Abstract
There is growing interest in the antioxidants and antibacterial activity from natural substances. The purpose of the research was to gain and distinguish phenolic substances and organic acids in the Camellia oleifera cake, and to study their antioxidant and antibacterial activities. The extraction and purification of them were achieved by solvent extraction and column separation, respectively. The conclusions displayed that purity of the phenolic substances was 94.1 ± 0.5% w/w and that of organic acid was 96.0 ± 0.3% w/w; Fifteen phenolic substances were certificated using HPLC–ESI–MS technology; oxalic, citric, acetic, malic, and succinic acids are discovered to be main organic acids. In addition, the phenolic substances and organic acids both have good antioxidant activity and obvious inhibition against six species of bacteria. These conclusions can be useful in the reuse of the waste of Camellia oleifera oil industry in the future.
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References
Akram K, Kwon JH. Food irradiation for mushrooms: A review. J. Korean. Orean. Soc. Appl. Biol. 53: 257–265 (2010)
Bespyatykh OY, Kokorina AE, Domskii IA. State of antioxidant system of furbearers after injection of succinic acid. Russ. Agric. Sci. 37: 516–519 (2011)
Booth IR. Regulation of cytoplasmic pH in bacteria. Microbiol. Rev. 49: 359–378 (1985)
Chang C, Chen J. Study on the protective mechanism of organic acids in human umbilical vein endothelial cells. Acta Nutrimenta Sinica. 26: 280–283 (2004)
Chen Y, Xie MY, Nie SP, Li C, Wang YX. Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chem. 107: 231–241 (2008)
Davidson PM, Taylor TM. Chemical Preservatives and Natural Antimicrobial Compounds. Food Microbiology Fundamentals & Frontiers (2013)
Difonzo G, Pasqualone A, Silletti R et al. Use of olive leaf extract to reduce lipid oxidation of baked snacks. Food Res. Int. 108: 48–56 (2018)
Hernández-Corroto E, Marina ML, García MC. Multiple protective effect of peptides released from Olea europaea and Prunus persica seeds against oxidative damage and cancer cell proliferation. Food Res. Int. 106: 458–467 (2018)
Hamilton RJ, Kalu C, Prisk E, Padley FB et al. Chemistry of free radicals in lipids. Food Chem. 60: 193–199 (1997)
Helander LM, Alakomi HL, Latva-Kala K. Characterization of the action of selected essential oil components on Gram-negative bacteria. J. Agric. Food Chem. 46: 3590–3595 (1998)
Hochstein P, Atallah AS. The nature of oxidants and antioxidant systems in the inhibition of mutation and cancer. Mut. Res. 202: 363–375 (1988)
Hsiao C, Siebert KJ. Modeling the inewhibitory effects of organic acids on bacteria. Int. J. Food Microbiol. 47: 189–201 (1999)
Hu JL, Nie SP, Xie MY. Antidiabetic mechanism of dietary polysaccharides based on their gastrointestinal functions. J. Agric. Food Chem. 66: 4781–49849 (2018)
Hu JL, Nie SP, Xie MY. High pressure homogenization increases antioxidant capacity and short-chain fatty acid yield of polysaccharide from seeds of Plantago asiatica L. Food Chem. 138: 2338–2345 (2013)
Ibrahim D, Osman H. Antimicrobial activity of Cassia alata from Malaysia. J. Ethnopharmacol. 45: 151–156 (1995)
Kayashima T, Katayama T. Oxalic acid is available as a natural antioxidant in some systems. Biochimica et Biophysica Acta. 1573(1): 1–3 (2002)
Kirakosyan A, Seymour E, Kaufman OB, Warber S, Bolling S, Chang SC. Antioxidant capacity of polyphenolic extracts from leaves of Crataegus laevigata and Crataegus monogyna (Hawthorn) subjected to drought and cold stress. J. Agric. Food Chem. 51: 3973–3976 (2003)
Masuda T, Inaba Y, Maekawa T, Takeda Y, Yamaguchi H, Nakamoto K, Kuninaga H, Nishizato S, Nonaka A. Simple detection method of powerful antiradical compounds in the raw extract of plants and its application for the identification of antiradical plant constituents. J. Agric. Food Chem. 51: 1831–1838 (2003)
Mattera R, Benvenuto M, Giganti MG, Tresoldi I, Pluchinotta FR, Bergante S, Tettamanti G, Masuelli L, Manzari V, Modesti A, Bei R. Effects of polyphenols on oxidative stress-mediated injury in cardiomyocytes. Nutrients. 9: 523 (2017)
Mau JL, Chen CP, Hsieh PC. Antimicrobial effect of extracts from Chinese chive, cinnamon, and corni fructus. J. Agric. Food Chem. 49: 183–188 (2001)
Milardovic S, Ivekovic D, Grabaric BS. A novel amperometric method for antioxidant activity determination using DPPH free radical. Bioelectrochemistry. 68: 175–180 (2006)
Pokorný, J. Natural antioxidants for food use. Trends. Food Sci. Tec. 2: 223–227 (1991)
Poyrazoglu E, Gokmen V, Artik N. Organic acids and phenolic compounds in pomegranates (Punica grganatum L.) grown in Turkey. J. Food Compos. Anal. 15: 567–575 (2002)
Puupponen-Pimiä R, Nohynek L, Meier C, Kaähkoönen M, Heinonen M, Hopia A, Oksman-Caldentey KM. Antimicrobial properties of phenolic compounds from berries. J. Appl. Microbiol. 90: 494–507 (2001)
Ricke SC. Perspectives on the use of organic acids and short chain fatty acids as antimicrobials. Poultry. Sci. 82: 632–639 (2003)
Sentjurc M, Nemec M, Connor HD, Abram V. Antioxidant activity of Sempervivum tectorum and its components. J. Agric. Food Chem. 51: 2766–2771 (2003)
Shen J, Cao C, Su H, Yang X, Wei Z, Du L. Evidence of gastro-intestinal system as an active and toxic target of sasanqua saponins extract. Exp. Toxicol. Pathol. 60: 43–49 (2008)
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16: 144–158 (1965)
Stammati A, Bonsi P, Zucco F, Moezelaar R, Alakomi HL, von Wright A. Toxicity of selected plant volatiles in microbial and mammalian short-term assays. Food Chem. Toxicol. 37: 813–823 (1999)
van den Berg AJ, Halkes SBA, van Ufford HC, Hoekstra MJ, Beukelman CJ. A novel formulation of metal ions and citric acid reduces reactive oxygen species in vitro. J. Wound. Care. 12: 413–418 (2003)
Zhang EX, Yu LJ, Zhou YL, Xiao X. Studies on the peroxidation of polyunsaturated fatty acid from lipoprotein induced by iron and the evaluation of the anti-oxidative activity of some natural products. Acta Biochimica Et Biophysica Sinica. 28: 218–222 (1996)
Zhang GW, Hu MM, He L, Fu P, Wang L, Zhou J. Optimization of microwave-assisted enzymatic extraction of polyphenols from waste peanut shells and evaluation of its antioxidant and antibacterial activities in vitro. Food Bioprod. Process. 91: 158–168 (2013)
Zhang LL, Wang YM, Xu M, Chen JH. Comparisons of antioxidant activity and total phenolics of Camellia oleifera Abel fruit hull from different regions of China. J. Med. Plants. Res. 4: 1420–1426 (2010)
Acknowledgements
This study was supported by National Natural Science Foundation of China (31770861); Young Elite Scientists Sponsorship Program by CA-ST (2018QNRC001); National Key Research and Development Program of China (2017YFC1600405); Jiangxi Provincial Major Program of Research and Development Foundation (Agriculture field, 20165ABC28004).
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Zhang, D., Nie, S., Xie, M. et al. Antioxidant and antibacterial capabilities of phenolic compounds and organic acids from Camellia oleifera cake. Food Sci Biotechnol 29, 17–25 (2020). https://doi.org/10.1007/s10068-019-00637-1
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DOI: https://doi.org/10.1007/s10068-019-00637-1