Infusion efficiency of sodium fluorescein into various starches


The objective of this study was to develop new drug delivery systems (DDS) and nutrient delivery systems (NDS), using starch as a carrier material for infusion technology. Corn, waxy rice, non-waxy rice, and potato starches were used as carrier materials. Sodium fluorescein was used as an infusion material for easy detection. Each starch suspension with sodium fluorescein was reacted in a water bath at 40, 50, and 60 °C for 30 min. After each reaction, the concentration of sodium fluorescein in the supernatant was measured using a fluorescence detector. Precipitated starch was observed using fluorescence microscopy. About 70% of sodium fluorescein infused in waxy rice and corn starches at 60 °C. Additionally, the granules of these two starches were luminous by green light when exposed to a fluorescence detector, suggesting that corn and waxy rice starches can be used as carrier materials in infusion technology for DDS and NDS.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2


  1. Baldwin PM, Adler J, Davies MC, Melia CD. Holes in starch granules: confocal, SEM and light microscopy studies of starch granule structure. Starch/Stärke 46: 341–346 (1994)

    CAS  Article  Google Scholar 

  2. Choi HW, Chung KM, Kim CH, Moon TH, Kim DS, Park CS, Baik MY. Physicochemical properties of cross-linked rice starches. J. Korean Soc. Appl. Biol. Chem. 49: 49–54 (2006)

    CAS  Google Scholar 

  3. Fannon JE, Gray JA, Gunawan N, Huber KC, BeMiller JN. The channels of starch granules. Food Sci. Biotechnol. 12: 700–704 (2003)

    CAS  Google Scholar 

  4. Fehr M, Frommer WB, Lalonde S. Visualization of maltose uptake in living yeast cells by fluorescent nanosensors. Proc. Natl. Acad. Sci. USA 99: 9846–9851 (2002)

    CAS  Article  Google Scholar 

  5. Han S, Choi SH, Kim BY, Baik MY. Infusion of catechin into native corn starch granule for drug and nutrient delivery systems. Food Sci. Biotechnol. 24: 2035–2040 (2015)

    CAS  Article  Google Scholar 

  6. He H, Mortellaro MA, Leiner MJ, Fraatz RJ, Tusa JK. A fluorescent sensor with high selectivity and sensitivity for potassium in water. J. Am. Chem. Soc. 125: 1468–1469 (2003)

    CAS  Article  Google Scholar 

  7. Herrero-Vanrell R, Refojo MF. Biodegradable microspheres for vitreoretinal drug delivery. Adv. Drug Deliv. Rev. 52: 5–16 (2001)

    CAS  Article  Google Scholar 

  8. Huber KC, BeMiller JN. Visualization of channels and cavities of corn and sorghum starch granules. Cereal Chem. 74: 537–541 (1997)

    CAS  Article  Google Scholar 

  9. Huber KC, BeMiller JN. Channels of maize and sorghum starch granules. Carbohydr. Polym. 41: 269–276 (2000)

    CAS  Article  Google Scholar 

  10. Kim HS, Huber KC. Channels within soft wheat starch A- and B-type granules. J. Cereal Sci. 48: 159–172 (2008)

    CAS  Article  Google Scholar 

  11. Panyam J, Labhasetwar V. Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv. Drug Deliv. Rev. 55: 329–347 (2003)

    CAS  Article  Google Scholar 

  12. Stojanovic, MN, Prada, P, Landry, DW. Flurescent sensors based on aptamer self-assembly. J. Am. Chem. Soc. 122: 11547–11548 (2000)

    CAS  Article  Google Scholar 

Download references


This research was supported by the Main Research Program (E0164800-03) of the Korea Food Research Institute (KFRI) funded by the Ministry of Science, ICT & Future Planning.

Author information



Corresponding author

Correspondence to Moo-Yeol Baik.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Choi, SH., Kim, Hy., Oh, SM. et al. Infusion efficiency of sodium fluorescein into various starches. Food Sci Biotechnol 28, 99–102 (2019).

Download citation


  • Drug delivery system
  • Nutrient delivery system
  • Infusion
  • Starch
  • Sodium fluorescein