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Improving the functional properties of fish gelatin by conjugation with the water-soluble fraction of bitter almond gum

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Abstract

Maillard-based conjugation may be a useful way of improving the functional properties of food biopolymers. In this study, covalent attachment of fish gelatin (FG) and the water-soluble fraction of bitter almond gum (SBAG) was performed through dry heating of FG–SBAG mixtures (1:1, 1:2, and 2:1) for 2 days at 60 °C and 80% relative humidity. The formation of the FG–SBAG conjugates was confirmed by FTIR spectroscopy and the degree of glycosylation (DG). The changing color observed in all FG–SBAG conjugates was indicative of Maillard reactions. Heat stability of conjugates increased with increasing SBAG ratio, and denaturation temperatures were consistent with DG. Conjugation improved radical scavenging activity, water holding capacity, emulsifying, and foaming properties of the FG (p < 0.05). Overall, the FG: SBAG (1:2) conjugate was the optimum combination for improving examined functional properties. The results suggest that this conjugate can potentially serve as a new ingredient in food formulations.

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References

  • Abbasi S. Challenges towards characterization and applications of a novel hydrocolloid: Persian gum. Curr. Opin. Colloid Interface Sci. 28: 37-45 (2017)

    Article  CAS  Google Scholar 

  • Abbasi S, Mohammadi S. Stabilization of milk-orange juice mixture using Persian gum: efficiency and mechanism. Food Biosci. 2: 53-60 (2013)

    Article  CAS  Google Scholar 

  • Al-Hakkak J, Al-Hakkak F. Functional egg white-pectin conjugates prepared by controlled Maillard reaction. J. Food Eng. 100: 152-159 (2010)

    Article  CAS  Google Scholar 

  • Amid BT, Mirhosseini H, Poorazarang H, Mortazavi SA. Implications of partial conjugation of whey protein isolate to durian seed gum through Maillard reactions: foaming properties, water holding capacity, and interfacial activity. Molecules 18: 15110-15125 (2013)

    Article  CAS  Google Scholar 

  • Anvari M, Pan CH, Yoon WB, Chung D. Characterization of fish gelatin-gum Arabic complex coacervates as influenced by phase separation temperature. Int. J. Biol. Macromol. 79: 894-902 (2015)

    Article  CAS  Google Scholar 

  • Azarikia F, Abbasi S. Mechanism of soluble complex formation of milk proteins with native gums (tragacanth and Persian gum). Food Hydrocoll. 59: 35-44 (2016)

    Article  CAS  Google Scholar 

  • Bi B, Yang H, Fang Y, Nishinari K, Phillips GO. Characterization and emulsifying properties of β-lactoglobulin-gum Acacia Seyal conjugates prepared via the Maillard reaction. Food Chem. 214: 614-621 (2017)

    Article  CAS  Google Scholar 

  • Bouaziz F, Helbert CB, Romdhane M Ben, Koubaa M, Bhiri F, Kallel F, Chaari F, Driss D, Buon L, Chaabouni SE. Structural data and biological properties of almond gum oligosaccharide: application to beef meat preservation. Int. J. Biol. Macromol. 72: 472-479 (2014)

    Article  Google Scholar 

  • Chahibakhsh N, Hosseini E, Islam MS, Rahbar AR. Bitter almond gum reduces body mass index, serum triglyceride, hyperinsulinemia, and insulin resistance in overweight subjects with hyperlipidemia. J. Funct. Foods. 55: 343-351 (2019)

    Article  CAS  Google Scholar 

  • Damodaran SR. Protein stabilization of emulsions and foams. J. Food Sci. 70: R54-R66 (2005)

    Article  CAS  Google Scholar 

  • Dabestani M, Yeganehzad S. Effect of Persian gum and xanthan gum on foaming properties and stability of pasteurized fresh egg white foam. Food Hydrocoll. 87: 550-560 (2019)

    Article  CAS  Google Scholar 

  • de Oliveira FC, Coimbra JS dos R, de Oliveira EB, Zuñiga ADG, Rojas EEG. Food protein-polysaccharide conjugates obtained via the Maillard reaction: a review. Crit. Rev. Food Sci. Nutr. 56: 1108-1125 (2016)

  • Dufour E. Principles of infrared spectroscopy, pp. 1-27. In: Infrared Spectroscopy for Food Quality Analysis and Control. Sun DW (ed). Academic Press, New York, USA (2009)

  • Fadavi G, Mohammadifar MA, Zargarran A, Mortazavian AM, Komeili R. Composition and physicochemical properties of Zedo gum exudates from Amygdalus scoparia. Carbohydr. Polym. 101: 1074-1080 (2014)

    Article  CAS  Google Scholar 

  • Feng J, Cai H, Wang H, Li C, Liu S. Improved oxidative stability of fish oil emulsion by grafted ovalbumin-catechin conjugates. Food Chem. 241: 60-69 (2018)

    Article  CAS  Google Scholar 

  • Golkar A, Nasirpour A, Keramat J. Improving the emulsifying properties of β-lactoglobulin–wild almond gum (Amygdalus scoparia Spach) exudate complexes by heat. J. Sci. Food Agric. 97: 341-349 (2017)

    Article  CAS  Google Scholar 

  • Hashemi MM, Aminlari M, Forouzan MM, Moghimi E, Tavana M, Shekarforoush S, Mohammadifar MA. Production and application of lysozyme-gum Arabic conjugate in mayonnaise as a natural preservative and emulsifier. Polish J. Food Nutr. Sci. 68: 33-43 (2018)

    Article  CAS  Google Scholar 

  • Hazaveh P, Mohammadi Nafchi A, Abbaspour H. The effects of sugars on moisture sorption isotherm and functional properties of cold water fish gelatin films. Int. J. Biol. Macromol. 79: 370-376 (2015)

    Article  CAS  Google Scholar 

  • Huang T, Tu ZC, Shangguan X, Sha X, Wang H, Zhang L, Bansal N. Fish gelatin modifications: a comprehensive review. Trends Food Sci. Technol. 86: 260-269 (2019)

    Article  CAS  Google Scholar 

  • Jafari SM, Beheshti P, Assadpoor E. Rheological behavior and stability of d-limonene emulsions made by a novel hydrocolloid (Angum gum) compared with Arabic gum. J. Food Eng. 109: 1-8 (2012)

    Article  CAS  Google Scholar 

  • Karim AA, Bhat R. Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins. Food Hydrocoll. 23: 563-576 (2009)

    Article  CAS  Google Scholar 

  • Kchaou H, Benbettaïeb N, Jridi M, Abdelhedi O, Karbowiak T, Brachais CH, Leonard ML, Debeaufort F, Nasri M. Enhancement of structural, functional, and antioxidant properties of fish gelatin films using Maillard reactions. Food Hydrocoll. 83: 326-339 (2018)

    Article  CAS  Google Scholar 

  • Khalesi H, Emadzadeh B, Kadkhodaee R, Fang Y. Whey protein isolate-Persian gum interaction at neutral pH. Food Hydrocoll. 59: 45-49 (2016)

    Article  CAS  Google Scholar 

  • Khemakhem M, Attia H, Ayadi MA. The effect of pH, sucrose, salt, and hydrocolloid gums on the gelling properties and water holding capacity of egg white gel. Food Hydrocoll. 87:11-19 (2019)

    Article  CAS  Google Scholar 

  • Liu F, Ma C, Gao Y, McClements DJ. Food grade covalent complexes and their application as nutraceutical delivery systems: a review. Compr. Rev. Food Sci. Food Saf. 16: 76-95 (2017)

    Article  CAS  Google Scholar 

  • Muhoza B, Xia S, Cai J, Zhang X, Duhoranimana E, Su J. Gelatin and pectin complex coacervates as carriers for cinnamaldehyde: effect of pectin esterification degree on coacervate formation, and enhanced thermal stability. Food Hydrocoll. 87: 712-722 (2019)

    Article  CAS  Google Scholar 

  • O’Regan J, Mulvihill DM. Heat stability and freeze-thaw stability of oil-in-water emulsions stabilized by sodium caseinate-maltodextrin conjugates. Food Chem. 119: 182-190 (2010)

    Article  Google Scholar 

  • Pearce KN, Kinsella JE. Emulsifying properties of proteins: evaluation of a turbidimetric technique. J. Agric. Food Chem. 26: 716-723 (1978)

    Article  CAS  Google Scholar 

  • Pirestani S, Nasirpour A, Keramat J, Desobry S, Jasniewski J. Effect of glycosylation with gum Arabic by Maillard reaction in a liquid system on the emulsifying properties of canola protein isolate. Carbohydr. Polym. 157: 1620-1627 (2017)

    Article  CAS  Google Scholar 

  • Procházková D, Boušová I, Wilhelmová N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia 82: 513-523 (2011)

    Article  Google Scholar 

  • Ramezani R, Esmailpour M, Aminlari M. Effect of conjugation with glucosamine on the functional properties of lysozyme and casein. J. Sci. Food Agric. 88: 2730-2737 (2008)

    Article  CAS  Google Scholar 

  • Sola RJ, Griebenow K. Effects of glycosylation on the stability of protein pharmaceuticals. J. Pharm. Sci. 98: 1223-1245 (2009)

    Article  CAS  Google Scholar 

  • Tan TC, Alkarkhi AFM, Easa AM. Assessment of the ribose-induced Maillard reaction as a means of gelatine powder identification and quality control. Food Chem. 134: 2430-2436 (2012)

    Article  CAS  Google Scholar 

  • Uriarte-Montoya MH, Santacruz-Ortega H, Cinco-Moroyoqui FJ, Rouzaud-Sández O, Plascencia-Jatomea M, Ezquerra-Brauer JM. Giant squid skin gelatin: chemical composition and biophysical characterization. Food Res. Int. 44: 3243-3249 (2011)

    Article  CAS  Google Scholar 

  • Yao XC, Cao Y, Wu SJ. Antioxidant activity and antibacterial activity of peach gum derived oligosaccharides. Int. J. Biol. Macromol. 62: 1-3 (2013)

    Article  Google Scholar 

  • Zhang B, Guo X, Zhu K, Peng W, Zhou H. Improvement of emulsifying properties of oat protein isolate-dextran conjugates by glycation. Carbohydr. Polym. 127: 168-175 (2015)

    Article  CAS  Google Scholar 

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Acknowledgements

The authors wish to acknowledge Dr. Zohreh Khajeh for the critical reading and copy-editing of the manuscript.

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Correspondence to Ebrahim Hosseini.

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Bostar, M., Hosseini, E. Improving the functional properties of fish gelatin by conjugation with the water-soluble fraction of bitter almond gum. Food Sci Biotechnol 30, 55–63 (2021). https://doi.org/10.1007/s10068-020-00847-y

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  • DOI: https://doi.org/10.1007/s10068-020-00847-y

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