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Optimization of process parameters for improved production of biomass protein from Aspergillus niger using banana peel as a substrate

Abstract

This study was aimed to optimize the process variables for improved production of biomass protein using Aspergillus niger from banana fruit peel by the use of response surface methodology. A five-level-four factors central composite rotatable design was applied to elucidate the influence of process variables viz. temperature (20–40 °C), pH (4–8), substrate concentration (5–25%), and fermentation period (1–5 days) on biomass and protein content. The second-order polynomial models were established, which effectively explicated the variation in experimental data and significantly epitomized the appreciable correlation between independent variables and responses. After numerical optimization, the predicted optimum conditions (temperature of 31.02 °C, pH of 6.19, substrate concentration of 19.92%, and the fermentation period of 4 days) were obtained with biomass of 24.69 g/L and protein of 61.23%, which were verified through confirmatory experiments.

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Acknowledgements

We are very grateful to the authority of the Ministry of Science and Technology, Bangladesh for financial support through the “National Science and Technology (NST) Research Fellowship” program (NST 2016-2017(GO-12/603)).

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Correspondence to Shakti Chandra Mondal.

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Kamal, M.M., Ali, M.R., Shishir, M.R.I. et al. Optimization of process parameters for improved production of biomass protein from Aspergillus niger using banana peel as a substrate. Food Sci Biotechnol 28, 1693–1702 (2019). https://doi.org/10.1007/s10068-019-00636-2

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  • DOI: https://doi.org/10.1007/s10068-019-00636-2

Keywords

  • Biomass
  • Protein
  • Aspergillus niger
  • Banana peel
  • Optimization
  • Response surface methodology