Abstract
Quality of beef and detect changes in freshness during storage was evaluated using freshness indicator. The freshness indicator can indicate spoilage or freshness of the packaged product through color changes directly detected by the naked eye. We also monitored changes in pH, 2-thiobarbituric acid reactive substances, volatile basic nitrogen, and total bacterial count. A change in the color of the indicator reflects the pH of the headspace of the beef packaging. Beef was stored at 20 °C for 24 h, after which the color of the freshness indicator changed. Results of color change from freshness indicator are similar to quality parameters including chemical, microbiological, and sensory evaluation that are commonly used to characterize the freshness of beef. The results show that this freshness indicator is useful for determining the shelf life of beef products by monitoring physicochemical changes and microbial safety.
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References
Al-Masri M, Al-Bachir M. Microbial load, acidity, lipid oxidation and volatile basic nitrogen of irradiated fish and meat-bone meals. Biores. Technol. 98: 1163–1166 (2007)
Biji KB, Ravishankar CN, Mohan CO, Srinivasa Gopal TK. Smart packaging systems for food applications: a review. J Food Sci. Technol. 52: 6125–6135 (2015)
Byrne L, Lau KT, Diamond D. Monitoring of headspace total volatile basic nitrogen from selected fish species using reflectance spectroscopic measurements of pH sensitive films. The Analyst 127: 1338–1341 (2002)
Byun J-S, Min JS, Kim IS, Kim J-W, Chung M-S, Lee M. Comparison of Indicators of Microbial Quality of Meat during Aerobic Cold Storage. J. Food Prot. 66: 1733–1737 (2003)
Castro P, Padron JCP, Cansino MJC, Velázquez ES, De Larriva RM. Total volatile base nitrogen and its use to assess freshness in European sea bass stored in ice. Food Control 17: 245–248 (2006)
Chan ST, Yao MW, Wong Y, Wong T, Mok C, Sin DW. Evaluation of chemical indicators for monitoring freshness of food and determination of volatile amines in fish by headspace solid-phase microextraction and gas chromatography-mass spectrometry. Euro. Food Res. Technol. 224: 67–74 (2006)
Chen H-Z, Zhang M, Bhandari B, Guo Z. Applicability of a colorimetric indicator label for monitoring freshness of fresh-cut green bell pepper. Posthar. Biol. Technol. 140: 85–92 (2018)
Chun H-N, Kim B, Shin H-S. Evaluation of a freshness indicator for quality of fish products during storage. Food Sci. Biotechnol. 23: 1719–1725 (2014)
Chun J-Y, Choi M-J, Lee SJ, Hong G-P. Applications of time-temperature integrator (TTI) as a quality indicator of grounded pork patty. Korean J. Food Sci. Animal Res. 33: 439–447 (2013)
Florek M, Litwinczuk A, Skalecki P, Ryszkowska-Siwko M. Changes of physicochemical properties of bullocks and heifers meat during 14 days of ageing under vacuum. Polish J. Food Nutr. Sci. 57: 281–287 (2007)
Ezati P, Tajik H, Moradi M. Fabrication and characterization of alizarin colorimetric indicator based on cellulose-chitosan to monitor the freshness of minced beef. Sens. Actuat. B: Chemical 285: 519–528 (2019)
Kim I, Lee S, Lee M, Lee J, Kim J, Kim Y. Physicochemical, Microbiological, and Sensory Characteristics of Chilled Chuck Rolls of Han-Woo and Imported Beef. Korean J. Animal Sci. 42: 109–116 (2000)
Kim K, Kim E, Lee SJ. New enzymatic time–temperature integrator (TTI) that uses laccase. J. Food Eng. 113: 118–123 (2012a)
Kim M-J, Shin H-S. Effect of treatment with ozonated water on shelf life of refrigerated meat. Korean J. Food Sci. Animal Res. 31: 617–623 (2011)
Kim Y-A, Jung S-W, Park H-R, Chung K-Y, Lee S-J. Application of a Prototype of Microbial Time Temperature Indicator (TTI) to the Prediction of Ground Beef Qualities during Storage. Korean J. Food Sci. of Animal Res. 32: 448–457 (2012b)
Kuswandi B, Damayanti F, Jayus J, Abdullah A, Heng LY. Simple and Low-Cost On-Package Sticker Sensor based on Litmus Paper for Real-Time Monitoring of Beef Freshness. J. Math. Fund. Sci. 47: 236–251 (2015)
Kuswandi B, Nurfawaidi A. On-package dual sensors label based on pH indicators for real-time monitoring of beef freshness. Food Control 82: 91–100 (2017)
Lee K, Baek S, Kim D, Seo J. A freshness indicator for monitoring chicken-breast spoilage using a Tyvek® sheet and RGB color analysis. Food Pack. Shelf Life 19: 40–46 (2019)
Lee S-Y, Lee J-Y, Shin H-S. Evaluation of chemical analysis method and determination of polycyclic aromatic hydrocarbons content from seafood and dairy products. Toxicol. Research 31: 265 (2015)
Min JS, Lee SO, Jang A, Jo C, Park CS, Lee M. Relationship between the Concentration of Biogenic Amines and Volatile Basic Nitrogen in Fresh Beef, Pork, and Chicken Meat. Asian-Australas J Anim Sci 20: 1278–1284 (2007)
Pacquit A, Frisby J, Diamond D, Lau K, Farrell A, Quilty B, Diamond D. Development of a smart packaging for the monitoring of fish spoilage. Food Chem. 102: 466–470 (2007)
Park HJ, Lee MJ, Lee HR. Effects of temperature-fluctuation in a refrigerator on antioxidative index and storage qualities of various foods. J. Nutr. Health 50: 133–141 (2017)
Rukchon C, Nopwinyuwong A, Trevanich S, Jinkarn T, Suppakul P. Development of a food spoilage indicator for monitoring freshness of skinless chicken breast. Talanta 130: 547–554 (2014)
Sabow AB, Sazili AQ, Aghwan ZA, Zulkifli I, Goh YM, Ab Kadir MZ, Nakyinsige K, Kaka U, Adeyemi KD. Changes of microbial spoilage, lipid-protein oxidation and physicochemical properties during post mortem refrigerated storage of goat meat. J. Anim. Sci. 87: 816–826 (2016)
Shin H-Y, Ku K-J, Park S-K, Song K-B. Use of freshness indicator for determination of freshness and quality change of tofu during storage. Appl. Biol. Chem. 49: 158–162 (2006)
Shukla V, Kandeepan G, Vishnuraj MR. Development of on package indicator sensor for real-time monitoring of meat quality. Vet. World 8: 393–397 (2015)
Singhal RS, Kulkarni P, Reg D. Handbook of indices of food quality and authenticity. Elsevier (1997)
Sun Q, Faustman C, Senecal A, Wilkinson A, Furr H. Aldehyde reactivity with 2-thiobarbituric acid and TBARS in freeze-dried beef during accelerated storage. Meat Sci. 57: 55–60 (2001)
Vaikousi H, Biliaderis CG, Koutsoumanis KP. Applicability of a microbial Time Temperature Indicator (TTI) for monitoring spoilage of modified atmosphere packed minced meat. Int. J. Food Microbiol. 133: 272–278 (2009)
Wohlsen T, Bates J, Vesey G, Robinson WA, Katouli M. Evaluation of the methods for enumerating coliform bacteria from water samples using precise reference standards. Lett. Appl. Microbiol. 42: 350–356 (2006)
Xiao-wei H, Zhi-hua L, Xiao-bo Z, Ji-yong S, Han-ping M, Jie-wen Z, Li-min H, Holmes M. Detection of meat-borne trimethylamine based on nanoporous colorimetric sensor arrays. Food Chem. 197: 930–936 (2016)
Yam KL, Takhistov PT, Miltz J. Intelligent packaging: concepts and applications. J. Food Sci. 70: R1–R10 (2005)
Acknowledgements
This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through the Agricultural Research Center Project, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA; 710013-03-2-SB120).
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Lee, EJ., Shin, HS. Development of a freshness indicator for monitoring the quality of beef during storage. Food Sci Biotechnol 28, 1899–1906 (2019). https://doi.org/10.1007/s10068-019-00633-5
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DOI: https://doi.org/10.1007/s10068-019-00633-5