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Proteomic response of Saccharomyces boulardii to simulated gastrointestinal conditions and encapsulation

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Abstract

Probiotics are live microorganisms conferring health benefits when administered in adequate amounts. However, the passage through the gastrointestinal tract represents a challenge due to pH variations, proteases, and bile salts. This study aimed to evaluate the proteomic response of Saccharomyces boulardii to simulated gastrointestinal digestion and the influence of encapsulation on yeast viability. Different pH values and time periods simulating the passage through different sections of the gastrointestinal tract were applied to unencapsulated and encapsulated yeasts. Encapsulation in 0.5% calcium alginate did not improve yeast survival or induce changes in protein patterns whereas protein extracts from control and digested yeasts showed remarkable differences when separated by SDS-PAGE. Protein bands were analyzed by tandem mass spectrometry. Protein identification revealed unique proteins that changed acutely in abundance after simulated digestion. Carbohydrate metabolism, protein processing, and oxide-reduction were the biological processes most affected by simulated gastrointestinal digestion in S. boulardii.

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Acknowledgements

Thanks to CONACYT-Mexico for awarding us Grant-251744-Infrastructure. Thanks to Plataforma Analítica Institucional-Centro de Investigación en Alimentación y Desarrollo, A.C. (Project PAI-10363). We also thank CONACYT-Mexico Project CB-169358. Martha Beatriz Morales-Amparano thanks CONACYT-Mexico for her MSc fellowship (713677).

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Authors and Affiliations

  1. Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera Gustavo Enrique Astiazarán Rosas No. 46, Apartado Postal 1735, 83304, Hermosillo, Sonora, Mexico

    Martha Beatriz Morales-Amparano, Gabriela Ramos-Clamont Montfort, Itzamná Baqueiro-Peña, María del Refugio Robles-Burgueño & Luz Vázquez-Moreno

  2. CONACYT-Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán Rosas No. 46, Apartado Postal 1735, 83304, Hermosillo, Sonora, Mexico

    José Ángel Huerta-Ocampo

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  1. Martha Beatriz Morales-Amparano
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  2. Gabriela Ramos-Clamont Montfort
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  3. Itzamná Baqueiro-Peña
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  6. José Ángel Huerta-Ocampo
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Correspondence to José Ángel Huerta-Ocampo.

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Morales-Amparano, M.B., Ramos-Clamont Montfort, G., Baqueiro-Peña, I. et al. Proteomic response of Saccharomyces boulardii to simulated gastrointestinal conditions and encapsulation. Food Sci Biotechnol 28, 831–840 (2019). https://doi.org/10.1007/s10068-018-0508-9

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  • DOI: https://doi.org/10.1007/s10068-018-0508-9

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