultrasound-assisted extraction
  • Total flavonoid
  • Antioxidant activity
  • Response surface methodology