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Characterization of macrophage stimulating compound in glycated whey protein concentrate

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Abstract

Whey, a by-product of cheese making, is a collection of several milk proteins and has functional and nutritional values. Whey protein concentrate (WPC) exhibits various functional effects by glycation. Studies to find sugar-binding sites in a protein having a functional effect are reported. However, it is rarely clear whether it confirms that glycated single protein exhibits the same effect of protein cluster. This study confirmed which protein sites are responsible for the effect of glycated WPC (G-WPC). β-Lactoglobulin (LG) was the major protein of G-WPC and glycated with lactose. The glycated LG increased the nitric oxide and cytokine secretion similar to G-WPC and peptide sequences of active compound was confirmed using the high molecular weight band of G-WPC. The K151 and K157 residues of LG were modified by glycation with sugar in common with G-WPC. These residues of glycated LG potentially contribute to the immune-modulation effect of G-WPC.

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Acknowledgements

This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2017R1A2B4012182), Main Research Program (E0164400-04) of the Korea Food Research Institute (KFRI) funded by the Ministry of Science, and School of Life Science and Biotechnology for BK21 PLUS, Korea University. The authors thank the Institute of Biomedical Science and Food Safety, CJ-Korea University Food Safety Hall (Seoul, Republic of Korea) for providing the equipment and facilities.

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  1. Department of Biotechnology, College of Life Science and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea

    Su-Hyun Chun & Kwang-Won Lee

  2. College of Pharmacy, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06974, Republic of Korea

    Ha Hyung Kim

  3. Division of Functional Food Research, Korea Food Research Institute, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea

    Yoonsook Kim

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  1. Su-Hyun Chun
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Correspondence to Kwang-Won Lee.

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Chun, SH., Kim, H.H., Kim, Y. et al. Characterization of macrophage stimulating compound in glycated whey protein concentrate. Food Sci Biotechnol 29, 1113–1123 (2020). https://doi.org/10.1007/s10068-020-00746-2

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