Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes


This study evaluated the anti-adipogenic effects and mechanisms underlying the action of Lactobacillus fermentum MG4231 and MG4244 strains on adipogenesis and lipid accumulation in 3T3-L1 preadipocytes. Treatment with cell-free extracts (CFEs) from the two strains reduced lipid accumulation and intracellular triglyceride production in 3T3-L1 adipocytes by more than 50%. The inhibitory effects of L. fermentum on lipid accumulation were mediated by the downregulation of FAS and aP2 resulting from the inhibition of PPARγ and C/EBPα gene expression. Moreover, AMPK and HSL phosphorylation was upregulated by CFE treatment. These results indicated that the anti-adipogenic and lipolysis activities of L. fermentum strains were caused by increased AMPK and HSL phosphorylation. Both strains displayed high leucine arylamidase and β-galactosidase enzymatic activity, with excellent adhesion to epithelial cells. Therefore, we identified L. fermentum as potential new probiotics for the prevention of obesity.

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Fig. 1
Fig. 2
Fig. 3



Cell-free extract






Dulbecco modified Eagle’s medium


Fetal bovine serum




Tetrazolium bromide salt


Peroxisome proliferator-activated receptor γ


CCAAT/enhancer-binding protein α


Fatty acid synthase


Adipose-specific fatty acid-binding protein


Sterol regulatory element-binding protein 1


Lipoprotein lipase


Acetyl CoA carboxylase


AMP-activated protein kinase


Phospho-AMP-activated protein kinase


Hormone-sensitive lipase


Phospho-hormone- sensitive lipase


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This work was supported by a project for Collabo R&D between Industry, Academy, and Research Institute funded by the Korea Ministry of SMEs and Startups in 2019 (Project No. S2717946).

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Kim, S., Choi, SI., Jang, M. et al. Anti-adipogenic effect of Lactobacillus fermentum MG4231 and MG4244 through AMPK pathway in 3T3-L1 preadipocytes. Food Sci Biotechnol 29, 1541–1551 (2020).

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  • L. fermentum
  • MG4231
  • MG4244
  • Adipogenesis
  • Probiotics