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Protein purification from Arachis hypogaea in one step: stability studies and anticarcinogenic analysis

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Abstract

The study involved purification of trypsin inhibitor from the seeds of Indian peanuts (Arachis hypogaea), a member of leguminosae family. The inhibitor was purified to homogeneity via three sequential step procedure i.e., salt precipitation to anion-exchange chromatography. The purity and molecular mass was detected using SDS PAGE analysis i.e. ~ 16 kDa. The purified inhibitor termed as Peanut Trypsin Inhibitor (PTI) which inhibits trypsin belonging to serpins family. Anti- neoplastic potential on breast cancer cells (MCF-7) and normal Human Embryonic Kidney cells (HEK) was determined using MTT assay. PTI exhibited IC50 value of ~ 18.412 µg/mL in HEK cells compared to ~ 9.635 µg/mL in MCF-7 cells. The values were quite comparable to curcumin, the standard anticancer drug demonstrating IC50 values of ~ 21.581 µg/mL and ~ 7.135 µg/mL in HEK and MCF-7 respectively. Therefore, we conclude that PTI may be used as supplement along with the conventional drugs for increased efficacy in the treatment of cancer.

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Acknowledgements

The authors are thankful to Central Instrumentation Facility, Department of Biotechnology, Jamia Millia Islamia, New Delhi to allow the access to instruments for the research work.

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Authors and Affiliations

  1. School of Life Sciences, Jaipur National University, Jaipur, Rajasthan, 302017, India

    Afaque Ahmad & Hirday N. Verma

  2. Department of Biotechnology, Jamia Millia Islamia, New Delhi, 110025, India

    Shahbaz Khan & Kapil Dev

  3. Department of Anatomy, All India Institute of Medical Sciences, New Delhi, 110029, India

    Prahalad Bharti & Kamlesh Pandey

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  1. Afaque Ahmad
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  2. Hirday N. Verma
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  3. Prahalad Bharti
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  4. Kamlesh Pandey
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  6. Kapil Dev
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Correspondence to Kapil Dev.

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Ahmad, A., Verma, H.N., Bharti, P. et al. Protein purification from Arachis hypogaea in one step: stability studies and anticarcinogenic analysis. Food Sci Biotechnol 29, 35–43 (2020). https://doi.org/10.1007/s10068-019-00638-0

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  • DOI: https://doi.org/10.1007/s10068-019-00638-0

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