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Performance of concanavalin A-immobilized on polyacrylate beads for the detection of human norovirus and hepatitis A virus in fecal specimens

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Abstract

Quantitative reverse transcription PCR (qRT-PCR) is a sensitive method for the detection of foodborne viruses in fecal samples. However, the performance of qRT-PCR depends on the efficiency of virus concentration methods. In this study, the effect of Concanavalin A (Con A)-immobilized on polyacrylate beads (Con A-PAB) on the qRT-PCR performance, in terms of sensitivity and specificity to detect foodborne viruses in human fecal specimens was compared with commercial viral RNA extraction kit (VRNA). The detection of foodborne viruses by qRT-PCR was validated by viral genome sequencing. Both Con A-PAB and VRNA methods were equally sensitive and specific for detecting hepatitis A virus in fecal specimens. Even though both methods showed high specificity (100% vs. 100%) for detecting human norovirus (HuNoV), Con A-PAB method exhibited higher sensitivity (100% vs. 42.9%) and accuracy (100% vs. 73.3%) compared to VRNA method. In conclusion, the application of Con A-PAB would improve the performance of qRT-PCR for the detection of HuNoV in fecal samples.

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Acknowledgements

We thank the study subjects for their time and participation in this study. This research was supported by the research Grant (2017-C37703) received by J. Kwon from the Korea Basic Science Institute (KBSI).

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Authors and Affiliations

  1. Department of Food Science and Technology and Foodborne Virus Research Center, Chonnam National University, Gwangju, 61186, Republic of Korea

    Songhak Kim, Bipin Vaidya, Se-Young Cho & Duwoon Kim

  2. Department of Nutrition and Food Science, Texas A&M University, College Station, TX, 77843-2252, USA

    Susanne U. Mertens-Talcott, Vinicius Paula Venancio & Boon P. Chew

  3. Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon, 34133, Republic of Korea

    Jong-Am Song & Joseph Kwon

Authors
  1. Songhak Kim
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  2. Susanne U. Mertens-Talcott
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  3. Bipin Vaidya
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  4. Vinicius Paula Venancio
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  5. Se-Young Cho
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  9. Duwoon Kim
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Correspondence to Duwoon Kim.

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Supplementary Table S1

Comparison of Con A-immobilized on polyacrylate beads and QIAamp Viral RNA Extraction Mini Kit methods for the detection of foodborne viruses present in fecal samples and validation of test results by viral genomic sequencing (DOCX 16 kb)

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Kim, S., Mertens-Talcott, S.U., Vaidya, B. et al. Performance of concanavalin A-immobilized on polyacrylate beads for the detection of human norovirus and hepatitis A virus in fecal specimens. Food Sci Biotechnol 29, 1727–1733 (2020). https://doi.org/10.1007/s10068-020-00833-4

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  • DOI: https://doi.org/10.1007/s10068-020-00833-4

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